ACR Meeting Abstracts

ACR Meeting Abstracts

Abstract Number: 2868

NK Cell Characterization in Patients with Systemic Lupus Erythematosus: Increased Frequency of Ki67+ NK Cells Associated with Disease Activity and Type I Interferon Signature

Kelly Hudspeth1, Shu Wang2, Jingya Wang2, Saifur Rahman2, Michael Smith2, Kerry Casey2, Geoffrey Stephens3, Miguel Sanjuan2, Autoimmunity Molecular Medicine group2, Zerai G. Manna4, Sarfaraz Hasni4, Rachel Ettinger5 and Richard Siegel6, 1Immunoregulation Section, Autoimmunity Branch, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD, 2Respiratory, Inflammation, and Autoimmunity Group, MedImmune LLC, Gaithersburg, MD, 3Respiratory, Inflammatory, and Autoimmune Diseases Research, Medimmune, LLC, Gaithersburg, MD, 4National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD, 5Respiratory, Inflammation and Autoimmunity (RIA), MedImmune, LLC, Gaithersburg, MD, 6Immunoregulation Section, Autoimmunity Branch and Office of the Clinical Director National Institute of Arthritis and Musculoskeletal and Skin Diseases, NIH, Bethesda, MD

Meeting: 2016 ACR/ARHP Annual Meeting

Date of first publication: September 28, 2016

Keywords: , ,

Session Information

Date: Tuesday, November 15, 2016

Title: Systemic Lupus Erythematosus – Human Etiology and Pathogenesis - Poster II

Session Type: ACR Poster Session C

Session Time: 9:00AM-11:00AM

Background/Purpose: Systemic Lupus Erythematosus (SLE) is a complex autoimmune disorder whose pathology appears to involve many immune cell types. While it is clear that autoantibody producing B cells as well as CD4+ T cell help are key contributors to disease, little is known regarding the role of innate lymphoid cells such as Natural Killer (NK) cells in the pathogenesis of SLE.

Methods: We have characterized the phenotype of NK cells by multicolor flow cytometry, proteomics and genomics arrays in a large cohort of patients with SLE.

Results: While the overall percentage of NK cells was similar or slightly decreased compared to healthy controls, a subset of patients displayed a high frequency of NK cells expressing the proliferation marker, Ki-67, which was not found in healthy donors. Only a moderate increase of Ki-67 was observed on other immune cell types such as total CD4+, CD8+ T cells or CD19+ B cells in the same donors. Increased NK cell expression of Ki-67 was found to correlate with clinical parameters. Interestingly, elevated frequencies of Ki67+ NK cells were highly associated with active nephritis. Proteomics analysis and auto-antibody arrays also revealed significant correlations between NK cell expression of Ki-67 and type I interferon (IFN) gene score as well as SLE associated auto-antibodies

Conclusion: These results will contribute to the understanding of the mechanistic role of NK cells in immune-mediated pathology of SLE.

Disclosure: K. Hudspeth, None; S. Wang, Medimmune, LLC, 1; J. Wang, MedImmune, LLC, 1; S. Rahman, MedImmune, LLC, 1; M. Smith, MedImmune, LLC, 1; K. Casey, MedImmune, LLC, 1; G. Stephens, AstraZeneca, 1; M. Sanjuan, MedImmune, LLC, 1; A. M. Medicine group, MedImmune, LLC, 1; Z. G. Manna, None; S. Hasni, None; R. Ettinger, MedImmune, LLC, 1; R. Siegel, None.

To cite this abstract in AMA style:

Hudspeth K, Wang S, Wang J, Rahman S, Smith M, Casey K, Stephens G, Sanjuan M, Medicine group AM, Manna ZG, Hasni S, Ettinger R, Siegel R. NK Cell Characterization in Patients with Systemic Lupus Erythematosus: Increased Frequency of Ki67+ NK Cells Associated with Disease Activity and Type I Interferon Signature [abstract]. Arthritis Rheumatol. 2016; 68 (suppl 10). https://acrabstracts.org/abstract/nk-cell-characterization-in-patients-with-systemic-lupus-erythematosus-increased-frequency-of-ki67-nk-cells-associated-with-disease-activity-and-type-i-interferon-signature/. Accessed .

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