Session Title: T cell Biology in Rheumatoid Arthritis and Other Arthritis
Session Type: Abstract Submissions (ACR)
Mesenchymal stem cells (MSC) have been isolated from synovium and represent a fraction of synovial fibroblast (SF) cultures. CD271+ is considered a MSC surface marker that is also present in a small fraction of primary cultures of SF. Bone marrow or synovial derived CD271+ cells have shown increased chondrogenic potential but their immunosuppressive properties have not been analyzed. We have analyzed the immunosuppressive potential of CD271+ compared to CD271- primary SF cultures from human osteoarthritic synovial tissues.
Osteoarthritic synovial membranes were obtained at knee joint replacement surgery (n=9). CD271+/- separation was carried out by magnetic sorting of passage 0 SF cultures and confirmed by flow cytometry. Multipotent differentiation capability of SF to adipocytes, chondroblasts and osteoclasts was studied using standard in vitrotissue culture-differentiating conditions and staining with oil red, alcian blue or alizarin red, respectively. Immunomodulatory properties of CD271+/- sorted cells were analyzed in co-cultures with T lymphocytes obtained from healthy donors (ratio 1:10), stimulated with anti-CD3/CD28 beads. T-cell proliferation was measured by CellVue dye dilution as detected by flow cytometry. T-cell cytokine production (IL-2, IL-10, IL-17 and IFN-γ) in supernatants was quantified by ELISA. Quantitative data were analyzed by Mann Whitney or one-sample t-test.
Primary passage 0 OA SF cultures contained (11.27±7.25%) CD271+ cells. After sorting, both CD271+ and CD271- SF cultures contained multipotential MSC capacity to differentiate to adipocytes, chondroblasts and osteoclasts in specific differentiation media. Both types of cells significantly inhibited the proliferation of anti-CD3/CD28 stimulated T cells and there were no quantitative differences between CD271+ and CD271- cells (70.46±6.56% and 73.53±13.15% respectively compared to single T-cell parallel cultures where proliferation was set to 100%). Co-culture of SF and T lymphocytes induced a significant increase in IL-2, IL-10, IL-17 and IFN-γ production by T cells that was similar in CD271+ and CD271- cells.
These data confirm that SF cultures contain a CD271+ cell fraction. Both CD271+ and CD271- SF cultures showed multipotential and immunomodulatory properties previously described as characteristic of MSC. T-cell anti-proliferative effect and non-specific up-regulation of T-cell cytokine production were similar in CD271+ and CD271- SF.
M. J. Del Rey,
J. D. Cañete,
J. L. Pablos,
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ACR Meeting Abstracts - https://acrabstracts.org/abstract/immunomodulatory-properties-of-cd271-and-cd271-synovial-mesenchymal-cells/