ACR Meeting Abstracts

ACR Meeting Abstracts

  • Meetings
    • ACR Convergence 2024
    • ACR Convergence 2023
    • 2023 ACR/ARP PRSYM
    • ACR Convergence 2022
    • ACR Convergence 2021
    • ACR Convergence 2020
    • 2020 ACR/ARP PRSYM
    • 2019 ACR/ARP Annual Meeting
    • 2018-2009 Meetings
    • Download Abstracts
  • Keyword Index
  • Advanced Search
  • Your Favorites
    • Favorites
    • Login
    • View and print all favorites
    • Clear all your favorites
  • ACR Meetings

Abstract Number: 0879

Recommendations for a Standardized Approach to Histopathologic Evaluation of Synovial Membrane in Murine Models of Experimental Osteoarthritis

Alia Obeidat1, Sung Yeon Kim2, Baofeng Hu3, Jun Li1, Shingo Ishihara1, Rui Xiao4, Rachel Miller1, Anne-Marie Malfait1 and Carla Scanzello5, 1Rush University Medical Center, Chicago, IL, 2University of Pennsylvania School of Engineering and Applied Sciences, Philadelphia, PA, 3University of Pennsylvania, Wynnewood, PA, 4Department of Biostatistics, Epidemiology and Informatics, Perelman School of Medicine, University of Pennsylvania,Department of Pediatrics Division of Biostatistics, Children's Hospital of Philadelphia, Philadelphia, PA, 5Translational Musculoskeletal Research Center, Corp. Michael J Crescenz VA Medical Center,4Division of Rheumatology, Perelman School of Medicine University of Pennsylvania, Philadelphia, PA

Meeting: ACR Convergence 2023

Keywords: Mouse, Osteoarthritis, Synovitis

  • Tweet
  • Click to email a link to a friend (Opens in new window) Email
  • Click to print (Opens in new window) Print
Session Information

Date: Monday, November 13, 2023

Title: (0859–0885) Osteoarthritis & Joint Biology – Basic Science Poster

Session Type: Poster Session B

Session Time: 9:00AM-11:00AM

Background/Purpose: Synovial pathology is an active component of osteoarthritis (OA) that has been linked to disease progression and pain in patients. Macroscopic and microscopic grading systems for synovial pathology in human OA have been extensively described. However, a comprehensive histologic scoring system for murine models of OA is lacking. Thus, we sought to develop a reproducible approach and set of recommendations for evaluation of synovial changes in murine models of OA.

Methods: Coronal sections from murine knee joints as part of other studies were used as follows: 1. Hematoxylin and Eosin (H&E) or Toluidine Blue (T-Blue) stained sections from mice 6 weeks after destabilization of medial meniscus (DMM) or sham surgery and age matched naïve controls (n=5/group), 2. Safranin O (Saf-O) sections from mice 16 weeks after DMM or sham surgery (n=5-6/group), 3. T-Blue sections from mice 16 weeks after DMM or sham surgery (n=10/group), 4. T-Blue sections from 12 weeks after partial meniscectomy (PMX) or sham surgery (n=10/group), 5. H&E sections from 12 weeks after PMX or sham surgery (n=5/group). Four blinded readers graded four pathological features (hyperplasia, cellularity, inflammation, and fibrosis) at four anatomic locations (lateral and medial femoral gutters, and lateral and medial tibial gutters) using midjoint sections. Inter-reader reliability tests (IRR) (Kendall’s coefficient of concordance (W) for hyperplasia, inflammation, and cellularity; Fleiss Kappa test (κ) for fibrosis) were performed to assess agreement between readers.

Results: IRR measures (Table 1) demonstrate that there was acceptable to very good agreement between raters. Inflammation scores were excluded because of poorer concordance between readers and overlap with the cellularity assessment. Increased hyperplasia and cellularity and a trend of increased fibrosis were observed in the medial tibial and medial femoral gutters compared to controls 6wks after DMM (Fig 1A-C). At 16-weeks post-DMM, significant increase in cellularity and fibrosis were observed in the medial side with no significant hyperplasia (Fig 1D-F). No significant lateral changes were observed in any of DMM groups. At 12 weeks after PMX, a significant increase in hyperplasia, cellularity and fibrosis were observed in the medial gutters compared to sham mice (Fig 1G-I). Increased cellularity and hyperplasia were evident in the lateral gutters of PMX mice. Additionally, we assessed 4 sections/knee from PMX mice and found that synovial changes were consistent from section to section (Fig 2). Finally, we assessed the effect of different stains on IRR (Table 1). Higher reliability was observed using H&E and T-blue stains.

Conclusion: Based on our findings, we suggest evaluating 3 pathological features at 4 anatomic areas of the joint to assess synovial pathology in murine models of OA. A minimum of 3 readers are recommended, for which the IRR should be determined. A single midjoint section is likely sufficient for analysis, and a careful choice of stain should be considered. It is important to note that these recommendations are considered an initial step to allow comparison across labs and models, and to be used to guide more detailed subsequent analyses.

Supporting image 1

Supporting image 2

Supporting image 3


Disclosures: A. Obeidat: None; S. Kim: None; B. Hu: None; J. Li: None; S. Ishihara: None; R. Xiao: None; R. Miller: None; A. Malfait: None; C. Scanzello: None.

To cite this abstract in AMA style:

Obeidat A, Kim S, Hu B, Li J, Ishihara S, Xiao R, Miller R, Malfait A, Scanzello C. Recommendations for a Standardized Approach to Histopathologic Evaluation of Synovial Membrane in Murine Models of Experimental Osteoarthritis [abstract]. Arthritis Rheumatol. 2023; 75 (suppl 9). https://acrabstracts.org/abstract/recommendations-for-a-standardized-approach-to-histopathologic-evaluation-of-synovial-membrane-in-murine-models-of-experimental-osteoarthritis/. Accessed .
  • Tweet
  • Click to email a link to a friend (Opens in new window) Email
  • Click to print (Opens in new window) Print

« Back to ACR Convergence 2023

ACR Meeting Abstracts - https://acrabstracts.org/abstract/recommendations-for-a-standardized-approach-to-histopathologic-evaluation-of-synovial-membrane-in-murine-models-of-experimental-osteoarthritis/

Advanced Search

Your Favorites

You can save and print a list of your favorite abstracts during your browser session by clicking the “Favorite” button at the bottom of any abstract. View your favorites »

All abstracts accepted to ACR Convergence are under media embargo once the ACR has notified presenters of their abstract’s acceptance. They may be presented at other meetings or published as manuscripts after this time but should not be discussed in non-scholarly venues or outlets. The following embargo policies are strictly enforced by the ACR.

Accepted abstracts are made available to the public online in advance of the meeting and are published in a special online supplement of our scientific journal, Arthritis & Rheumatology. Information contained in those abstracts may not be released until the abstracts appear online. In an exception to the media embargo, academic institutions, private organizations, and companies with products whose value may be influenced by information contained in an abstract may issue a press release to coincide with the availability of an ACR abstract on the ACR website. However, the ACR continues to require that information that goes beyond that contained in the abstract (e.g., discussion of the abstract done as part of editorial news coverage) is under media embargo until 10:00 AM ET on November 14, 2024. Journalists with access to embargoed information cannot release articles or editorial news coverage before this time. Editorial news coverage is considered original articles/videos developed by employed journalists to report facts, commentary, and subject matter expert quotes in a narrative form using a variety of sources (e.g., research, announcements, press releases, events, etc.).

Violation of this policy may result in the abstract being withdrawn from the meeting and other measures deemed appropriate. Authors are responsible for notifying colleagues, institutions, communications firms, and all other stakeholders related to the development or promotion of the abstract about this policy. If you have questions about the ACR abstract embargo policy, please contact ACR abstracts staff at [email protected].

Wiley

  • Online Journal
  • Privacy Policy
  • Permissions Policies
  • Cookie Preferences

© Copyright 2025 American College of Rheumatology