ACR Meeting Abstracts

ACR Meeting Abstracts

  • Meetings
    • ACR Convergence 2024
    • ACR Convergence 2023
    • 2023 ACR/ARP PRSYM
    • ACR Convergence 2022
    • ACR Convergence 2021
    • ACR Convergence 2020
    • 2020 ACR/ARP PRSYM
    • 2019 ACR/ARP Annual Meeting
    • 2018-2009 Meetings
    • Download Abstracts
  • Keyword Index
  • Advanced Search
  • Your Favorites
    • Favorites
    • Login
    • View and print all favorites
    • Clear all your favorites
  • ACR Meetings

Abstract Number: 0070

Shared and Unique Fecal and Plasma Metabolic Signatures and Increased Inflammatory Markers in Ankylosing Spondylitis, Psoriatic Arthritis and Acute Anterior Uveitis

Sneha Couvillion1, Javier Flores1, Sean Davin2, Kimberly Ogle2, Emma Fale-Olsen2, John Davis2, Gina Many1, Carley Shaut2, Tammy Martin2, Eric Suhler2, Atul Deodhar3, James Rosenbaum4, Ernesto Nakayasu1 and Tejpal Gill2, 1PNNL, Richland, WA, 2OHSU, Portland, OR, 3Division of Arthritis and Rheumatic Diseases, Oregon Health & Science University, Portland, OR, 4Corvus Pharmaceuticals and Legacy Devers Eye Institute, Portland, OR

Meeting: ACR Convergence 2024

Keywords: Ankylosing spondylitis (AS), Biomarkers, Inflammation, metabolomics, Psoriatic arthritis

  • Tweet
  • Click to email a link to a friend (Opens in new window) Email
  • Click to print (Opens in new window) Print
Session Information

Date: Saturday, November 16, 2024

Title: SpA Including PsA – Basic Science Poster

Session Type: Poster Session A

Session Time: 10:30AM-12:30PM

Background/Purpose: Spondyloarthritis (SpA) is a group of immune-mediated inflammatory disorders such as ankylosing spondylitis (AS) and psoriatic arthritis (PsA), along with acute anterior uveitis (AAU) which is a common comorbidity of SpA. We aim to determine the fecal and plasma metabolic profiles to elucidate metabolic function underlying disease pathophysiology.

Methods: We performed untargeted metabolic profiling using liquid chromatography-mass spectrometry (LC-MS) on 103 fecal (19 AS, 18 AAU, 14 AS+AAU, 26 PsA, and 26 healthy controls [HC]) and 114 plasma (21 AS, 21 AAU, 20 AS+AAU, 26 PsA and 38 HC) samples. Metabolic data were analyzed using discriminatory statistical methods to dissect disease-associated metabolic signals and various confounders i.e. BMI and HLA-B27 status., and reported after multiple tests corrections. Fecal and plasma marker of inflammation (calprotectin) and plasma levels of bacterial endotoxin (LPS) were also measured.

Results: Results: Metabolomic profiling of the fecal and plasma samples revealed shared and unique metabolic signatures in AS, PsA and AAU patients in comparison with HC (Fig. 1). The fecal samples from AS, AS+AAU, and PsA showed a significant increase in multiple inflammatory metabolites such as stearoyl-L-carnitine, palmitoylcarnitine, taurolithocholic acid, and biliverdin in comparison to HC, concomitant with a significant decrease in the levels of various anti-inflammatory metabolites including enterolactone and evoxine in comparison to HCs (Fig 2A).  Similarly, the plasma of all patient groups exhibited elevated levels of inflammatory metabolites such as phenylacetylglutamine and 3 indoxy sulphate, methioninesulphoxide, and adenine in comparison to HC (Fig 2B). Comparison between disease groups showed differences in intensities of metabolic perturbations in the carnitine, bile, tryptophan, and other anti-inflammatory markers. In addition, HC carrying HLA-B27 allele exhibit significant metabolic changes in fecal and plasma profiles in comparison with HLA-B27 negative HCs (Fig 1). Furthermore, all patient groups had significantly increased levels of plasma calprotectin, and plasma LPS levels in comparison with HC, while fecal calprotectin in AS, AAU, and AS+AAU were significantly increased in comparison to HC, but in PsA patients, the levels were similar to HCs (Fig. 3). Multi-omics network analysis to determine the association between altered fecal and plasma metabolic profile in all patient groups and its association with gut microbes, inflammatory markers and disease severity are underway.

Conclusion: We discovered both shared and unique fecal and plasma metabolic features associated with AS, PsA, AAU and AS+AAU in comparison with HCs, which has the potential to reveal why certain people develop one disease instead of the other despite their common association with HLA-B27. Future investigations validating the effects of these metabolites may lead to potential mediators of disease pathogenesis and identify novel metabolites as disease biomarkers and therapeutic strategies in SpA and other inflammatory diseases.

Supporting image 1

Figure 1: Altered metabolic profile in SpA and AAU. Volcano plots showing the metabolites with significantly increased (green) and decreased (red) intensities in (A) fecal and (B) plasma samples of patients with ankylosing spondylitis (AS), acute anterior uveitis (AAU), AS+AAU and psoriatic arthritis (PsA) in comparison with the healthy controls (HC), (C) metabolites from fecal and plasma samples in HLA-B27 positive HC in comparison to HLA-B27 negative HC. The data is presented after discriminatory statistics with disease, and HLA-B27, age, and BMI as confounding factors, after accounting for multiple tests corrections.

Supporting image 2

Figure 2: Increased metabolic intensity of inflammatory metabolites in fecal and plasma samples. Metabolic intensities of selected (A) fecal and (B) plasma metabolites in patients with ankylosing spondylitis (AS), acute anterior uveitis (AAU), AS+AAU in comparison with healthy controls (HC) are shown here. Each dot represents data from one subject. * P-val<0.05, ** P-val<0.01

Supporting image 3

Figure 3: Increased calprotectin levels in fecal and plasma samples from SpA and AAU patients. Fecal (left) calprotectin levels are significantly increased in patients with ankylosing spondylitis (AS), acute anterior uveitis (AAU), AS+AAU in comparison with healthy controls (HC); and plasma (right) levels are significantly increased in patients with AS, AAU, AS+AAU and psoriatic arthritis (PsA) in comparison with HC. The dots represent data from one HLa-B27 positive (red) subject or HLA-B27 negative (black) subject. * P-val<0.05, ** P-val<0.01, *** P-val<0.001, and **** P-val<0.0001


Disclosures: S. Couvillion: None; J. Flores: None; S. Davin: None; K. Ogle: None; E. Fale-Olsen: None; J. Davis: None; G. Many: None; C. Shaut: None; T. Martin: None; E. Suhler: None; A. Deodhar: AbbVie, 2, 5, 6, BMS, 2, 5, 6, Eli Lilly, 2, 5, 6, Janssen, 2, 5, 6, Novartis, 2, 5, 6, Pfizer, 2, 5, 6, UCB Pharma, 2, 5, 6; J. Rosenbaum: Corvus Pharmaceuticals, 3, 3, 11, 11; E. Nakayasu: None; T. Gill: None.

To cite this abstract in AMA style:

Couvillion S, Flores J, Davin S, Ogle K, Fale-Olsen E, Davis J, Many G, Shaut C, Martin T, Suhler E, Deodhar A, Rosenbaum J, Nakayasu E, Gill T. Shared and Unique Fecal and Plasma Metabolic Signatures and Increased Inflammatory Markers in Ankylosing Spondylitis, Psoriatic Arthritis and Acute Anterior Uveitis [abstract]. Arthritis Rheumatol. 2024; 76 (suppl 9). https://acrabstracts.org/abstract/shared-and-unique-fecal-and-plasma-metabolic-signatures-and-increased-inflammatory-markers-in-ankylosing-spondylitis-psoriatic-arthritis-and-acute-anterior-uveitis/. Accessed .
  • Tweet
  • Click to email a link to a friend (Opens in new window) Email
  • Click to print (Opens in new window) Print

« Back to ACR Convergence 2024

ACR Meeting Abstracts - https://acrabstracts.org/abstract/shared-and-unique-fecal-and-plasma-metabolic-signatures-and-increased-inflammatory-markers-in-ankylosing-spondylitis-psoriatic-arthritis-and-acute-anterior-uveitis/

Advanced Search

Your Favorites

You can save and print a list of your favorite abstracts during your browser session by clicking the “Favorite” button at the bottom of any abstract. View your favorites »

All abstracts accepted to ACR Convergence are under media embargo once the ACR has notified presenters of their abstract’s acceptance. They may be presented at other meetings or published as manuscripts after this time but should not be discussed in non-scholarly venues or outlets. The following embargo policies are strictly enforced by the ACR.

Accepted abstracts are made available to the public online in advance of the meeting and are published in a special online supplement of our scientific journal, Arthritis & Rheumatology. Information contained in those abstracts may not be released until the abstracts appear online. In an exception to the media embargo, academic institutions, private organizations, and companies with products whose value may be influenced by information contained in an abstract may issue a press release to coincide with the availability of an ACR abstract on the ACR website. However, the ACR continues to require that information that goes beyond that contained in the abstract (e.g., discussion of the abstract done as part of editorial news coverage) is under media embargo until 10:00 AM ET on November 14, 2024. Journalists with access to embargoed information cannot release articles or editorial news coverage before this time. Editorial news coverage is considered original articles/videos developed by employed journalists to report facts, commentary, and subject matter expert quotes in a narrative form using a variety of sources (e.g., research, announcements, press releases, events, etc.).

Violation of this policy may result in the abstract being withdrawn from the meeting and other measures deemed appropriate. Authors are responsible for notifying colleagues, institutions, communications firms, and all other stakeholders related to the development or promotion of the abstract about this policy. If you have questions about the ACR abstract embargo policy, please contact ACR abstracts staff at [email protected].

Wiley

  • Online Journal
  • Privacy Policy
  • Permissions Policies
  • Cookie Preferences

© Copyright 2025 American College of Rheumatology