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Abstract Number: 0071

Stage-Specific Roles of Interleukin-23/Interleukin-17 Axis and Type 1 Regulatory T Cells Dynamics in Axial Spondyloarthritis

Jina Yeo1, Min-Gang Kim2, Hee Sung Kwon3, Mi Ryoung Seo1, Hyo-Jin Choi1, YunJae Jung4, Eun Young Lee5 and Han Joo Baek6, 1Division of Rheumatology, Department of Internal Medicine, Gil Medical Center, Gachon University College of Medicine, Incheon, Republic of Korea, 2Department of Biomedical Sciences, Seoul National University College of Medicine, Seoul, Republic of Korea, 3Division of Rheumatology, Department of Internal Medicine, Seoul National University Hospital, Seoul, Republic of Korea, 4Department of Microbiology, College of Medicine, Gachon University, Incheon, Republic of Korea, 53Division of Rheumatology, Department of Internal Medicine, Seoul National University Hospital, Seoul, Republic of Korea, 6Division of Rheumatology, Department of Internal Medicine, Gil Medical Center, Gachon University College of Medicine, Inchon, South Africa

Meeting: ACR Convergence 2024

Keywords: , , ,

Session Information

Date: Saturday, November 16, 2024

Title: SpA Including PsA – Basic Science Poster

Session Type: Poster Session A

Session Time: 10:30AM-12:30PM

Background/Purpose: The interleukin (IL)-23/IL-17 pathway is central to axial spondyloarthritis (axSpA) pathogenesis, yet treatments targeting IL-23 show inconsistent effectiveness across spondyloarthritis subtypes. We hypothesized that the IL-23/IL-17 axis varies with disease stage and is influenced by regulatory counterparts, such as type 1 regulatory T (Tr1) cells.

Methods: We assessed the levels of IL-17A and IL-23 in serum and the fraction of CD4+IL-17A+ cells, monocytes (CD14+HLR-DR+), DCs (CD11c+HLA-DR+), and type 1 regulatory T cells (Tr1, CD4+CD49b+LAG3+FoxP3–IL-10+) in peripheral blood obtained from patients with axSpA using enzyme-linked immunosorbent assay and flow cytometry. We also assessed the production of IL-17A from CD4+ T cells from healthy controls following co-culture with monocyte-derived DCs (moDCs) from patients with nr-axSpA compared with those from patients with r-axSpA.

Results: Blood samples from 20 nr-axSpA and 24 r-axSpA patients were analyzed. Serum IL-23 was significantly higher in nr-axSpA patients (p = 0.014), while IL-17A was similar in both groups (p = 0.792). Monocytes and DCs from nr-axSpA produced more IL-23 (p = 0.028 for monocyte, and p = 0.010 for DC), but there were no significant differences in Th17 cells between the groups (p > 0.05). moDCs from nr-axSpA patients stimulated higher proinflammatory cytokine production in CD4+ T cells from healthy controls compared to those from r-axSpA (p < 0.05). Notably, Tr1 cells were significantly lower in nr-axSpA (p = 0.025), and IL-10 treatment reduced IL-23 production by moDCs from nr-axSpA but not from r-axSpA.

Conclusion: IL-23 might play a pathogenic role only in the early disease of axSpA, and IL-17 might be dissociated from IL-23 in advanced disease with enhanced IL-10 producing Tr1 cells as a regulatory counterpart, which requires further elucidation.

Disclosures: J. Yeo: None; M. Kim: None; H. Kwon: None; M. Seo: None; H. Choi: None; Y. Jung: None; E. Lee: None; H. Baek: None.

To cite this abstract in AMA style:

Yeo J, Kim M, Kwon H, Seo M, Choi H, Jung Y, Lee E, Baek H. Stage-Specific Roles of Interleukin-23/Interleukin-17 Axis and Type 1 Regulatory T Cells Dynamics in Axial Spondyloarthritis [abstract]. Arthritis Rheumatol. 2024; 76 (suppl 9). https://acrabstracts.org/abstract/stage-specific-roles-of-interleukin-23-interleukin-17-axis-and-type-1-regulatory-t-cells-dynamics-in-axial-spondyloarthritis/. Accessed .

ACR Meeting Abstracts - https://acrabstracts.org/abstract/stage-specific-roles-of-interleukin-23-interleukin-17-axis-and-type-1-regulatory-t-cells-dynamics-in-axial-spondyloarthritis/