Session Type: ACR Poster Session A
Session Time: 9:00AM-11:00AM
Methods: The amount of ccfDNA was measured by qPCR in peripheral bloods from 29 patients with RA, and 21 healthy controls., and in synovial fluid of knee joint from 13 patients with RA, and 12 patients with OA. By using human synovial cells, culture-supernatants were collected in each steps of cellular-confluency; 40% to 120%. In addition, after stimulated with interleukin(IL)6/soluble IL6 receptor(sIL6R) or TNFα, synovial cells were further treated with and without tocilizumab(TCZ: 100μg/mL) or etanercept(ETN: 10μg/mL) to collect supernatants. When examining ccfDNA in supernatants by qPCR, we amplified both short and long DNA fragments using ALU115-bp primer and ALU247-bp primer to distinguish apoptotic fragments from non-apoptotic fragments, respectively.
To cite this abstract in AMA style:Hashimoto N, Yoshida K, Hashimoto T, Nakai A, Kaneshiro K, Suzuki K, Kawasaki Y, Shibanuma N, Nakagawa N, Sakai Y, Hashiramoto A. TCZ Modulates the Production of Ccfdna Derived from RA Synovial Cells [abstract]. Arthritis Rheumatol. 2016; 68 (suppl 10). https://acrabstracts.org/abstract/tcz-modulates-the-production-of-ccfdna-derived-from-ra-synovial-cells/. Accessed November 29, 2020.
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