ACR Meeting Abstracts

ACR Meeting Abstracts

Abstract Number: 272

Interferon Chemokine Score and Other Cytokine Measures Predict Changes in Disease Activity in Patients with Juvenile and Adult Dermatomyositis

Cynthia S. Crowson1, Jeannette M. Olazagasti Lourido2, Molly S. Hein3, Richard S. Pendegraft4, Michael A. Strausbauch5, Timothy B. Niewold6, Floranne C. Ernste7, Theresa L. Wampler Muskardin3, Erik J. Peterson8, Emily C. Gillespie9 and Ann M Reed10, 1Health Sciences Research, Mayo Clinic, Rochester, MN, 2University of Puerto Rico, San Juan, Puerto Rico, 3Rheumatology, Mayo Clinic, Rochester, MN, 4Biomedical Statistics and informatics, Rochester, MN, 5Surgical Research, Mayo Clinic, Rochester, MN, 6Rheumatology and Immunology, Mayo Clinic, Rochester, MN, 7Division of Rheumatology, Mayo Clinic Rochester, Rochester, MN, 8Center for Immunology/Department of Medicine, University of Minnesota, Minneapolis, MN, 9Medicine, University of Minnesota, Minneapolis, MN, 10Rheumatology, Duke University, Durham, NC

Meeting: 2016 ACR/ARHP Annual Meeting

Date of first publication: September 28, 2016

Keywords: , , ,

Session Information

Date: Sunday, November 13, 2016

Title: Muscle Biology, Myositis and Myopathies - Poster I: Basic/Translational

Session Type: ACR Poster Session A

Session Time: 9:00AM-11:00AM

Background/Purpose:   Serum cytokines play an important role in the pathogenesis of myositis by initiating and perpetuating various cellular and humoral autoimmune processes. The aim of this study was to measure interferon (IFN)-inducible chemokines (IFNCK), Th1, Th2, Th17, innate, and regulatory cytokines in patients with adult dermatomyositis (DM) and juvenile dermatomyositis (JDM) at multiple visits to identify biomarkers predictive of changes in disease activity.

Methods:   Multiplexed immunoassays (Meso Scale Discovery) enabled simultaneous measurement of IFN-regulated chemokines and other pro- and anti-inflammatory cytokines specific to differentiation of specific T cell and innate pathways. Cytokine scores were computed for IFNCK (IP-10, MCP-1), Th1 (IFNɣ, TNFα, and IL2), Th2 (IL4, IL10, IL12, and 1L 13), Th17 (IL6, IL17, IL1β), innate (MIP-1α, MIP-1β, IL8), and regulatory (IL10, TNFα) factors. Spearman correlation was used to examine whether cytokines at a previous visit predict change at the next visit, adjusting for disease activity at the previous visit.

Results:   The study included 30 patients (13 DM and 17 JDM) with at least 2 visits (68 visits total). Mean age (SD) at inclusion was 53.3 (18.6) years in DM and 9.9 (5.6) years in JDM, 70% female, 87% Caucasian. Most patients were included at their time of diagnosis.  The mean (SD) physician global, muscle and extra-muscular disease activity VAS scores at inclusion were 45 (24), 40(30) and 35(20) cm, respectively. The IFNCK score predicted change in physician global (r=0.30; p=0.012), muscle (r=0.29; p=0.016) and extra-muscular (r=0.24; p=0.05) disease activity. The Th17 score also predicted change in physician global (r=0.28; p=0.020), muscle (r=0.25; p=0.041) in DM and JDM, but extra-muscular only in DM (r=0.41; p=0.029). IL6 levels also predicted change in physician global (r=0.31; p=0.011), muscle (r=0.33; p=0.006) and extra-muscular (r=0.33; p=0.007) disease activity, but these associations were predominately among DM.

Conclusion:   IFNCK and other cytokine scores may be useful biomarkers to predict changes in disease activity among myositis patients. Differing associations for JDM and DM may indicate biological differences between diseases that warrant specific biomarkers for each to best predict changes in disease activity.

Disclosure: C. S. Crowson, None; J. M. Olazagasti Lourido, None; M. S. Hein, None; R. S. Pendegraft, None; M. A. Strausbauch, None; T. B. Niewold, None; F. C. Ernste, None; T. L. Wampler Muskardin, None; E. J. Peterson, None; E. C. Gillespie, None; A. M. Reed, None.

To cite this abstract in AMA style:

Crowson CS, Olazagasti Lourido JM, Hein MS, Pendegraft RS, Strausbauch MA, Niewold TB, Ernste FC, Wampler Muskardin TL, Peterson EJ, Gillespie EC, Reed AM. Interferon Chemokine Score and Other Cytokine Measures Predict Changes in Disease Activity in Patients with Juvenile and Adult Dermatomyositis [abstract]. Arthritis Rheumatol. 2016; 68 (suppl 10). https://acrabstracts.org/abstract/interferon-chemokine-score-and-other-cytokine-measures-predict-changes-in-disease-activity-in-patients-with-juvenile-and-adult-dermatomyositis/. Accessed .

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