Near Patient Detection of Anti-MDA5 Antibodies Using Photonic Ring Immunoassays

Makoto Miyara¹, Rémi Chieze², Yurdagul Uzunhan³, Jean- Luc Charuel⁴, Pascale Ghillani-Dalbin⁴, Sasi Mudumba⁵, Alice Wu⁵, Hilario Nunes³, Zahir Amoura⁶, Rufus Burlingame⁵ and Lucile Musset¹, ¹Department of immunology, Pitié-Salpêtrière Hospital (AP-HP), Paris, France, ²department of Immunology, Pitié-Salpêtrière Hospital (AP-HP), Paris, France, ³Pulmonary diseases department, Avicenne Hospital (AP-HP), Bobigny, France, ⁴Department of Immunology, Pitié-Salpêtrière Hospital (AP-HP), Paris, France, ⁵Genalyte Inc., San Diego, CA, ⁶Hôpital Pitié-Salpêtrière, AP-HP, UPMC Univ Paris 06 & French National Reference Center For Systemic Lupus and Antiphospholipid Syndrome, Paris, France

Meeting: 2017 ACR/ARHP Annual Meeting

Date of first publication: September 18, 2017

Keywords: autoantibodies, dermatomyositis, diagnosis and interstitial lung disease

Session Information

Date: Tuesday, November 7, 2017

Title: Muscle Biology, Myositis and Myopathies Poster

Session Type: ACR Poster Session C

Session Time: 9:00AM-11:00AM

Background/Purpose:

The presence of anti-MDA5 antibody is associated with amyopathic dermatomyositis and/or rapidly progressive interstitial lung disease that can be fatal.In the latter case, it is mandatory to confirm the diagnosis as soon as possible in order to initiate aggressive treatments and the presence of anti-MDA5 autoantibodies is an important part of the differential diagnosis. The immunoassay using photonic microrings is a new sensitive technology that enables rapid results in less than 15 minutes. The aim of our study was to evaluate this technology for the detection of anti-MDA5 antibodies in sera with known positivity.

Methods: The system is based on immobilizing the antigenic target, MDA5, above the photonic rings in a silicon chip. We analyzed a total of 72 sera drawn from 40 patients known to be positive for anti-MDA5 antibody in our laboratory using immunoblotting assays and 4 negative controls. For some patients, several samples drawn during the follow-up could be analyzed.

Results: Using the photonic ring immunoassay, we could confirm the presence of anti-MDA5 antibody in all samples known as positive for anti-MDA5 antibody by other techniques. No false positive or false negative results were found. Moreover, high levels of MDA-5 antibodies measured at diagnosis are associated withinterstitial lung disease with respiratory functional impairment at presentation. Interstingly, we observed a decrease in anti-MDA5 antibodies titers in followed-up patients.

Conclusion: The photonic ring immunoassay is a fast and reliable technology for the detection of anti-MDA5 antibodies.

Disclosure: M. Miyara, Genalyte, 5; R. Chieze, None; Y. Uzunhan, None; J. L. Charuel, None; P. Ghillani-Dalbin, None; S. Mudumba, None; A. Wu, Genalyte, 3; H. Nunes, None; Z. Amoura, None; R. Burlingame, None; L. Musset, None.

To cite this abstract in AMA style:

Miyara M, Chieze R, Uzunhan Y, Charuel JL, Ghillani-Dalbin P, Mudumba S, Wu A, Nunes H, Amoura Z, Burlingame R, Musset L. Near Patient Detection of Anti-MDA5 Antibodies Using Photonic Ring Immunoassays [abstract]. Arthritis Rheumatol. 2017; 69 (suppl 10). https://acrabstracts.org/abstract/near-patient-detection-of-anti-mda5-antibodies-using-photonic-ring-immunoassays/. Accessed .

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