ACR Meeting Abstracts

ACR Meeting Abstracts

  • Meetings
    • ACR Convergence 2024
    • ACR Convergence 2023
    • 2023 ACR/ARP PRSYM
    • ACR Convergence 2022
    • ACR Convergence 2021
    • ACR Convergence 2020
    • 2020 ACR/ARP PRSYM
    • 2019 ACR/ARP Annual Meeting
    • 2018-2009 Meetings
    • Download Abstracts
  • Keyword Index
  • Advanced Search
  • Your Favorites
    • Favorites
    • Login
    • View and print all favorites
    • Clear all your favorites
  • ACR Meetings

Abstract Number: 1472

Synovial Fluid from Rheumatoid Arthritis Patients Modulates the Immunophenotype and Viability of Monocytes

Maria Sole Chimenti, Alberto Bergamini, Eleonora Baffari, Eleonora Ballanti, Alessia Musto, Paola Conigliaro and Roberto Perricone, Rheumatology, allergology and clinical immunology, Department of "Medicina dei Sistemi", University of Rome "Tor Vergata", Rome, Italy

Meeting: 2014 ACR/ARHP Annual Meeting

Keywords: anti-TNF therapy, Auto-immunity, innate immunity, monocytes and rheumatoid arthritis (RA)

  • Tweet
  • Email
  • Print
Session Information

Title: Rheumatoid Arthritis - Human Etiology and Pathogenesis

Session Type: Abstract Submissions (ACR)

Background/Purpose

Rheumatoid arthritis (RA) is a chronic systemic autoimmune disease characterized by infiltration of the synovium by inflammatory cells that destroys the cartilage and the bone adjacent to the joint. The thickened synovium, called pannus, causes swelling of the joint with excess of synovial fluid (SF) production. A large number of proteins involved in inflammation and immune response have been identified in SF from RA patients (SF-RA). The aim of this study was to determine the effects of synovial fluids (SF) from RA patients (SF-RA) on surface phenotype, co-stimulatory activity, viability and cytokine production of monocytes, as a model for the interaction between SF and synovial tissue macrophages in RA. 

Methods

Purified monocytes from healthy donors were incubated with individual specimens of SF-RA obtained from twelve RA patients with active knee arthritis and then analyzed by flow cytometry for ILT4 and CD86 expression, co-stimulation ability, rate of spontaneous apoptosis and TNFα, IL-1β and IL-10 production. Comparative analysis was carried out with serum from RA patients and medium alone. 

Results

Downmodulation of ILT4 and upmodulation of CD86 expression (Figure 1), together with increased ability to co-stimulate CD4+ T cells for IFNγ and TNFα production, was observed in monocytes incubated with SF-RA (SF-RA monocytes) compared with control cells, even under condition of activation by CD40L (Figure 2). A reduction of spontaneous apoptosis was observed in SF-RA monocytes compared to control cells. Adalimumab increased the rate of SF-RA monocytes apoptosis, whereas no significant influence of adalimumab was detected in control monocytes. The TNFα and IL1β response, but not that of IL-10, to LPS was greater in SF-RA monocytes compared with control cells (Figure 3). 

Conclusion

Our findings suggest that soluble factors present in SF-RA could function as so-called damage-associated molecular patterns and contribute to increase the effectiveness intra-articular of the immune response and inflammation by increasing monocyte numbers and pro-inflammatory activity.


Disclosure:

M. S. Chimenti,
None;

A. Bergamini,
None;

E. Baffari,
None;

E. Ballanti,
None;

A. Musto,
None;

P. Conigliaro,
None;

R. Perricone,
None.

  • Tweet
  • Email
  • Print

« Back to 2014 ACR/ARHP Annual Meeting

ACR Meeting Abstracts - https://acrabstracts.org/abstract/synovial-fluid-from-rheumatoid-arthritis-patients-modulates-the-immunophenotype-and-viability-of-monocytes/

Advanced Search

Your Favorites

You can save and print a list of your favorite abstracts during your browser session by clicking the “Favorite” button at the bottom of any abstract. View your favorites »

All abstracts accepted to ACR Convergence are under media embargo once the ACR has notified presenters of their abstract’s acceptance. They may be presented at other meetings or published as manuscripts after this time but should not be discussed in non-scholarly venues or outlets. The following embargo policies are strictly enforced by the ACR.

Accepted abstracts are made available to the public online in advance of the meeting and are published in a special online supplement of our scientific journal, Arthritis & Rheumatology. Information contained in those abstracts may not be released until the abstracts appear online. In an exception to the media embargo, academic institutions, private organizations, and companies with products whose value may be influenced by information contained in an abstract may issue a press release to coincide with the availability of an ACR abstract on the ACR website. However, the ACR continues to require that information that goes beyond that contained in the abstract (e.g., discussion of the abstract done as part of editorial news coverage) is under media embargo until 10:00 AM ET on November 14, 2024. Journalists with access to embargoed information cannot release articles or editorial news coverage before this time. Editorial news coverage is considered original articles/videos developed by employed journalists to report facts, commentary, and subject matter expert quotes in a narrative form using a variety of sources (e.g., research, announcements, press releases, events, etc.).

Violation of this policy may result in the abstract being withdrawn from the meeting and other measures deemed appropriate. Authors are responsible for notifying colleagues, institutions, communications firms, and all other stakeholders related to the development or promotion of the abstract about this policy. If you have questions about the ACR abstract embargo policy, please contact ACR abstracts staff at [email protected].

Wiley

  • Online Journal
  • Privacy Policy
  • Permissions Policies
  • Cookie Preferences

© Copyright 2025 American College of Rheumatology