Abstracts tagged "Neutrophil Extracellular Traps"

Abstract Number: 573 • 2016 ACR/ARHP Annual Meeting
Synovial Fibroblast-Neutrophil Interactions Promote Pathogenic Adaptive Immunity in Rheumatoid Arthritis
Carmelo Carmona-Rivera¹, Erica Moore², Nithya Lingampalli³, Hannes Uchtenhagen⁴, Eddie James⁵, Kevin L. Bicker⁶, Heidi Wähämaa⁷, Victoria Hoffmann⁸, Anca I Catrina⁷, Paul Thompson⁹, Jane H. Buckner⁵, William Robinson¹⁰, David Fox¹¹ and Mariana Kaplan², ¹Systemic Autoimmunity Branch/ NIAMS, National Institutes of Health, Bethesda, MD, ²Systemic Autoimmunity Branch, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD, ³Division of Immunology and Rheumatology, Stanford University School of Medicine, Stanford, CA, ⁴Translational Research Program, Benaroya Research Institute at Virginia Mason, Seattle, WA, ⁵Benaroya Research Institute at Virginia Mason, Seattle, WA, ⁶Middle Tennessee State University, Murfreesboro, TN, ⁷Rheumatology Unit, Department of Medicine, Karolinska Institute, Karolinska University Hospital, Stockholm, Sweden, ⁸Division of Veterinary Resources, National Institutes of Health, Bethesda, MD, ⁹University of Massachusetts, Worcester, MA, ¹⁰Stanford University School of Medicine, Stanford, CA, ¹¹Department of Medicine [Division of Rheumatology], University of Michigan, Ann Arbor, MI
Background/Purpose: Methods: Results: Conclusion:
Abstract Number: 1006 • 2016 ACR/ARHP Annual Meeting
Immune Complex-Mediated TLR8 Activation Shifts Neutrophils from Phagocytosis to Netosis through Furin-Dependent Shedding of Fcgriia
Christian Lood¹, Sabine Arve², Laura Durcan², Jeffrey Ledbetter² and Keith B. Elkon¹, ¹Department of Medicine, Division of Rheumatology, University of Washington, Seattle, WA, ²University of Washington, Seattle, WA
Background/Purpose: Neutrophils, isolated from healthy individuals, were incubated with RNA-ICs and analyzed for phagocytosis and NETosis by flow cytometry and fluorimetry, respectively, in the presence…
Abstract Number: 1756 • 2016 ACR/ARHP Annual Meeting
Novel Role of Rho Kinase in Neutrophil Netosis during UVB Induced-Skin Inflammation
Ming-Lin Liu^1,2, Meena Sharma^2,3 and Victoria P. Werth^4,5, ¹Department of Dermatology,, Philadelphia V.A. Medical Center, Philadelphia, PA, ²Department of Dermatology, University of Pennsylvania, Philadelphia, PA, ³Department of Dermatology, Philadelphia V.A. Medical Center, Philadelphia, PA, ⁴Dermatology, University of Pennsylvania, Philadelphia, PA, ⁵Philadelphia V.A. Medical Center, Philidelphia, PA
Background/Purpose: Skin inflammation appears in cutaneous or systemic lupus erythematosus. Ultraviolet B (UVB) is an important environmental trigger of skin inflammation in lupus. Mechanisms linking…
Abstract Number: 1962 • 2016 ACR/ARHP Annual Meeting
Antiphospholipid Syndrome Neutrophils Are Characterized By Overexpression of P-Selectin Glycoprotein Ligand 1, a Potential Therapeutic Target
Jason S Knight¹, Patrick S. Coit¹, He Meng¹, Srilakshmi Yalavarthi¹, Paul Renauer¹, Robert C Grenn¹, Levi F Mazza¹, Hui Wang², Daniel T Eitzman² and Amr H Sawalha¹, ¹Division of Rheumatology, University of Michigan, Ann Arbor, MI, ²Division of Cardiovascular Medicine, University of Michigan, Ann Arbor, MI
Background/Purpose: Treatment of the thrombotic manifestations of antiphospholipid syndrome (APS) primarily focuses on inhibiting clotting pathways. In an effort to identify upstream inflammatory targets that…
Abstract Number: 2058 • 2016 ACR/ARHP Annual Meeting
Ectonucleotidase-Mediated Protection of Lupus Mice from Exaggerated Immune Responses and Arterial Vasculopathy
Jason S Knight¹, Levi F Mazza¹, Srilakshmi Yalavarthi¹, Yogen Kanthi² and David J Pinsky², ¹Division of Rheumatology, University of Michigan, Ann Arbor, MI, ²Division of Cardiovascular Medicine, University of Michigan, Ann Arbor, MI
Background/Purpose: CD39 and CD73 are so-called ectonucleotidases, surface enzymes expressed by leukocytes and endothelial cells that jut into the extracellular space. There, they mediate the…
Abstract Number: 3017 • 2016 ACR/ARHP Annual Meeting
Elevated Neutrophil Extracellular Trap Levels Correlate with Anti-CCP3-IgG and Anti-CCP3-IgA Levels in the Sputum of Individuals at-Risk for Future Rheumatoid Arthritis
M. Kristen Demoruelle¹, Monica Purmalek², Heather Rothfuss³, Michael Weisman⁴, Lindsay Kelmenson¹, Michael Mahler⁵, Jill M. Norris⁶, Brian Cherrington³, Mariana Kaplan², V. Michael Holers¹ and Kevin D. Deane¹, ¹Rheumatology Division, University of Colorado Denver, Aurora, CO, ²Systemic Autoimmunity Branch, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD, ³Zoology and Physiology, University of Wyoming, Laramie, WY, ⁴Rheumatology, Cedars-Sinai Medical Center, Los Angeles, CA, ⁵Research and Development, Inova Diagnostics, San Diego, CA, ⁶Epidemiology, Colorado School of Public Health, Aurora, CO
Background/Purpose: The initial site of anti-citrullinated protein antibody (ACPA) generation in RA has been proposed to be a mucosal site. We have previously demonstrated ACPA…
Abstract Number: 1787 • 2015 ACR/ARHP Annual Meeting
Innate Immunity, Arterial Inflammation and Vascular Stiffness in Patients with Systemic Lupus Erythematosus
Monica Purmalek¹, Simantini Sakhardande¹, Yenealem Temesgen-Oyelakin², Alice Fike³, Taufiq Salahuddin⁴, Balaji Natarajan⁴, Zerai Manna², Elizabeth Joyal², Sarfaraz Hasni², Nehal N. Mehta⁴ and Mariana J. Kaplan¹, ¹Systemic Autoimmunity Branch, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD, ²National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD, ³Office of the Clinical Director, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD, ⁴NHLBI, National Institutes of Health, Bethesda, MD
Background/Purpose: Patients with systemic lupus erythematosus (SLE) show a striking increase in risk of atherosclerotic cardiovascular disease (CVD) not explained by Framingham risk, compared to…
Abstract Number: 2002 • 2015 ACR/ARHP Annual Meeting
Dnase Treatment Protects Against DVT Formation in a Mouse Model of Antiphospholipid Syndrome
He Meng¹, Srilakshmi Yalavarthi², Levi F. Mazza³, Megan A. Elfline¹, Catherine E. Luke¹, Peter K. Henke¹ and Jason S. Knight², ¹University of Michigan, Ann Arbor, MI, ²Division of Rheumatology, University of Michigan, Ann Arbor, MI, ³Department of Internal Medicine, Division of Rheumatology, University of Michigan, Ann Arbor, MI
Background/Purpose: Antiphospholipid antibodies, especially those targeting beta-2-glycoprotein I (β2GPI), have been shown to amplify thrombosis in mice. However, most published models have relied on endothelial…
Abstract Number: 2072 • 2015 ACR/ARHP Annual Meeting
Mitochondrial Reactive Oxygen Species Modulate Autoimmunity in Systemic Lupus Erythematosus
Luz P. Blanco¹, Christian Lood², Monica Purmalek³, Suk See DeRavin⁴, Carolyne K. Smith³, Carmelo Carmona-Rivera³, Harry Malech⁴, Jeffrey Ledbetter⁵, Keith B. Elkon⁶ and Mariana J. Kaplan¹, ¹Systemic Autoimmunity Branch, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD, ²Internal Medicine, University of Washington, Seattle, WA, ³Systemic Autoimmunity Branch, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD, ⁴NIAID/NIH, Bethesda, MD, ⁵University of Washington, Seattle, WA, ⁶Department of Medicine & Immunology, University of Washington, Seattle, WA
Background/Purpose: Dysregulation in the formation of neutrophil extracellular traps (NETs) may contribute to modification and externalization of autoantigens and to organ damage in diverse autoimmune…
Abstract Number: 2073 • 2015 ACR/ARHP Annual Meeting
Mitochondrial ROS Is a Novel Regulator of Sting-Mediated Type I IFN Production By Governing Extrusion of Oxidized Mitochondrial DNA upon Neutrophil Extracellular Trap Formation.
Christian Lood¹, Luz P. Blanco², Monica Purmalek³, Carolyne K. Smith³, Carmelo Carmona-Rivera³, Jeffrey Ledbetter⁴, Mariana J. Kaplan² and Keith B. Elkon⁵, ¹Department of Medicine, Division of Rheumatology, University of Washington, Seattle, WA, ²Systemic Autoimmunity Branch, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD, ³Systemic Autoimmunity Branch, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD, ⁴University of Washington, Seattle, WA, ⁵Division of Rheumatology, University of Washington, Seattle, WA
Background/Purpose: Neutrophil extracellular trap generation (NETosis) is a reactive oxygen species (ROS)-dependent cell death pathway implicated in autoimmune disorders such as systemic lupus erythematosus (SLE).…
Abstract Number: 2121 • 2015 ACR/ARHP Annual Meeting
Placentas of Lupus Pregnancies Are Characterized By Marked Inflammatory Changes Despite Good Disease Control
Wendy Marder¹, Jason S. Knight², Mariana J. Kaplan³, Emily C. Somers⁴, Xu Zhang⁴, Alexander A. O'Dell⁵, Vasantha Padmanabhan⁴ and Richard W. Lieberman⁴, ¹Div of Rheumatology, University of Michigan, Ann Arbor, MI, ²Division of Rheumatology, University of Michigan, Ann Arbor, MI, ³National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD, ⁴University of Michigan, Ann Arbor, MI, ⁵Department of Internal Medicine, Division of Rheumatology, University of Michigan, Ann Arbor, MI
Background/Purpose: Systemic lupus erythematosus (SLE) is associated with increased risk of adverse pregnancy outcomes (APO), including preeclampsia and intrauterine growth restriction. While significant placental…
Abstract Number: 2598 • 2015 ACR/ARHP Annual Meeting
Neutrophil Extracellular Trap Levels Measured By Cell Free Nucleosome ELISA in Serum Are Highly Specific and Sensitive for Rheumatoid Arthritis
Paul Hasler¹, Stavros Giaglis¹, Ulrich A. Walker², Erik Deman¹, Christoph Hemmeler¹, Christina Ryser¹, Samuel Kretzschmar¹, Guenther Schaefer³, Juliane Franz¹ and Sinuhe Hahn⁴, ¹Rheumatology, Kantonsspital Aarau, Aarau, Switzerland, ²Rheumatology, University Hospital, Basel, Basel, Switzerland, ³Dept. of Biomedicine, Laboratory of Prenatal Medicine, University Hospital Basel, Basel, Switzerland, ⁴Dept. of Biomedicine, Laboratory for Prenatal Medicine, University Hospital Basel, Basel, Switzerland
Background/Purpose: Rheumatoid factor (RF) or anti-citrullinated peptide antibody (ACPA) detection have limited clinical utility for the diagnosis of rheumatoid arthritis (RA), as the specificity and…
Abstract Number: 2709 • 2015 ACR/ARHP Annual Meeting
Immediate Release of Peripheral Neutrophil Myeloperoxidase and Elastase and Formation of Extracellular Traps to Cigarette Smoking in Rheumatoid Arthritis
Juliane Franz¹, Stavros Giaglis^1,2, Guenther Schaefer³, Erik Deman¹, Andreas Thueler⁴, Sinuhe Hahn² and Paul Hasler¹, ¹Rheumatology, Kantonsspital Aarau, Aarau, Switzerland, ²Biomedicine, Laboratory of Prenatal Medicine, University Hospital Basel, Basel, Switzerland, ³Dept. of Biomedicine, Laboratory of Prenatal Medicine, University Hospital Basel, Basel, Switzerland, ⁴Rheumatology, Kantonsspital Baden, Baden, Switzerland
Background/Purpose: Smoking represents an independent risk factor for rheumatoid arthritis (RA) (1). In response to infectious agents, neutrophil granulocytes can extrude their nuclear contents as…
Abstract Number: 3032 • 2015 ACR/ARHP Annual Meeting
Role of Adenosine and Neutrophils in Inflammation Associated with Mutations in CECR1 Gene
Kyawt W. Shwin^1,2, Carmelo Carmona-Rivera³, Wanxia Tsai⁴, Chya-chia Richard Lee^5,6, Elaine Novakovich⁷, Deborah L. Stone⁸, Amanda K. Ombrello⁸, Raphaela Goldbach-Mansky⁹, Massimo Gadina¹⁰, Daniel Kastner¹¹, Ivona Aksentijevich^12,13, Mariana J. Kaplan⁷ and Peter C. Grayson^14,15, ¹NIAMS, National Institutes of Health, BETHESDA, MD, ²Translational Autoinflammatory Diseases Section, National Institutes of Health, Bethesda, MD, ³Systemic Autoimmunity Branch/ NIAMS, National Institutes of Health, Bethesda, MD, ⁴Translational Immunology, Office of Science and Technology, National Institutes of Health, Bethesda, MD, ⁵National Cancer Institute, National Institutes of Health, Bethesda, MD, ⁶Laboratory of Pathology, NCI, National Institutes of Health, BETHESDA, MD, ⁷Systemic Autoimmunity Branch, NIAMS, National Institutes of Health, Bethesda, MD, ⁸National Human Genome Research Institute, National Institutes of Health, Bethesda, MD, ⁹Translational Autoinflammatory Diseases Section, NIAMS, NIH, Bethesda, MD, ¹⁰Translational Immunology, Office of Science and Technology, NIAMS, National Institutes of Health, Bethesda, MD, ¹¹Inflammatory Disease Section, NHGRI, National Institutes of Health, Bethesda, MD, ¹²NIH/NHGRI, Metabolic, Cardiovascular, and Inflammatory Disease Genomics Branch, Bethesda, MD, ¹³Inflammatory Disease Section, National Human Genome Research Institute, Bethesda, MD, ¹⁴National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD, ¹⁵NIAMS Systemic Autoimmunity Branch, National Institutes of Health, Bethesda, MD
Background/Purpose: The phenotype of patients afflicted by deficiency of adenosine deaminase 2 (DADA2) due to mutations in CECR1encoding ADA2 protein shares many features with idiopathic…
Abstract Number: 205 • 2015 ACR/ARHP Annual Meeting
Mode of Action of Therapeutic Anti-Citrullinated Protein Antibodies Revealed: Inflammation Reduction Due to Inhibition of Netosis
Renato G.S. Chirivi^1,2, Jos W.G. van Rosmalen¹, Gonny Schmets¹, Helmuth van Es² and Jos M.H. Raats^1,2, ¹ModiQuest BV, Oss, Netherlands, ²Citryll BV, Nijmegen, Netherlands
Background/Purpose: Neutrophils together with aberrant Neutrophil Extracellular Trap (NET) formation contribute to the induction and propagation of inflammation. A growing number of studies indicate that…

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