ACR Meeting Abstracts

ACR Meeting Abstracts

Abstract Number: 1577

Interleukin-37: Associations of Plasma Levels and Genetic Variants in Gout

Adela Navratilova1, Veronika Voclonová2, Hana Hulejová2, Lucie Andrés Cerezo1, Markéta Pavlíková3, Viktor Bečvář2, Jakub Zavada1, Karel Pavelka4, Ladislav Senolt4 and Blanka Stiburkova5, 1Institute of Rheumatology and Department of Rheumatology, First Faculty of Medicine, Charles University, Prague, Czech Republic, 2Institute of Rheumatology, Prague, Czech Republic, 3Department of Probability and Mathematical Statistics, Faculty of Mathematics and Physics, Charles University, Prague, Czech Republic, 4Institute of Rheumatology, Department of Rheumatology, 1st Faculty of Medicine, Charles University, Prague, Czech Republic, 5Institute of Rheumatology and Department of Pediatrics and Inherited Metabolic Disorders, First Faculty of Medicine, Charles University and General University Hospital in Prague, Prague, Czech Republic, Prague, Czech Republic

Meeting: ACR Convergence 2021

Keywords: , ,

Session Information

Date: Tuesday, November 9, 2021

Title: Metabolic & Crystal Arthropathies – Basic & Clinical Science Poster II (1565–1583)

Session Type: Poster Session D

Session Time: 8:30AM-10:30AM

Background/Purpose: IL-37 is an anti-inflammatory cytokine, member of IL-1 family, related to inflammatory and autoimmune diseases. Here we aimed to investigate the association of genetic variants of IL-37 and IL-37 plasma levels in relation to hyperuricemia/gout.

Methods: The study involved normouricemic controls (n=50), primary hyperuricemia (n=100), primary gout (n=100), tophaceous gout (n=25) and acute gout flare (n=18). All patients met the ACR gout classification criteria (1). Levels of plasma IL-37 were analysed using Enzyme-Linked Immunosorbent Assay. All coding regions and intron-exon boundaries of IL-37, exon 1-5, were amplified and sequenced directly. Differences of measured levels of IL-37 between groups were compared using Wilcoxon test. Variant allele frequencies were compared using binomial test and Fisher exact test.

Results: IL-37 levels were fairly similar across groups except for patients with tophaceous gout that had IL-37 levels significantly increased in contrast to control subjects (p=0.019) and gout patients (p=0.036). We identified 12 IL-37 genetic variants: five intron (rs28947188, rs2466448, rs3811045, rs3811048, rs2708944), and seven non-synonymous allelic variants (rs3811046, rs3811047, rs2708943, rs2723183, rs2723187, rs2708947, rs27231927). Of those, rs28947188 showed a significant under-presentation in hyperuricemic and gout cohorts (no variant found among all 243 patients) compared to normouricemic control group (minor allele frequency: MAF = 0.05) and European population (MAF = 0.083).

Conclusion: Variant rs28947188 showed a significant under-presentation in hyperuricemic/gout cohorts compared to European population MAF/normouricemic cohort. Plasma IL-37 is significantly up-regulated in patients with tophaceous gout compared to other groups. This evidence highlights the role of IL-37 in the pathogenesis of gout.

References:

Neogi T, Jansen TL, Dalbeth N, et al. 2015 Gout classification criteria: an American College of Rheumatology/European League Against Rheumatism collaborative initiative. Ann Rheum Dis. 2015;74:1789-98.

Acknowledgement:

Supported by MHCR 023728, SVV 260 523 and BBMRI-CZ LM2018125

Disclosures: A. Navratilova, None; V. Voclonová, None; H. Hulejová, None; L. Andrés Cerezo, None; M. Pavlíková, None; V. Bečvář, None; J. Zavada, None; K. Pavelka, Abbvie, 6, UCB, 6, MSD, 6, Roche, 6, Pfizer, 6, Eli Lilly, 6, Egis, 6, Biogen, 6, Pfizer, 6; L. Senolt, None; B. Stiburkova, None.

To cite this abstract in AMA style:

Navratilova A, Voclonová V, Hulejová H, Andrés Cerezo L, Pavlíková M, Bečvář V, Zavada J, Pavelka K, Senolt L, Stiburkova B. Interleukin-37: Associations of Plasma Levels and Genetic Variants in Gout [abstract]. Arthritis Rheumatol. 2021; 73 (suppl 9). https://acrabstracts.org/abstract/interleukin-37-associations-of-plasma-levels-and-genetic-variants-in-gout/. Accessed .

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