Abstracts tagged "citrullination"

Abstract Number: 1070 • 2018 ACR/ARHP Annual Meeting
Seroreactivity Against Recombinant Citrullinated Myosin Is Associated with Measures of Diastolic Dysfunction in Patients with Rheumatoid Arthritis
Christian Geier¹, Justyna Fert-Bober², Jon T. Giles¹, Cesare Russo¹, Sabahat Bokhari³, Aylin Tugcu¹, Jenny Van Eyk² and Joan Bathon¹, ¹Columbia University, New York, NY, ²Cedars-Sinai Medical Center, Los Angeles, CA, ³Cardiology, Columbia University, New York, NY
Background/Purpose: Diastolic dysfunction and heart failure with preserved EF are more prevalent in RA. We have previously shown increased staining for citrullinated substrates in necropsied…
Abstract Number: 2049 • 2018 ACR/ARHP Annual Meeting
Expression and Function of a Novel Citrullinated Form of Interleukin 6 in Rheumatoid Arthritis
Chenyang Lu, Ray A. Ohara, Phillip L. Campbell, Yuxuan Du, W. Alexander Stinson, Jonatan Hervoso, Ellen Cealey, David Fox and M. Asif Amin, Division of Rheumatology and Clinical Autoimmunity Center of Excellence, Department of Internal Medicine, University of Michigan, Ann Arbor, MI, Ann Arbor, MI
Background/Purpose: Rheumatoid arthritis (RA) is a chronic inflammatory disorder characterized by synovial hyperplasia, progressive joint destruction and the presence of anti-citrullinated peptide antibodies (ACPAs) in…
Abstract Number: 485 • 2017 ACR/ARHP Annual Meeting
Different Citrullination Profiles in Spontaneous Versus Leukemia-Associated Rheumatoid Arthritis
Tal Gazitt¹, Son Hong Nguyen², Ari Salinger², Christian Lood³, Xizhang Sun¹, Lena M. Tanaka¹, David Feith⁴, Jeffrey Ledbetter⁵, Gordon Starkebaum⁶, Thomas Loughran Jr.⁷, Paul R. Thompson² and Keith B. Elkon⁸, ¹Division of Rheumatology, University of Washington, Seattle, WA, ²Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA, ³Division of Rheumatology, Division of Rheumatology, Department of Medicine, University of Washington, Seattle, WA, ⁴Hematology and Oncology, University of Virginia, Charlottesville, VA, ⁵University of Washington, Seattle, WA, ⁶Department of Medicine, Division of Rheumatology, University of Washington, Seattle, WA, ⁷Hematology Oncology, University of Virginia, Charlottesville, VA, ⁸Division of Rheumatology, Department of Medicine, University of Washington, Seattle, WA
Background/Purpose: Protein citrullination, the post-translational conversion of arginine to citrulline, mediated by peptidylarginine deiminase (PAD) enzymes, is considered a likely mechanism for the stimulation of…
Abstract Number: 837 • 2017 ACR/ARHP Annual Meeting
Rheumatoid Arthritis and Risk for Chronic Obstructive Pulmonary Disease or Asthma Among Women during 38 Years of Prospective Follow-up
Jeffrey A. Sparks¹, Tzu-Chieh Lin², Carlos Camargo³, Medha Barbhaiya⁴, Sara K. Tedeschi⁵, Karen H. Costenbader², Benjamin Raby⁶, Hyon K. Choi⁷ and Elizabeth Karlson⁴, ¹Rheumatology, Immunology, and Allergy, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, ²Division of Rheumatology, Immunology and Allergy, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, ³Emergency Medicine, Massachusetts General Hospital and Harvard Medical School, Boston, MA, ⁴Rheumatology, Immunology and Allergy, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, ⁵Division of Rheumatology, Immunology and Allergy,, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, ⁶Pulmonary Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, ⁷Division of Rheumatology, Allergy, and Immunology, Massachusetts General Hospital, Harvard Medical School, Boston, MA
Background/Purpose : Rheumatoid arthritis (RA) has been associated with increased risk for chronic obstructive pulmonary disease (COPD) and asthma, but these findings may have been…
Abstract Number: 470 • 2016 ACR/ARHP Annual Meeting
Crucial Involvement of Citrullinated Proteins and ACPA in the Development of Peptide-Glucose-6-Phosphate Isomerase Induced Arthritis
Hoshimi Kawaguchi¹, Isao Matsumoto¹, Hiroshi Ebe¹, Naoto Umeda¹, Yuya Kondo¹, Hiroto Tsuboi² and Takayuki Sumida¹, ¹Department of Internal Medicine, Faculty of Medicine, University of Tsukuba, Tsukuba, Japan, ²Division of Clinical Immunology, Doctoral Programs in Clinical Science, Graduated School of Comprehensive Human Science, University of Tsukuba, Tsukuba, Japan
Background/Purpose: Various citrullinated proteins and anti-citrullinated protein antibodies (ACPA) are increased in patients with rheumatoid arthritis (RA). ACPA have high specificity for RA, and are…
Abstract Number: 581 • 2016 ACR/ARHP Annual Meeting
Cell-Mediated Neutrophil Lysis-a Mechanism Promoting Hypercitrullination in Rheumatoid Arthritis?
Tal Gazitt¹, Christian Lood¹, Xizhang Sun², David Feith³, Jeffrey Ledbetter², Gordon Starkebaum¹, Thomas Loughran Jr.⁴ and Keith B. Elkon¹, ¹Department of Medicine, Division of Rheumatology, University of Washington, Seattle, WA, ²University of Washington, Seattle, WA, ³Hematology and Oncology, University of Virginia, Charlottesville, VA, ⁴Hematology Oncology, University of Virginia, Charlottesville, VA
BACKGROUND/PURPOSE:Protein citrullination, the post-translational conversion of arginine to citrulline, mediated by peptidylarginine deiminase (PAD) enzymes, is considered a likely mechanism for the stimulation of anti-citrullinated…
Abstract Number: 913 • 2016 ACR/ARHP Annual Meeting
Aggregatibacter Actinomycetemcomitans-Induced Hypercitrullination Links Periodontal Infection to Autoimmunity in Rheumatoid Arthritis
Maximilian F. Konig^1,2, Loreto Abusleme³, Jesper Reinholdt⁴, Robert J. Palmer³, Kevon Sampson¹, Ricardo P. Teles⁵, Peter A. Nigrovic⁶, Antony Rosen¹, Jeremy Sokolove⁷, Jon T. Giles⁸, Niki M. Moutsopoulos³ and Felipe Andrade¹, ¹Division of Rheumatology, The Johns Hopkins University School of Medicine, Baltimore, MD, ²Department of Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, MA, ³National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD, ⁴Department of Biomedicine, Aarhus University, Aarhus, Denmark, ⁵Department of Periodontology, School of Dentistry, University of North Carolina at Chapel Hill, Chapel Hill, NC, ⁶Division of Rheumatology, Immunology and Allergy, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, ⁷Division of Immunology and Rheumatology, Stanford University School of Medicine, Stanford, CA, ⁸Columbia University, College of Physicians and Surgeons, Division of Rheumatology, New York, NY
Background/Purpose: A bacterial etiology of rheumatoid arthritis (RA) has been suspected since the beginnings of modern germ theory. Recent studies implicate mucosal surfaces as sites…
Abstract Number: 1461 • 2016 ACR/ARHP Annual Meeting
Modification of Proteins with Malondialdehyde-Acetaldehyde and Citrulline Elicit Antibody Responses in DBA/1J Mice
Peter M. Maloley¹, Michael J. Duryee², Carlos D. Hunter², James R. O'Dell³, Daniel R. Anderson¹, Ted R Mikuls⁴, Geoffrey M. Thiele¹ and Lynell W. Klassen³, ¹University of Nebraska Medical Center, Omaha, NE, ²Internal Medicine Division of Rheumatology, University of Nebraska Medical Center, Omaha, NE, ³Department of Internal Medicine, University of Nebraska Medical Center, Omaha, NE, ⁴Internal Medicine, Division of Rheumatology, University of Nebraska Medical Center, Omaha, NE
Background/Purpose: Previous studies have shown that proteins modified with malondialdehyde-acetaldehyde (MAA) and/or citrulline (CIT) co-localize in rheumatoid arthritis (RA) synovial tissues, are pro-inflammatory, and promote…
Abstract Number: 3020 • 2016 ACR/ARHP Annual Meeting
Citrullination of Inhibitor of DNA Binding-1 at Specific Locations Leads to Autoantigenicity in Rheumatoid Arthritis
Ray A. Ohara¹, Henriette A. Remmer², Phillip L. Campbell³, David A. Fox³ and Jeffrey H. Ruth³, ¹Division of Rheumatology, University of Michigan Medical Center, Ann Arbor, MI, ²Department of Biological Chemistry, University of Michigan Medical School, Ann Arbor, MI, ³Internal Medicine, Division of Rheumatology, University of Michigan Medical School, Ann Arbor, MI
Background/Purpose: Inhibitor of DNA binding-1 (Id1) is a nuclear transcription factor that regulates cell growth and differentiation via selective binding and sequestering of other transcription…
Abstract Number: 3148 • 2016 ACR/ARHP Annual Meeting
Peptidylarginine Deiminase 2 Is Required for Tumor Necrosis Factor Alpha Induced Citrullination and Arthritis, but Not Neutrophil Extracellular Trap Formation
Mandar Bawadekar¹, Daeun Shim¹, Ryan Rebernick¹, Chloe Peyton¹, Chad J. Johnson², Thomas F. Warner³, Dres Damgaard⁴, Claus Henrik Nielsen⁵, Anthony P. Nicholas⁶, Ger JM Pruijn⁷, Jeniel E. Nett⁸ and Miriam A. Shelef^1,9, ¹Department of Medicine, Division of Rheumatology, University of Wisconsin-Madison, Madison, WI, ²Department of Medicine, University of Wisconsin-Madison, Madison, WI, ³Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, WI, ⁴Institute for Inflammation Research, Center for Rheumatology and Spine Diseases, Copenhagen University Hospital, Rigshospitalet, Copenhagen, Denmark, ⁵Danish Rheumatologic Biobank and DANBIO registry, Rigshospitalet, Glostrup, Gentofte and Herlev University Hospital, Copenhagen, Denmark, ⁶Neurology, University of Alabama at Birmingham and Birmingham VA Medical Center, Birmingham, AL, ⁷Biomolecular Chemistry, Institute for Molecules and Materials and Radboud Institute for Molecular Life Sciences, Radboud University, Nijmegen, Netherlands, ⁸Medicine and Medical Microbiology and Immunology, University of Wisconsin-Madison, Madison, WI, ⁹William S. Middleton Memorial Veterans Hospital, Madison, WI
Background/Purpose: The presence of anti-citrullinated protein antibodies in rheumatoid arthritis points to a potential role for citrullination in disease pathogenesis. Peptidylarginine deiminases (PADs) catalyze the…
Abstract Number: 905 • 2015 ACR/ARHP Annual Meeting
Citrullinated Epithelial-Derived Neutrophil-Activating Peptide 78 (ENA-78/CXCL5) Induces Monocyte Migration Via JNK and NFκB Signaling Pathways
Ray A. Ohara¹, Nicholas Lepore², W. Alexander Stinson³, Gautam Edhayan³, Phillip L. Campbell¹, Sarah Arwani¹, Rachel Morgan¹, Jeffrey H. Ruth¹, Alisa E. Koch^1,4, David A. Fox¹ and M. Asif Amin¹, ¹Internal Medicine, Division of Rheumatology, University of Michigan Medical Center, Ann Arbor, MI, ²University of Michigan, Division of Rheumatology, University of Michigan Medical Center, Ann Arbor, MI, ³Division of Rheumatology, University of Michigan Medical Center, Ann Arbor, MI, ⁴Eli Lilly and Company, Indianapolis, IN
Background/Purpose: Rheumatoid arthritis (RA) is a chronic autoimmune disorder characterized by monocyte (MN) infiltration into the synovium. Citrullination is a post-translational modification of arginine to…
Abstract Number: 530 • 2015 ACR/ARHP Annual Meeting
Immunomodulation of Naïve RA Patients PBMCs with a Multi-Epitope Peptide Derived from Citrullinated Autoantigens
Smadar Gertel¹, Yehuda Shoenfeld² and Howard Amital³, ¹Sheba Medical Center, Zabludowicz Center for Autoimmune Diseases, affiliated to Sackler Faculty of Medicine, Tel-Aviv University, Ramat Gan, Israel, ²Head Dept of Medicine B, Sheba Medical Center, Ramat-Gan, Israel, ³Sheba Medical Center, Tel-Hashomer, Israel
Background/Purpose: Citrullinated peptides are major targets of disease-specific autoantibodies in rheumatoid arthritis (RA). We generated a multi-epitope citrullinated peptide (Cit-ME), derived from major prevalent citrullinated…
Abstract Number: 528 • 2015 ACR/ARHP Annual Meeting
RA33 Challenges the Paradigm of Autoantigen Selection in Rheumatoid Arthritis
Maximilian F. Konig¹, Jon Giles², Peter A. Nigrovic³ and Felipe Andrade¹, ¹Division of Rheumatology, The Johns Hopkins University School of Medicine, Baltimore, MD, ²Rheumatology, Columbia University Medical Center, NY, NY, ³Department of Medicine, Division of Rheumatology, Immunology and Allergy, Brigham and Women’s Hospital, Harvard Medical School, Boston, MA
Background/Purpose: Antibodies against citrullinated proteins (ACPAs) are the autoimmune hallmark of rheumatoid arthritis (RA), and can precede the onset of symptomatic disease by years. By…
Abstract Number: 521 • 2015 ACR/ARHP Annual Meeting
Targeted Quantitative Analysis to Identify Citrullinated Peptides in the Synovial Fluid of Patients with Rheumatoid Arthritis
Min Jueng Kang¹, Ji Soo Kim², Eugene C. Yi¹ and Eun Young Lee², ¹Molecular Medicine and Biopharmaceutical Sciences, Department of Molecular Medicine and Biopharmaceutical Sciences, Graduate School of Convergence Science and Technology, and College of Medicine or College of Pharmacy, Seoul National University, Seoul, South Korea, ²Division of Rheumatology, Department of Internal Medicine, Seoul National University College of Medicine, Seoul, South Korea
Background/Purpose: The protein/peptide citrullination is a post-translational modification and the citrullinated peptide epitopes are recognized by anti-citrullinated protein/peptide antibody (ACPA), which is a major diagnostic…
Abstract Number: 1946 • 2015 ACR/ARHP Annual Meeting
A Novel HLA-DRB1*10:01 Restricted T Cell Epitope from Citrullinated Type II Collagen Relevant for Rheumatoid Arthritis
Karine Chemin¹, Sabrina Pollastro², Eddie James³, Changrong Ge⁴, Inka Albrecht⁵, Jessica Herrath⁶, Christina Gerstner⁷, Thais Rizzi², Karolina Tandre⁸, Lars Rönnblom⁸, Anca I Catrina⁹, Rikard Holmdahl¹⁰, L. Klareskog¹¹, Niek De Vries¹² and Vivianne Malmström¹¹, ¹Medicine, Rheumatology Unit, Department of Medicine, Solna, Karolinska Institutet, Stockholm, Sweden, ²Department of Clinical Immunology and Rheumatology, Amsterdam, Netherlands, ³Benaroya Research Institute at Virginia Mason, Seattle, WA, ⁴Department of Medical Biochemistry and Biophysics, Medical Inflammation Research, Karolinska Institute, Stockholm, Sweden, ⁵Rheumatology Unit, Department of Medicine, Solna, Karolinska Institutet, Stockholm, Sweden, ⁶Department of Medicine,, Rheumatology Unit, Department of Medicine, Solna, Karolinska Institutet, Stockholm, Sweden, ⁷Department of Medicine, Rheumatology Unit, Karolinska University Hospital, Solna, Stockholm, Sweden, ⁸Section of Rheumatology, Department of Medical Sciences, Uppsala University, Uppsala, Sweden, ⁹Unit of Rheumatology, Department of Medicine Solna, Karolinska University Hospital, Karolinska Institutet, Stockholm, Sweden, ¹⁰Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden, ¹¹Rheumatology Unit, Department of Medicine, Karolinska Institutet, Stockholm, Sweden, ¹²Dept. of Clinical Immunology & Rheumatology, Amsterdam Rheumatology and immunology Center, Academic Medical Center/University of Amsterdam, Amsterdam, Netherlands
Background/Purpose: Antibodies against citrullinated collagen type II (Cit-CII) are common in sera and synovial fluids of Rheumatoid Arthritis (RA) patients, whereas the known CII T-cell…