Abstracts tagged "Neutrophil Extracellular Traps"

Abstract Number: 2058 • 2016 ACR/ARHP Annual Meeting
Ectonucleotidase-Mediated Protection of Lupus Mice from Exaggerated Immune Responses and Arterial Vasculopathy
Jason S Knight¹, Levi F Mazza¹, Srilakshmi Yalavarthi¹, Yogen Kanthi² and David J Pinsky², ¹Division of Rheumatology, University of Michigan, Ann Arbor, MI, ²Division of Cardiovascular Medicine, University of Michigan, Ann Arbor, MI
Background/Purpose: CD39 and CD73 are so-called ectonucleotidases, surface enzymes expressed by leukocytes and endothelial cells that jut into the extracellular space. There, they mediate the…
Abstract Number: 3017 • 2016 ACR/ARHP Annual Meeting
Elevated Neutrophil Extracellular Trap Levels Correlate with Anti-CCP3-IgG and Anti-CCP3-IgA Levels in the Sputum of Individuals at-Risk for Future Rheumatoid Arthritis
M. Kristen Demoruelle¹, Monica Purmalek², Heather Rothfuss³, Michael Weisman⁴, Lindsay Kelmenson¹, Michael Mahler⁵, Jill M. Norris⁶, Brian Cherrington³, Mariana Kaplan², V. Michael Holers¹ and Kevin D. Deane¹, ¹Rheumatology Division, University of Colorado Denver, Aurora, CO, ²Systemic Autoimmunity Branch, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD, ³Zoology and Physiology, University of Wyoming, Laramie, WY, ⁴Rheumatology, Cedars-Sinai Medical Center, Los Angeles, CA, ⁵Research and Development, Inova Diagnostics, San Diego, CA, ⁶Epidemiology, Colorado School of Public Health, Aurora, CO
Background/Purpose: The initial site of anti-citrullinated protein antibody (ACPA) generation in RA has been proposed to be a mucosal site. We have previously demonstrated ACPA…
Abstract Number: 573 • 2016 ACR/ARHP Annual Meeting
Synovial Fibroblast-Neutrophil Interactions Promote Pathogenic Adaptive Immunity in Rheumatoid Arthritis
Carmelo Carmona-Rivera¹, Erica Moore², Nithya Lingampalli³, Hannes Uchtenhagen⁴, Eddie James⁵, Kevin L. Bicker⁶, Heidi Wähämaa⁷, Victoria Hoffmann⁸, Anca I Catrina⁷, Paul Thompson⁹, Jane H. Buckner⁵, William Robinson¹⁰, David Fox¹¹ and Mariana Kaplan², ¹Systemic Autoimmunity Branch/ NIAMS, National Institutes of Health, Bethesda, MD, ²Systemic Autoimmunity Branch, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD, ³Division of Immunology and Rheumatology, Stanford University School of Medicine, Stanford, CA, ⁴Translational Research Program, Benaroya Research Institute at Virginia Mason, Seattle, WA, ⁵Benaroya Research Institute at Virginia Mason, Seattle, WA, ⁶Middle Tennessee State University, Murfreesboro, TN, ⁷Rheumatology Unit, Department of Medicine, Karolinska Institute, Karolinska University Hospital, Stockholm, Sweden, ⁸Division of Veterinary Resources, National Institutes of Health, Bethesda, MD, ⁹University of Massachusetts, Worcester, MA, ¹⁰Stanford University School of Medicine, Stanford, CA, ¹¹Department of Medicine [Division of Rheumatology], University of Michigan, Ann Arbor, MI
Background/Purpose: Methods: Results: Conclusion:
Abstract Number: 1006 • 2016 ACR/ARHP Annual Meeting
Immune Complex-Mediated TLR8 Activation Shifts Neutrophils from Phagocytosis to Netosis through Furin-Dependent Shedding of Fcgriia
Christian Lood¹, Sabine Arve², Laura Durcan², Jeffrey Ledbetter² and Keith B. Elkon¹, ¹Department of Medicine, Division of Rheumatology, University of Washington, Seattle, WA, ²University of Washington, Seattle, WA
Background/Purpose: Neutrophils, isolated from healthy individuals, were incubated with RNA-ICs and analyzed for phagocytosis and NETosis by flow cytometry and fluorimetry, respectively, in the presence…
Abstract Number: 1756 • 2016 ACR/ARHP Annual Meeting
Novel Role of Rho Kinase in Neutrophil Netosis during UVB Induced-Skin Inflammation
Ming-Lin Liu^1,2, Meena Sharma^2,3 and Victoria P. Werth^4,5, ¹Department of Dermatology,, Philadelphia V.A. Medical Center, Philadelphia, PA, ²Department of Dermatology, University of Pennsylvania, Philadelphia, PA, ³Department of Dermatology, Philadelphia V.A. Medical Center, Philadelphia, PA, ⁴Dermatology, University of Pennsylvania, Philadelphia, PA, ⁵Philadelphia V.A. Medical Center, Philidelphia, PA
Background/Purpose: Skin inflammation appears in cutaneous or systemic lupus erythematosus. Ultraviolet B (UVB) is an important environmental trigger of skin inflammation in lupus. Mechanisms linking…
Abstract Number: 1962 • 2016 ACR/ARHP Annual Meeting
Antiphospholipid Syndrome Neutrophils Are Characterized By Overexpression of P-Selectin Glycoprotein Ligand 1, a Potential Therapeutic Target
Jason S Knight¹, Patrick S. Coit¹, He Meng¹, Srilakshmi Yalavarthi¹, Paul Renauer¹, Robert C Grenn¹, Levi F Mazza¹, Hui Wang², Daniel T Eitzman² and Amr H Sawalha¹, ¹Division of Rheumatology, University of Michigan, Ann Arbor, MI, ²Division of Cardiovascular Medicine, University of Michigan, Ann Arbor, MI
Background/Purpose: Treatment of the thrombotic manifestations of antiphospholipid syndrome (APS) primarily focuses on inhibiting clotting pathways. In an effort to identify upstream inflammatory targets that…
Abstract Number: 2073 • 2015 ACR/ARHP Annual Meeting
Mitochondrial ROS Is a Novel Regulator of Sting-Mediated Type I IFN Production By Governing Extrusion of Oxidized Mitochondrial DNA upon Neutrophil Extracellular Trap Formation.
Christian Lood¹, Luz P. Blanco², Monica Purmalek³, Carolyne K. Smith³, Carmelo Carmona-Rivera³, Jeffrey Ledbetter⁴, Mariana J. Kaplan² and Keith B. Elkon⁵, ¹Department of Medicine, Division of Rheumatology, University of Washington, Seattle, WA, ²Systemic Autoimmunity Branch, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD, ³Systemic Autoimmunity Branch, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD, ⁴University of Washington, Seattle, WA, ⁵Division of Rheumatology, University of Washington, Seattle, WA
Background/Purpose: Neutrophil extracellular trap generation (NETosis) is a reactive oxygen species (ROS)-dependent cell death pathway implicated in autoimmune disorders such as systemic lupus erythematosus (SLE).…
Abstract Number: 2072 • 2015 ACR/ARHP Annual Meeting
Mitochondrial Reactive Oxygen Species Modulate Autoimmunity in Systemic Lupus Erythematosus
Luz P. Blanco¹, Christian Lood², Monica Purmalek³, Suk See DeRavin⁴, Carolyne K. Smith³, Carmelo Carmona-Rivera³, Harry Malech⁴, Jeffrey Ledbetter⁵, Keith B. Elkon⁶ and Mariana J. Kaplan¹, ¹Systemic Autoimmunity Branch, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD, ²Internal Medicine, University of Washington, Seattle, WA, ³Systemic Autoimmunity Branch, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD, ⁴NIAID/NIH, Bethesda, MD, ⁵University of Washington, Seattle, WA, ⁶Department of Medicine & Immunology, University of Washington, Seattle, WA
Background/Purpose: Dysregulation in the formation of neutrophil extracellular traps (NETs) may contribute to modification and externalization of autoantigens and to organ damage in diverse autoimmune…
Abstract Number: 2002 • 2015 ACR/ARHP Annual Meeting
Dnase Treatment Protects Against DVT Formation in a Mouse Model of Antiphospholipid Syndrome
He Meng¹, Srilakshmi Yalavarthi², Levi F. Mazza³, Megan A. Elfline¹, Catherine E. Luke¹, Peter K. Henke¹ and Jason S. Knight², ¹University of Michigan, Ann Arbor, MI, ²Division of Rheumatology, University of Michigan, Ann Arbor, MI, ³Department of Internal Medicine, Division of Rheumatology, University of Michigan, Ann Arbor, MI
Background/Purpose: Antiphospholipid antibodies, especially those targeting beta-2-glycoprotein I (β2GPI), have been shown to amplify thrombosis in mice. However, most published models have relied on endothelial…
Abstract Number: 1787 • 2015 ACR/ARHP Annual Meeting
Innate Immunity, Arterial Inflammation and Vascular Stiffness in Patients with Systemic Lupus Erythematosus
Monica Purmalek¹, Simantini Sakhardande¹, Yenealem Temesgen-Oyelakin², Alice Fike³, Taufiq Salahuddin⁴, Balaji Natarajan⁴, Zerai Manna², Elizabeth Joyal², Sarfaraz Hasni², Nehal N. Mehta⁴ and Mariana J. Kaplan¹, ¹Systemic Autoimmunity Branch, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD, ²National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD, ³Office of the Clinical Director, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD, ⁴NHLBI, National Institutes of Health, Bethesda, MD
Background/Purpose: Patients with systemic lupus erythematosus (SLE) show a striking increase in risk of atherosclerotic cardiovascular disease (CVD) not explained by Framingham risk, compared to…
Abstract Number: 1769 • 2015 ACR/ARHP Annual Meeting
Role of Topical Administration of Peptidylarginine Deiminase Inhibitors in Murine Lupus
Erica Moore¹, Huw Lewis², Carolyne K. Smith¹, Venkataraman Subramaniam³, Victoria Hoffmann⁴, Paul Thompson⁵ and Mariana J. Kaplan¹, ¹Systemic Autoimmunity Branch, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD, ²Immuno Inflammation, GSK, Stevenage, United Kingdom, ³University of Massachusetts, Boston, MA, ⁴ORS, National Institutes of Health, Bethesda, MD, ⁵University of Massachusetts, Worcester, MA
Background/Purpose: Peptidylarginine deiminases (PADs), specifically PAD4, have recently been implicated in the pathogenesis of systemic lupus erythematosus (SLE) through their role in citrullinating histones in…
Abstract Number: 1763 • 2015 ACR/ARHP Annual Meeting
Neutrophil Netosis Formation during the UVB Induced-Skin Inflammation
Ming-Lin Liu^1,2, Meena Sharma^1,3, Shwetapadma Sahu^4,5 and Victoria Werth^4,6, ¹Department of Dermatology, University of Pennsylvania, Philadelphia, PA, ²Department of Dermatology,, Philadelphia V.A. Medical Center, Philadelphia, PA, ³Department of Dermatology, Philadelphia V.A. Medical Center, Philadelphia, PA, ⁴Dermatology, University of Pennsylvania, Philadelphia, PA, ⁵Philadelphia V.A. Medical Center, Philadelphia, PA, ⁶Dermatology, Philadelphia V.A. Medical Center, Philadelphia, PA
Background/Purpose: Excessive exposure to sunlight, particularly ultraviolet B (UVB), induces autoimmune skin inflammation and other skin diseases. Although UVB mainly causes damage to the epidermis,…
Abstract Number: 1085 • 2015 ACR/ARHP Annual Meeting
Levamisole Triggers Neutrophil Extracellular Trap Formation through Muscarinic Receptors in Patients with Drug-Induced Vasculitis
Peter C. Grayson¹, Carmelo Carmona-Rivera², Meryl Waldman³, Karran Phillips⁴, Venkataraman Subramaniam⁵, Paul Thompson⁶, Jonathan D. Graf⁷ and Mariana J. Kaplan¹, ¹National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD, ²Systemic Autoimmunity Branch/ NIAMS, National Institutes of Health, Bethesda, MD, ³NIDDK, National Institutes of Health, Bethesda, MD, ⁴NIDA, National Institutes of Health, Baltimore, MD, ⁵Biochemistry and Molecular Pharmacology, University of Massachusetts, Worcester, MA, ⁶Biochemistry and Molecular Pharmacology, University of Masshachussetts, Worcester, MA, ⁷Rosalind Russell / Ephraim P. Engleman Rheumatology Research Center, University of California, San Francisco, San Francisco, CA
Background/Purpose: Levamisole, an anti-helminth drug, has been implicated in cases of drug-induced autoimmunity in humans exposed to adulterated cocaine. Clinical manifestations of levamisole-induced autoimmunity include…
Abstract Number: 964 • 2015 ACR/ARHP Annual Meeting
Neutrophil Extracellular Traps Are Not Only Targets for ACPA-Positive IgG from Rheumatoid Arthritis Patients but Also Directly Trigger Pro- and Anti-Inflammatory Effects Partly Mediated By the C1q Complement Protein
Matthieu Ribon¹, Sarra Seninet¹, Katarzyna Matyja¹, Mireille Sebbag², Cyril Clavel², Julie Mussard¹, Guy Serre², Marie-Christophe Boissier³ and Patrice Decker¹, ¹Inserm UMR 1125, Li2P, University of Paris 13, Sorbonne Paris Cité, Bobigny, France, ²University of Toulouse, UMR 5165 CNRS-1056 Inserm, Toulouse, France, ³Rheumatology Department, Avicenne Hospital, AP-HP, Bobigny, France
Background/Purpose: Activated neutrophils (PMN) form neutrophil extracellular traps (NET). Those structures are expelled chromatin fibers composed of DNA and associated proteins. The process, NETosis, is…
Abstract Number: 933 • 2015 ACR/ARHP Annual Meeting
DEK-Targeting DNA Aptamers As Novel Therapeutics for Inflammatory Arthritis
Nirit Mor-Vaknin¹, Anjan K. Saha², Maureen Legendre¹, Carmelo Carmona-Rivera³, M. Asif Amin⁴, Bradley J. Rabquer⁵, Marta J. Gonzalez-Hernandez⁶, Julie M. Jorns⁷, Srilakshmi Yalavarthi⁸, Smriti Mohan⁹, Dave Pai¹⁰, Kristine Angevine¹¹, Barbara Adams¹², Jason S. Knight⁸, Alisa E. Koch^13,14,15, David Fox¹⁴, Dave Engelke¹⁶, Mariana J. Kaplan¹⁷ and David Markovitz⁴, ¹Infectious Diseases, University of Michigan, Ann Arbor, MI, ²Medical School, University of Michigan, Ann Arbor, MI, ³Systemic Autoimmunity Branch, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD, ⁴Internal Medicine, University of Michigan, Ann Arbor, MI, ⁵Albion College, Ann Arbor, MI, ⁶Graduate Program in Immunology, University of Michigan, Ann Arbor, MI, ⁷Pathology, University of Michigan, Ann Arbor, MI, ⁸Division of Rheumatology, University of Michigan, Ann Arbor, MI, ⁹Pediatric Rheumatology, University of Michigan, Ann Arbor, MI, ¹⁰Scripps Research Institute, San Diego, CT, ¹¹Appistry, Inc, St. Louis, MO, ¹²Pediatric Rheum PTD, Univ of Michigan Hlth System, Ann Arbor, MI, ¹³VA Medical Service, Ann Arbor, MI, ¹⁴Internal Medicine, Division of Rheumatology, University of Michigan Medical Center, Ann Arbor, MI, ¹⁵Internal Medicine, Veteran's Affairs and University of Michigan, Ann Arbor, MI, ¹⁶Biochemistry, University Michigan, Ann Arbor, MI, ¹⁷Systemic Autoimmunity Branch, NIAMS, National Institutes of Health, Bethesda, MD
Background/Purpose: Aptamers are short single stranded DNA or RNA oligonucleotides that are specifically selected to bind and neutralize a wide range of biomedically relevant proteins.…