Session Type: Poster Session B
Session Time: 9:00AM-11:00AM
Background/Purpose: The study aimed to investigate the expression and clinical significance of Tyro3TK on CD14+CD16+ and CD14+CD16– monocyte subsets and explore the effect of Tyro3TK on osteoclast formation in rheumatoid arthritis (RA).
Methods: Osteoclasts were induced by CD14+CD16+ and CD14+CD16– monocyte subsets isolated from healthy control (HC) and RA in vitro, and evaluated by tartrate-resistant acid phosphatase (TRAP) staining. Then, the expression of Tyro3TK on CD14+CD16+ and CD14+CD16– monocyte subsets were evaluated in peripheral blood of RA by flow cytometry, and the correlation between the expression of Tyro3TK on CD14+CD16+ and CD14+CD16– monocyte subsets with RA patient clinical data were analyzed. At last, the role of Tyro3TK on CD14+CD16– monocyte in RA patient osteoclastogenesis was further performed by osteoclast differentiation assay.
Results: The results revealed that CD14+CD16– monocytes were the main source of osteoclasts. The expression of Tyro3TK on CD14+CD16– monocytes was significantly upregulated in RA patients as compared with HC and osteoarthritis (OA) patients, which was positively correlated with the disease manifestations. Moreover, anti-Tyro3TK antibody could dose-dependently inhibited Gas6-mediated osteoclast differentiation in CD14+CD16– monocytes.
Conclusion: These findings indicate that elevated Tyro3TK on CD14+CD16– monocytes serves as a critical signal for osteoclast differentiation in RA, but its mechanism needs to be further studied.
The expression of Tyro3TK on CD14+CD16- monocytes is increased in RA. (A) The expression of Tyro3TK on CD14+CD16+ and CD14+CD16- monocytes in HC (n = 40), OA (n = 28), and RA patients (n = 40, **P = 0.008) were analyzed, and presented as mean fluorescence intensity (MFI). (B) The expression of Tyro3TK on CD14+CD16+ monocytes and CD14+CD16- monocytes were compared between HC, OA, and RA patients (**P = 0.008, ***P < 0.001).
To cite this abstract in AMA style:Xue J, Xu L, Hu F, Su Y. Upregulation of Tyro3TK on CD14+CD16- Monocytes Promotes Osteoclast Formation in Rheumatoid Arthritis [abstract]. Arthritis Rheumatol. 2020; 72 (suppl 10). https://acrabstracts.org/abstract/upregulation-of-tyro3tk-on-cd14cd16-monocytes-promotes-osteoclast-formation-in-rheumatoid-arthritis/. Accessed January 16, 2022.
« Back to ACR Convergence 2020
ACR Meeting Abstracts - https://acrabstracts.org/abstract/upregulation-of-tyro3tk-on-cd14cd16-monocytes-promotes-osteoclast-formation-in-rheumatoid-arthritis/