ACR Meeting Abstracts

ACR Meeting Abstracts

Abstract Number: 0784

Upregulation of Tyro3TK on CD14+CD16- Monocytes Promotes Osteoclast Formation in Rheumatoid Arthritis

Jimeng Xue1, Liling Xu1, Fanlei Hu1 and Yin Su2, 1Department of Rheumatology and Immunology, Peking University People’s Hospital, Beijing Key Laboratory for Rheumatism Mechanism and Immune Diagnosis (BZ0135), Beijing, China, Beijing, China (People's Republic), 2Department of Rheumatology and Immunology, Peking University People’s Hospital, Beijing, China (People's Republic)

Meeting: ACR Convergence 2020

Keywords: , ,

Session Information

Date: Saturday, November 7, 2020

Session Title: RA – Etiology & Pathogenesis Poster

Session Type: Poster Session B

Session Time: 9:00AM-11:00AM

Background/Purpose: The study aimed to investigate the expression and clinical significance of Tyro3TK on CD14+CD16+ and CD14+CD16– monocyte subsets and explore the effect of Tyro3TK on osteoclast formation in rheumatoid arthritis (RA).

Methods: Osteoclasts were induced by CD14+CD16+ and CD14+CD16– monocyte subsets isolated from healthy control (HC) and RA in vitro, and evaluated by tartrate-resistant acid phosphatase (TRAP) staining. Then, the expression of Tyro3TK on CD14+CD16+ and CD14+CD16– monocyte subsets were evaluated in peripheral blood of RA by flow cytometry, and the correlation between the expression of Tyro3TK on CD14+CD16+ and CD14+CD16– monocyte subsets with RA patient clinical data were analyzed. At last, the role of Tyro3TK on CD14+CD16– monocyte in RA patient osteoclastogenesis was further performed by osteoclast differentiation assay.

Results: The results revealed that CD14+CD16– monocytes were the main source of osteoclasts. The expression of Tyro3TK on CD14+CD16– monocytes was significantly upregulated in RA patients as compared with HC and osteoarthritis (OA) patients, which was positively correlated with the disease manifestations. Moreover, anti-Tyro3TK antibody could dose-dependently inhibited Gas6-mediated osteoclast differentiation in CD14+CD16– monocytes.

Conclusion: These findings indicate that elevated Tyro3TK on CD14+CD16– monocytes serves as a critical signal for osteoclast differentiation in RA, but its mechanism needs to be further studied.

The expression of Tyro3TK on CD14+CD16- monocytes is increased in RA. (A) The expression of Tyro3TK on CD14+CD16+ and CD14+CD16- monocytes in HC (n = 40), OA (n = 28), and RA patients (n = 40, **P = 0.008) were analyzed, and presented as mean fluorescence intensity (MFI). (B) The expression of Tyro3TK on CD14+CD16+ monocytes and CD14+CD16- monocytes were compared between HC, OA, and RA patients (**P = 0.008, ***P < 0.001).

Disclosure: J. Xue, None; L. Xu, None; F. Hu, None; Y. Su, None.

To cite this abstract in AMA style:

Xue J, Xu L, Hu F, Su Y. Upregulation of Tyro3TK on CD14+CD16- Monocytes Promotes Osteoclast Formation in Rheumatoid Arthritis [abstract]. Arthritis Rheumatol. 2020; 72 (suppl 10). https://acrabstracts.org/abstract/upregulation-of-tyro3tk-on-cd14cd16-monocytes-promotes-osteoclast-formation-in-rheumatoid-arthritis/. Accessed January 17, 2021.

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ACR Meeting Abstracts - https://acrabstracts.org/abstract/upregulation-of-tyro3tk-on-cd14cd16-monocytes-promotes-osteoclast-formation-in-rheumatoid-arthritis/