Abstracts tagged "Animal models"

Abstract Number: 2228 • 2013 ACR/ARHP Annual Meeting
FMS-Like Tyrosine Kinase 3 Ligand -Dependent CD103+DC Are Crucial For The Initiation Of Collagen-Induced Arthritis
Maria Ines Ramos¹, Samuel Garcia², Saïda Aarrass¹, Boy Helder³, Kris A. Reedquist⁴, Paul-Peter Tak⁵ and Maria C. Lebre¹, ¹Clinical Immunology and Rheumatology & Experimental Immunology, Academic Medical Center, University of Amsterdam, Amsterdam, Netherlands, ²Department of Experimental Immunology, Division of Clinical Immunology and Rheumatology, Academic Medical Center/University of Amsterdam, Amsterdam, Netherlands, ³Clinical Immunology and Rheumatology & Experiemental Immunology, Academic Medical Center, University of Amsterdam, Amsterdam, Netherlands, ⁴Department of Experimental Immunology, Division of Clinical Immunology and Rheumatology, Academic Medical Center, University of Amsterdam, Amsterdam, Netherlands, ⁵Division of Clinical Immunology and Rheumatology, Academic Medical Center, University of Amsterdam, Amsterdam, Netherlands
Background/Purpose: Dendritic cells (DCs) are a heterogeneous cell population that plays an important role for the initiation of protective and pathogenic immunity. Flt3L is a…
Abstract Number: 1251 • 2013 ACR/ARHP Annual Meeting
Collagen Antibodies Induce Pain-Like Behavior In Mice Without Signs Of Inflammation
Gustaf Wigerblad¹, Katalin Sandor¹, Kutty Selva Nandakumar², Rikard Holmdahl³ and Camilla Svensson¹, ¹Department of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden, ²Department of Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden, ³Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm, Sweden
Background/Purpose: Collagen Antibody Induced Arthritis (CAIA) is a mouse model of rheumatoid arthritis (RA). It is induced by injection of a cocktail of monoclonal antibodies…
Abstract Number: 148 • 2013 ACR/ARHP Annual Meeting
Microarray Analysis Of Synovial Specimen of Early Human (CHECK) and Experimental Osteoarthritis to Identify Pathways and Processes Associated with Pathology
Arjen B. Blom¹, Peter L. van Lent², Martijn H. van den Bosch¹, Hans Cats³, Frank H.J. van den Hoogen⁴, Peter M. van der Kraan⁵ and Wim B. van den Berg⁶, ¹Rheumatology Research & Advanced Therapeutics, Radboud University Nijmegen Medical Centre, Nijmegen, Netherlands, ²Rheumatology Research & Advanced Therpeutics, Radboud University Nijmegen Medical Centre, Nijmegen, Netherlands, ³Rheumatology, Rheumatology Centre Sint Maartenskliniek, Nijmegen, Netherlands, ⁴Rheumatology, Rheumatology Centre Sint Maartenskliniek and Radboud university medical center, Ubbergen (Nijmegen), Netherlands, ⁵Rheumatology Research and Advanced Therapeutics, Radboud University Nijmegen Medical Centre, Nijmegen, Netherlands, ⁶Rheumatology Research and Advanced Therapeutics, Department of Rheumatology, Radboud University Nijmegen Medical Centre, Nijmegen, Netherlands
Background/Purpose: The majority of osteoarthritis (OA) patients show synovial inflammation, even relatively early during the disease. We used microarray analysis of synovial tissue of early…
Abstract Number: 32 • 2012 ACR/ARHP Annual Meeting
Development of a Quantitative Model of Endotoxin-Induced Calvarial Bone Erosion Utilizing Micro-Computed Tomography and Pax-It Imaging Analysis Software
Diane Thome, Donald Souza, Aruna Behera, Jie Zheng, Jennifer Swantek and Gerald H. Nabozny, Immunology and Inflammation, Boehringer Ingelheim Pharmaceuticals Inc., Ridgefield, CT
Background/Purpose: To facilitate an understanding of the biological factors involved in inflammation- mediated bone loss, in vitro systems and murine models of bone loss, such…
Abstract Number: 731 • 2012 ACR/ARHP Annual Meeting
A Systems Biology Approach to Elucidating Pathways Active During the Development of Osteoarthritis
Richard F. Loeser¹, Amy L. Olex², Brian Westwood², Margaret A. McNulty³, Cathy S. Carlson³, Michael Callahan⁴, Cristin Ferguson⁴ and Jacquelyn S. Fetrow², ¹Section Of Molecular Medicine, Wake Forest School of Medicine, Winston-Salem, NC, ²Computer Science, Wake Forest University, Winston-Salem, NC, ³Veterinary and Diagnostic Medicine, University of Minnesota, St. Paul, MN, ⁴Orthopedic Surgery, Wake Forest School of Medicine, Winston-Salem, NC
Background/Purpose: OA affects the entire joint but most studies have focused on the disease process in a single tissue. In this study, we identified genes…
Abstract Number: 1440 • 2012 ACR/ARHP Annual Meeting
Lupus-Prone Mice Demonstrate Enhanced Neutrophil Extracellular Trap Formation: Implications for Autoantibody Formation and Organ Damage
Jason S. Knight¹, Alexander A. O'Dell¹, Wenpu Zhao², Ritika Khandpur², Srilakshmi Yalavarthi² and Mariana J. Kaplan³, ¹Department of Internal Medicine, Division of Rheumatology, University of Michigan, Ann Arbor, MI, ²University of Michigan Rheumatology, Ann Arbor, MI, ³Systemic Autoimmunity Branch, National Institutes of Health/NIAMS, Bethesda, MD
Background/Purpose: Recent evidence suggests that enhanced neutrophil extracellular trap (NET) formation—the programmed release of chromatin fibers decorated with granular antimicrobial peptides—is as an important activator…
Abstract Number: 2086 • 2012 ACR/ARHP Annual Meeting
PI3-Kinase Controls Inflammatory Bone Destruction by Regulating the Osteoclastogenic Potential of Myeloid Cells
Stephan Blueml¹, Gernot Schabbauer², Antonia Puchner³, Emine Sahin², Victoria Saferding⁴, Birgit Niederreiter⁵, Josef S. Smolen⁶ and Kurt Redlich⁵, ¹Division of Rheumatology, Medical University of Vienna, Vienna, Austria, ²Vascular Biology and Thrombosis research, Medical University Vienna, Vienna, Austria, ³Dpt of Rheumatology, Medical University Vienna, Vienna, Austria, ⁴Rheumatology, Medical University of Vienna, Vienna, Austria, ⁵Department of Internal Medicine III, Division of Rheumatology, Medical University of Vienna, Vienna, Austria, ⁶Division of Rheumatology, Department of Internal Medicine III,, Medical University of Vienna and Hietzing Hospital, Vienna, Austria
Background/Purpose: Local bone destruction in rheumatoid arthritis, psoriasisarthritis or ankylosing spondylitis is a serious health burden and the major cause of disability and severely reduced…
Abstract Number: 35 • 2012 ACR/ARHP Annual Meeting
Changes in Tibial Bone and Cartilage Structure in a Mouse Surgical Model of Osteoarthritis
Brett A. Tonkin¹, Evange Romas², Natalie A. Sims¹ and Nicole C. Walsh¹, ¹St Vincent's Institute of Medical Research, Melbourne, Australia, ²Dept. of Rheumatology, St Vincent's Hospital, Melbourne, Australia
Background/Purpose: The destabilisation of the medial meniscus (DMM) mouse osteoarthritis (OA) model is commonly used to study OA joint degeneration. In DMM-OA, the knee is…
Abstract Number: 738 • 2012 ACR/ARHP Annual Meeting
A Novel Orally Active Phosphatidylinositol 3-Phosphate 5-Kinase (PIKfyve) Inhibitor Ameliorates Mouse Psoriasis-Like Model by Inhibition of Interleukin-12 and Interleukin-23 Production From Macrophages
Ayatoshi Andou, Eviryanti Agung, Yukie Seki, Yoichiro Shima, Sen Takeshita, Takashi Yamamoto and Hiroyuki Eda, Exploratory Research Laboratories, Ajinomoto Pharmaceuticals Co., Ltd., Kanagawa, Japan
Background/Purpose: Phosphatidylinositol (3,5)-bisphosphate (PI(3,5)P2) is the most recently-identified phospholipid component of cellular membrane. Phosphatidylinositol 3-phosphate 5-kinase (PIKfyve) is a critical enzyme for the synthesis of…
Abstract Number: 1513 • 2012 ACR/ARHP Annual Meeting
TSLP Receptor Deficiency Reduces IL-13 Expression and Prevents Fibrosis in Experimental Scleroderma
Alicia Usategui¹, Vanessa Miranda¹, Gabriel Criado², Manuel J. Del Rey¹, Elena Izquierdo¹, Warren J. Leonard³ and Jose L. Pablos¹, ¹Servicio de Reumatología, Instituto de Investigación Hospital 12 de Octubre (I+12), Madrid, Spain, ²Grupo de Enfermedades Inflamatorias y Autoinmunes, Instituto de Investigación Hospital 12 de Octubre (I+12), Madrid, Spain, ³Laboratory of Molecular Immunology and Immunology Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD
Background/Purpose: Systemic sclerosis (SSc) is an autoimmune disease characterized by progressive fibrosis of the skin and internal organs. Although SSc shares pathogenetic features with other…
Abstract Number: 2085 • 2012 ACR/ARHP Annual Meeting
T Cell-Mediated Murine Antigen-Induced Arthritis Is Resistant to Transgenic Disruption of Glucocorticoid Signaling in Osteoblasts and Osteocytes in Vivo
Cornelia M. Spies¹, Edgar Wiebe¹, Jinwen W. Tu², Aiqing Li², Timo Gaber¹, Dörte Huscher¹, Markus J. Seibel², Hong Zhou² and Frank Buttgereit³, ¹Department of Rheumatology and Clinical Immunology, Charité University Medicine, Berlin, Germany, ²Bone Research Program, ANZAC Research Institute, The University of Sydney, Concord, Australia, ³Charité - Universitätsmedizin Berlin, Berlin, Germany
Background/Purpose: The role of endogenous glucocorticoids (GC) in, and their contribution to the susceptibility and severity of rheumatoid arthritis remains inconclusive. We previously demonstrated that…
Abstract Number: 37 • 2012 ACR/ARHP Annual Meeting
Development of a Mouse Model of Natural Osteoarthritis of Knee by Induction of obesity and Bipedal Walking
Hyun Ah Kim¹, Su Jin Park², Eun-Jeong Cheon³, Hyun A. Jung⁴ and Kyeong Min Son⁵, ¹Division of Rheumatology, Department of Internal Medicine, Hallym university, Kyunggi, South Korea, ²Department of Internal Medicine, Hallym University Sacred Heart Hospital, Kyunggi, South Korea, ³Department of internal medicine, Hallym University Sacred Heart Hospital, Kyunggi, South Korea, ⁴Division of Rheumatology, Department of Internal Medicine, Hallym university sacred heart hospital, Kyunggi, South Korea, ⁵Division of Rheumatology, Department of Internal Medicine, Hallym university Chunchun sacred heart hospital, Chunchun, South Korea
Background/Purpose: Animal models of osteoarthritis(OA) are used extensively in research of its pathogenesis and in search of disease modifying anti-OA drugs. However, whether current animal models…
Abstract Number: 734 • 2012 ACR/ARHP Annual Meeting
Chemokine (C-C Motif) Receptor 2 Signaling Mediates Persistent Pain in Experimental Osteoarthritis
Rachel E. Miller¹, Phuong Tran², Rosalina Das², Nayereh Ghoreishi-Haack², Richard J. Miller³ and Anne-Marie Malfait⁴, ¹Rheumatology/Biochemistry, Rush University Medical Center, Chicago, IL, ²Rheumatology, Rush University Medical Center, Chicago, IL, ³Molecular Biochemistry and Pharmacology, Northwestern University, Chicago, IL, ⁴Internal Medicine/Biochemistry, Rush University Medical Center, Chicago, IL
Background/Purpose: To investigate the role of MCP-1/CCR2 in the development of pain in osteoarthritis (OA) using a mouse model, destabilization of the medial meniscus (DMM).…
Abstract Number: 1507 • 2012 ACR/ARHP Annual Meeting
Propylthiouracil Reduces Fibrosis in Chronic Oxidant Stress Mouse Model of Scleroderma
Gianluca Bagnato¹, Alessandra Bitto², Natasha Irrera², Gabriele Pizzino², Neal Roberts³, Maurizio Cinquegrani⁴, Donatella Sangari¹, Francesco Squadrito², Gianfilippo Bagnato¹ and Antonino Saitta⁴, ¹Rheumatology, University of Messina, Messina, Italy, ²Pharmacology, University of Messina, Messina, Italy, ³Medical College of Virginia, Richmond, VA, ⁴Internal Medicine, University of Messina, Messina, Italy
Background/Purpose: Systemic sclerosis (SSc) is characterized by fibrosis of skin and visceral organs; vascular damage and resultant ischemia; and immunological dysregulation manifested by autoantibodies. Hypothyroidism,…
Abstract Number: 2084 • 2012 ACR/ARHP Annual Meeting
ASP015K: A Novel JAK Inhibitor Demonstrated Potent Efficacy in Adjuvant-Induced Arthritis Model in Rats
Shunji Yamazaki¹, Masamichi Inami¹, Misato Ito¹, Yasutomo Fujii¹, Kaori Hanaoka¹, Kaoru Yamagami¹, Kenji Okuma¹, Yoshiaki Morita¹, Shohei Shirakami², Takayuki Inoue², Susumu Miyata³ and Yasuyuki Higashi¹, ¹Pharmacology Research Labs, Astellas Pharma Inc., Tsukuba, Japan, ²Chemistry Research Labs, Astellas Pharma Inc., Tsukuba, Japan, ³Research Planning & Administration, Astellas Pharma Inc., Tsukuba, Japan
Background/Purpose: The Janus kinase (JAK) family of enzymes plays a key role in cytokine signaling, which is involved in the pathogenic events of immune-mediated disorders…