Session Type: ACR Poster Session A
Session Time: 9:00AM-11:00AM
Background/Purpose: Impaired angiogenesis in SSc is a crucial component of disease pathology that occurs in spite of upregulation of VEGF and other proangiogenic factors. MicroRNA-126 (miR-126) is expressed mainly in MVECs throughout the capillaries. MiR-126 regulates angiogenic signaling and responses to VEGF by direct repression of negative regulators of VEGF signalling pathway, including the Sprouty-related protein-1 (Spred1), and phosphoinositol-3 kinase regulatory subunit 2 (PIK3R2). In this study we investigated the expression levels of miR-126 and VEGF responses in SSc and control MVECs.
Methods: <span’arial’,’sans-serif’;”>MVECs were isolated from involved SSc skin and matched healthy subjects. The expression of miR-126 was measured by qPCR. The expression levels of VEGF inhibitors Spred1 and PIK3R2 were examined by qPCR and western analysis. The angiogenic potentials of MVECs were tested in a matrigel-based tube formation assay and in scratch test migration assay. <span’arial’,’sans-serif’;”>The expression of miR-126 was inhibited in control-MVECs by transfecting cells with has-miR-126 inhibitor and enhanced in SSc-MVECs by transfecting cells with has-miR-126 Mimic.
To cite this abstract in AMA style:kahaleh B, Altorok N, Wang Y, Nada S, Madkhali M, Sun J. Down-Regulation of microRNA-126 in Scleroderma Microvascular Endothelial Cells (MVECs) Is Associated with Impaired Responses to Vascular Endothelial Growth Factor (VEGF) and Defective Angiogenesis [abstract]. Arthritis Rheumatol. 2016; 68 (suppl 10). https://acrabstracts.org/abstract/down-regulation-of-microrna-126-in-scleroderma-microvascular-endothelial-cells-mvecs-is-associated-with-impaired-responses-to-vascular-endothelial-growth-factor-vegf-and-defective-angiogenesi/. Accessed August 4, 2021.
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