ACR Meeting Abstracts

ACR Meeting Abstracts

  • Home
  • Meetings Archive
    • ACR Convergence 2022
    • ACR Convergence 2021
    • ACR Convergence 2020
    • 2020 ACR/ARP PRSYM
    • 2019 ACR/ARP Annual Meeting
    • 2018 ACR/ARHP Annual Meeting
    • 2017-2009 Meetings
    • Download Abstracts
  • Keyword Index
  • Advanced Search
  • Your Favorites
    • Favorites
    • Login
    • View and print all favorites
    • Clear all your favorites
  • Meeting Resource Center

Abstract Number: 2799

Sjogren’s Syndrome Minor Salivary Gland CD4+ T Cells Associate with Oral Disease Features and Have a T Follicular Helper-like Transcriptional Profile

Michelle L. Joachims1, Kerry M. Leehan1, Mikhail Dozmorov1,2, Zijian Pan1, Astrid Rasmussen1, Lida Radfar3, David M. Lewis4, Donald U. Stone5,6, Kiely Grundahl7, R. Hal Scofield1,8,9, Christopher J. Lessard1, Jonathan Wren1, Linda F. Thompson1, Kathy L. Sivils10, Jacen Maier-Moore1,11 and A. Darise Farris1, 1Arthritis and Clinical Immunology Program, Oklahoma Medical Research Foundation, Oklahoma City, OK, 2Biostatistics, Virginia Commonwealth University, Richmond, VA, 3Department of Oral Diagnosis and Radiology, University of Oklahoma College of Dentistry, Oklahoma City, OK, 4Department of Oral and Maxillofacial Pathology, University of Oklahoma College of Dentistry, Oklahoma City, OK, 5Ophthalmology, Johns Hopkins University, Baltimore, MD, 6King Khaled Eye Specialist Hospital, Riyadh, Saudi Arabia, 7Arthritis and Clinical Immunology Research Program, Oklahoma Medical Research Foundation, Oklahoma CIty, OK, 8Department of Veterans Affairs Medical Center, Oklahoma City, OK, 9Medicine, University of Oklahoma Health Sciences Center, Oklahoma City, OK, 10Arthritis and Clinical Immunology Program, Oklahoma Medical Research Foundation, Oklahoma, OK, 11Clinical Laboratory Science, University of Texas at El Paso, El Paso, TX

Meeting: 2018 ACR/ARHP Annual Meeting

Keywords: CD T cells, Sjogren's syndrome and salivary gland

  • Tweet
  • Email
  • Print
Session Information

Date: Tuesday, October 23, 2018

Session Title: 5T092 ACR Abstract: T Cell Biology & Targets in Autoimmune & Inflammatory Disease (2797–2801)

Session Type: ACR Concurrent Abstract Session

Session Time: 2:30PM-4:00PM

Background/Purpose: The predominant salivary gland (SG) T cell types contributing to disease in Sjögren’s syndrome (SS) are unclear. This study assessed the frequency and number of SG CD3+ T cell subtypes for association with SS disease features and compared the SG CD4+ T cell transcriptome of primary SS subjects to that of sicca controls not meeting criteria for SS.

Methods: CD3+ T cells from SG biopsy tissue of subjects with primary SS and non-SS sicca were evaluated for proportion (n=51 SS, n=69 non-SS) and number/mg biopsy tissue (n=34 SS, n=56 non-SS) of T cell subsets defined by CD3, CD4, CD8 and CD45RA using flow cytometry. Proportions of memory CD4+ T cells were evaluated for correlation with clinical and oral disease parameters. Sorted salivary gland memory CD4+ T cells from a subset of focus score positive SS cases (n=17) and focus score negative non-SS subjects (n=15) were evaluated for global gene expression by microarray. Differentially expressed genes were assessed using the limma R package, and bioinformatics analyses were performed using Ingenuity Pathways Analysis and Gene Set Enrichment Analysis.

Results: Proportions of CD4+CD45RA– T cells (mean ± SEM pSS: 33.2%±2.0, non-SS: 21.9%±1.2, p<0.0001) but not those of other CD3+ T cell subsets were increased in SS cases compared to non-SS sicca subjects. Proportions of SG CD4+ memory T cells positively correlated with SG focus score (r=0.47, p<0.0001), morphologic area of SG fibrosis (r=0.35, p=0.006), and van Bijsterveld corneal damage score (r=0.37, p<0.0001), with relationships remaining after age correction. Differentially expressed (DE) genes in SS cases versus non-SS sicca subjects were enriched for T follicular helper (Tfh), interferon, T cell homeostasis, resistance to apoptosis, atypical lymphoid trafficking and elevated inflammatory response pathways, but not Th17 profile. Predicted upstream drivers of the DE genes included CXCL13, CD40/CD40 ligand and Bcl6, while predicted decreased effects included FoxP3, Fas, STAT6 and mTOR.

Conclusion: Proportion and number of SG memory CD4+ T cells selectively associate with key SS disease features, and SG memory CD4+ T cells are enriched for a predominant Tfh-like cell profile.


Disclosure: M. L. Joachims, None; K. M. Leehan, None; M. Dozmorov, None; Z. Pan, None; A. Rasmussen, None; L. Radfar, None; D. M. Lewis, None; D. U. Stone, None; K. Grundahl, None; R. H. Scofield, NIH, ept Veran Affirs,Lupus esearch Institute, 2,Dept of Veterans Affairs, Universiy of Oklahoma, 3,Boston Pharmacueticals, 5; C. J. Lessard, None; J. Wren, None; L. F. Thompson, None; K. L. Sivils, None; J. Maier-Moore, None; A. D. Farris, None.

To cite this abstract in AMA style:

Joachims ML, Leehan KM, Dozmorov M, Pan Z, Rasmussen A, Radfar L, Lewis DM, Stone DU, Grundahl K, Scofield RH, Lessard CJ, Wren J, Thompson LF, Sivils KL, Maier-Moore J, Farris AD. Sjogren’s Syndrome Minor Salivary Gland CD4+ T Cells Associate with Oral Disease Features and Have a T Follicular Helper-like Transcriptional Profile [abstract]. Arthritis Rheumatol. 2018; 70 (suppl 9). https://acrabstracts.org/abstract/sjogrens-syndrome-minor-salivary-gland-cd4-t-cells-associate-with-oral-disease-features-and-have-a-t-follicular-helper-like-transcriptional-profile/. Accessed February 6, 2023.
  • Tweet
  • Email
  • Print

« Back to 2018 ACR/ARHP Annual Meeting

ACR Meeting Abstracts - https://acrabstracts.org/abstract/sjogrens-syndrome-minor-salivary-gland-cd4-t-cells-associate-with-oral-disease-features-and-have-a-t-follicular-helper-like-transcriptional-profile/

Advanced Search

Your Favorites

You can save and print a list of your favorite abstracts during your browser session by clicking the “Favorite” button at the bottom of any abstract. View your favorites »

ACR Pediatric Rheumatology Symposium 2020

© COPYRIGHT 2023 AMERICAN COLLEGE OF RHEUMATOLOGY

Wiley

  • Home
  • Meetings Archive
  • Advanced Search
  • Meeting Resource Center
  • Online Journal
  • Privacy Policy
  • Permissions Policies
  • Cookie Preferences