Session Type: ACR Poster Session A
Session Time: 9:00AM-11:00AM
Background/Purpose: Extensive evidence has correlated epigenetic alterations in articular tissues with both the presence and progression of human osteoarthritis. A recent study demonstrated that accelerated DNA methylation aging was present in OA cartilage compared to controls but not in peripheral blood samples from a mixed population of knee, hip, and hand OA patients. To further clarify epigenetic age changes specifically in knee OA, we examined the DNA methylation aging rate in peripheral blood mononuclear cells (PBMCs) at baseline from knee OA patients with rapid radiographic progression compared to well-matched nonprogressors enrolled in the Osteoarthritis Initiative (OAI).
Methods: Peripheral blood mononuclear cell (PBMC) DNA was obtained from baseline blood draws of 64 OA patients enrolled in the Osteoarthritis Initiative (OAI) longitudinal study. 32 rapidly-progressive OA patients, defined as ≥1.0mm radiographic joint space loss or joint replacement within the first 24 months of follow-up were compared to 32 non-progressive OA patients defined as ≤0.5mm radiographic joint space loss over 48 months of follow-up. There were no differences in age, sex, race, BMI, baseline K/L grade, or calculated PBMC subset composition between rapid- and non-progressors. DNA methylation was quantified with Illumina HumanMethylation 450k arrays. Epigenetic age was estimated with the algorithm described by Horvath et. al., using 353 age-associated CpG sites. This epigenetic age was compared to chronological age to calculate epigenetic-chronological age discordance (ΔAge) and group differences compared with a Student t-test. ΔAge was correlated with individual CpG methylation sites of rapid progressors, and Pearson values calculated. Correlation was considered significant if Pearson’s r values were ≤-0.55 or ≥0.55 (p≤0.001). Pathway analysis of correlated genes was performed with the Ingenuity Pathway Analysis (IPA) system.
Results: The baseline DNA methylation aging rate in rapidly progressive (RP) knee OA patients was decelerated compared to nonprogressors (NP) and to chronological age (ΔAge-RP: -4.9±1.4 vs. ΔAge-NP: -0.071±1.3 mean±SEM years less than chronological age, p=0.015). 1165 CpG sites were correlated with ΔAge in rapid progressors, corresponding to 755 genes. Ontologic analysis of highly correlated genes showed association of the STAT3 pathway (p=6E-4), Notch signaling (p=1E-3), axonal guidance signaling (p=7E-3), CREB signaling (p=2E-2), NFAT signaling (p=2E-2), and autophagy (p=4E-2) among others. Associated upstream regulators included FGF2 (p=3E-5), SMAD4 (p=9E-4), SMAD5 (p=1E-3, TNF (p=4E-3), and TGFB1 (p=4E-3), among others.
Conclusion: Our data reveal that a decelerated peripheral blood differential DNA methylation age epigenotype is present at baseline in rapidly progressive knee OA patients, but not in nonprogressive knee OA patients. The genes correlated with this methylation age deceleration cluster in pathways previously associated with OA in articular tissues. Our data reinforce the notion that OA is a heterogeneous disease composed of distinct subgroups, and suggests that future epigenetic investigation of immune cell subsets may be beneficial in unraveling OA pathogenesis.
To cite this abstract in AMA style:Rivas A, Andrews M, Jeffries MA. Genome-Wide DNA Methylation Profiling of OA PBMCs Reveals Slowed Epigenetic Aging Among Rapid Radiographic Progressors: Data from the Osteoarthritis Initiative (OAI) [abstract]. Arthritis Rheumatol. 2017; 69 (suppl 10). https://acrabstracts.org/abstract/genome-wide-dna-methylation-profiling-of-oa-pbmcs-reveals-slowed-epigenetic-aging-among-rapid-radiographic-progressors-data-from-the-osteoarthritis-initiative-oai/. Accessed February 28, 2020.
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ACR Meeting Abstracts - https://acrabstracts.org/abstract/genome-wide-dna-methylation-profiling-of-oa-pbmcs-reveals-slowed-epigenetic-aging-among-rapid-radiographic-progressors-data-from-the-osteoarthritis-initiative-oai/