ACR Meeting Abstracts

ACR Meeting Abstracts

Abstract Number: 2008

Vascular Adhesion Protein-1 (VAP-1) As Predictor of Radiographic Severity in Symptomatic Knee Osteoarthritis

Eirini Bournazou1, Jonathan Samuels2, Hua Zhou3, Svetlana Krasnokutsky Samuels4, Jyoti Patel5, Jenny Bencardino6, Leon Rybak7, Steven Abamson8, Uwe Junker9, Karen Brown1 and Mukundan Attur5, 1Roche Innovation Center, New York, NY, 2Department of Medicine, Divison of Rheumatology, NYU Langone Health, New York, NY, 3Bioinformatics, New York University, New York, NY, 4NYU School of Medicine, New York, NY, 5Rheumatology Research, NYU Langone Orthopedic Hospital, New York, NY, 6Radiology, NYU Langone Medical Center, New York, NY, 7Department of Radiology, NYU Langone Medical Center, New York, NY, 8Rheumatology, NYU Langone Health, New York, NY, 9Roche Innovation Center, Basel, Switzerland

Meeting: 2018 ACR/ARHP Annual Meeting

Keywords: , ,

Session Information

Date: Tuesday, October 23, 2018

Title: Osteoarthritis and Joint Biology – Basic Science Poster II

Session Type: ACR Poster Session C

Session Time: 9:00AM-11:00AM

Background/Purpose: To investigate the expression of vascular adhesion protein -1 (VAP-1) in joint tissues and serum in knee osteoarthritis (OA) patients and examine whether VAP-1 levels predict increased risk of disease severity or progression of knee OA.

Methods: Baseline serum and synovial fluid VAP-1/semicarbazide-sensitive amine oxidase (SSAO) levels were assessed in cohorts of patients with tibiofemoral medial knee OA and healthy subjects. Standardized fixed-flexion posteroanterior knee radiographs were scored for Kellgren Lawrence (KL) grade (0–4) and medial joint space width (JSW) at the mid-portion of the joint space. Radiographic severity was defined by KL2/3 vs. KL4. Biochemical markers assessed comprised VAP-1/SSAO, IL-1Ra, IL-6, sRAGE, CCL2, CCL4, CD163, hsCRP and MMPs-1,-3,-9. Associations between biomarkers and radiographic severity (logistic regression controlling for covariates) and pain (Spearman correlation) were evaluated.

Results: VAP-1 was locally overexpressed at least 2 fold in the OA synovium based on immunohistochemical, microarray and qRT-PCR analyses compared to controls. Synovial fluid SSAO levels was also significantly higher in OA (107.94+41.42) compared to normals (38.12 + 22.98 ng/ml; p=0.0001) and inversely associated with radiographic severity. We observed a positive correlation with the levels of SSAO in the synovial fluid and serum of OA patients (r=0.47; p=0.014). However, serum SSAO levels in OA patients were lower than in controls, and inversely correlated with pain and inflammation markers (CRP and soluble RAGE). Serum SSAO levels were also lower in radiographically severe (KL4) OA patients compared to KL2/3. Serum SSAO did not correlate with other markers of inflammation or radiographic joint space narrowing (JSN) over 24 months.

Conclusion: Synovial fluid VAP-1/SSAO levels were elevated in OA and correlate with radiographic severity. However, serum or circulating SSAO levels are lower in OA patients and inversely correlate with pain and inflammation. Serum VAP-1 levels could identify patients at increased risk for knee radiographic severity.

Disclosure: E. Bournazou, None; J. Samuels, None; H. Zhou, None; S. Krasnokutsky Samuels, None; J. Patel, None; J. Bencardino, None; L. Rybak, None; S. Abamson, None; U. Junker, None; K. Brown, None; M. Attur, None.

To cite this abstract in AMA style:

Bournazou E, Samuels J, Zhou H, Krasnokutsky Samuels S, Patel J, Bencardino J, Rybak L, Abamson S, Junker U, Brown K, Attur M. Vascular Adhesion Protein-1 (VAP-1) As Predictor of Radiographic Severity in Symptomatic Knee Osteoarthritis [abstract]. Arthritis Rheumatol. 2018; 70 (suppl 9). https://acrabstracts.org/abstract/vascular-adhesion-protein-1-vap-1-as-predictor-of-radiographic-severity-in-symptomatic-knee-osteoarthritis/. Accessed .

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