Session Type: ACR Poster Session C
Session Time: 9:00AM-11:00AM
Background/Purpose: This study aimed to determine the regulatory role of N-Acetyl-L-cysteine (NAC), an antioxidant, in T cell and osteoclast differentiation in rheumatoid arthritis (RA).
Methods: After RA synovial fibroblasts were stimulated by LPS, the expression and production of RANKL, IL-1beta, TNF-alpha was determined by real-time PCR and ELISA. After human peripheral CD4+ T cells were cultured under Th0 condition, IL-17, IFN-g, IL-4, Foxp3, RANKL and IL-2 expression was determined by flow cytometry and ELISA. Human peripheral blood monocytes were cultured with M-CSF, RANKL, and various concentrations of NAC, followed by staining of the cells for tartrate-resistant acid phosphatase activity to determine osteoclast formation. Osteoclastogenesis was also determined after cocultures of LPS-stimulated RA synovial fibroblasts or Th0-stimulated CD4+T cells and various concentrations of NAC with human PBMC.
Results: When RA synovial fibroblasts were stimulated by LPS, LPS stimulated their production of RANKL, IL-1beta, TNF-alpha and IL-6. NAC reduced the LPS-induced production of proinflammatory cytokines and RANKL in a dose-dependent manner. After human peripheral CD4+ T cells were cultured under Th0 polarizing condition, NAC decreased the proportion of IL-17+ and CD4+ T cells, and production of IL-17 and RANKL. When human peripheral blood CD14+ monocytes were cultured with M-CSF and RANKL, osteoclasts was differentiated, however, NAC significantly inhibited the osteoclastogenesis
Conclusion: NAC inhibits the production of proinflammatory cytokines and RANKL in RA synovial fibroblasts, differentiation of osteoclasts and Th17 cells. NAC could be a new therapeutic medication for regulation of autoimmune reaction and prevention of bone destruction in RA.
To cite this abstract in AMA style:Lee KA, Kim HR, Lee SH, Kim B, Kim KW. N-Acetylcysteine Regulates Osteoclastogenesis and Th17 Cell Differentiation in Rheumatoid Arthritis [abstract]. Arthritis Rheumatol. 2016; 68 (suppl 10). https://acrabstracts.org/abstract/n-acetylcysteine-regulates-osteoclastogenesis-and-th17-cell-differentiation-in-rheumatoid-arthritis/. Accessed January 25, 2022.
« Back to 2016 ACR/ARHP Annual Meeting
ACR Meeting Abstracts - https://acrabstracts.org/abstract/n-acetylcysteine-regulates-osteoclastogenesis-and-th17-cell-differentiation-in-rheumatoid-arthritis/