Session Information
Session Type: Abstract Session
Session Time: 2:00PM-3:30PM
Background/Purpose: Rheumatoid arthritis (RA) is an autoimmune disease characterized by immune infiltration of the synovial tissue lining joints. RA is clinically heterogenous, with patients having varied responses to therapies, likely reflecting distinct pathogenic mechanisms. To begin to define the heterogeneity in RA, a recent study showed that the fractional abundances of 5 cell types can help predict treatment response in some subsets of RA (1). With the goal of better understanding the spatial organization of cells in RA synovial tissues and mapping cell-cell interactions, we sought to take a key next step in defining RA heterogeneity and its relation to treatment response
Methods:
Spatial transcriptomic profiling
We used CosMx Spatial Molecular Imager to measure the RNA expression levels of 960 genes in 10 paraffin-embedded inflamed synovial tissue samples.
Cell segmentation and annotation
We developed a segmentation pipeline to map transcripts to cells, and then classified each cell into one of 10 major cell types. We used the single-cell RNA seq synovial atlas to map these cells further into fine-grained cell-states.
Niche detection and characterization
We performed spatial tissue segmentation to identify contiguous and transcriptionally homogeneous sections of the tissue called “niches”. We split tissues into tiles and performed spatial clustering of the tiles to detect niches.
Results:
1. Identification of major and fine-grain cell types in situ. We segmented and labeled 333,966 high quality cells across 10 RA synovial tissue samples. We identified 10 major cell lineages (Figure 1A-B), including 2 absent in single-cell RNA-seq studies: mast cells and neutrophils. We subclassified cells into 54 subtypes and activation states based on prior definitions from the AMP single-cell RNA-seq atlas of inflamed synovium (1). We detected 2 cell states of mast cells (resting and activated) and 3 cell states of neutrophils (resting, activated, and proliferating) based on key gene expression markers.
2. Quantification of anatomic niches. We mapped annotated cells into tissue space, where they organized into anatomic structures: vasculature (endothelial and mural cells), lining (lining fibroblasts and MERTK+ macrophages), and immune aggregates (T, B, and plasma cells) (Figure 2A-C). We quantified the boundaries of these niches and identified all niches in most samples. We next quantified the relative composition of niches within and across donors and observed significant heterogeneity of niches within samples (Figure 2D-F).
3. Validation with independent cohort. We validated our spatial organization results in an independent inflamed synovial tissue cohort from 3 patients with treatment naïve RA. Using our pipeline, we found similar proportions of cell types and organization of cells into the canonical niches defined in our spatial atlas (Figure 3A, B).
Conclusion: We established a draft spatial atlas of RA synovium and provided spatial context for 54 cell types, subtypes, and activation states observed in dissociated tissue assays. This atlas will serve as a reference to map the cellular organization in different cohorts as demonstrated and eventually enable us to compare the effects of different therapeutics on the RA tissue.
To cite this abstract in AMA style:
Madhu R, Bhamidipati K, Jonsson A, Zhang F, MacDonald L, Kim Y, Cui Y, BWH S, RA SLE Network A, Kurowska-Stolarska M, Alvernini S, Gravallese E, Brenner M, Raychaudhuri S, Wei K, Korsunsky i. Characterizing Spatial Organization of Immune Infiltrates in Rheumatoid Arthritis Synovia Using Spatial Transcriptomic Analysis [abstract]. Arthritis Rheumatol. 2023; 75 (suppl 9). https://acrabstracts.org/abstract/characterizing-spatial-organization-of-immune-infiltrates-in-rheumatoid-arthritis-synovia-using-spatial-transcriptomic-analysis/. Accessed .« Back to ACR Convergence 2023
ACR Meeting Abstracts - https://acrabstracts.org/abstract/characterizing-spatial-organization-of-immune-infiltrates-in-rheumatoid-arthritis-synovia-using-spatial-transcriptomic-analysis/