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Abstract Number: 1940

Immunologic Synovitis Score: A New Score for Synovial Membrane Characterization in Inflammatory and Non-Inflammatory Arthritis

Aurélie Najm1,2, Benoît Le Goff MD PhD2,3, Frédéric Blanchard1, Jérome Amiaud4, Céline Charrier5 and Veit Krenn6, 1INSERM U1238 University of medicine, PHY-OS Laboratory, Nantes, France, 2Rheumatology, Nantes University Hospital, Nantes, France, 3UNR1238 University of medicine, PHY-OS Laboratory, Nantes, France, 4UMR1238 University of medicine, PHY-OS Laboratory, Nantes, France, 5UNMR1238 University of medicine, PHY-OS Laboratory, Nantes, France, 6Zytologie und Molekulare Diagnostik, MVZ-Zentrum für Histologie, Trier, Germany

Meeting: 2017 ACR/ARHP Annual Meeting

Date of first publication: September 18, 2017

Keywords: rheumatoid arthritis, synovial cells, synovial fluid, Synovial Immune Biology, synovitis and synovium, synovium

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Session Information

Date: Tuesday, November 7, 2017

Session Title: Biology and Pathology of Bone and Joint Poster II

Session Type: ACR Poster Session C

Session Time: 9:00AM-11:00AM

Background/Purpose:

General Synovitis score (GSS) has been developed by Krenn et al in order to discriminate inflammatory arthritis (IA) and non-inflammatory arthritis (NIA) (1). This score allow to quantify inflammation by scoring 3 major components of synovitis: lining layer hyperplasia, activation of resident cells (stroma) and inflammatory infiltrate. All components are graded semi-quantitatively from 0 to 3 and the total score is on 9. High-grade synovitis is highly associated with IA and is defined by a score above 5 with a sensitivity of 61.7% and a specificity of 96.1%. As immunohistochemistry (IHC) is frequently used to better characterize synovitis, we propose to create a new IMmunologic SYnovitis SCore (IMSYC) adding 5 components to the GSS: CD68, CD3, CD20, CD31 and Ki67 immunostaining.

Our work aimed to evaluate the diagnostic performance of this new score including IHC, to define the best cut off for inflammatory arthritis recognition, and to compare its diagnostic performance with the GSS.

Methods:

Synovial samples from patients were obtained during surgical procedure (arthroplasty or synovectomy). All patients gave written consent prior surgery. Samples were cut and Hematoxylin and eosin stained. CD68, CD3, CD20, CD31 and KI67 IHC were performed. GSS was assessed for each slide and semi-quantitative 4 scale scores (0-3) were given for each immunostaining, in a blind manner. The score is calculated on 24 (GSS 0-9 points, and 0-3 score for each of the 5 immunostaining).

The 2 readers met and scored a representative amount of slides with a spearman correlation coefficient of 0.95 (p<0.0005). They then defined a consensual and reproducible scoring atlas.

Results:

53 patients were included. 25 were females (47,2%), mean age was 62.1 years (standard deviation (SD) 13.2 years). 36 had IA reparsed as follows: 28 Rheumatoid arthritis (RA), 5 had Psoriatic arthritis (Psa), 3 had Undifferentiated arthritis (UA). “Non inflammatory” arthritis group included 10 patients with Osteoarthritis (OA) and 7 with ligaments or meniscus injuries (post traumatic arthritis (PtA).

Mean Synovitis Score was significantly superior in the IA group 5.70 [SD 0.321] vs.3.51 [SD 0.351] ; p<0.001). Mean IMSYC was significantly superior in the IA group 14.94 [SD 0.747] vs. 8.50 [SD 0.639]; p<0.001). In univariate analysis by logistic regression, GSS (Odd Ratio (OR) 2.27; p<0.001), CD3 (OR 4.3; p=0.002), CD68 (OR 4.5; p=0.002), Ki67 (OR 11.8; p<0.001), CD31 scores (OR 6.5; p=0.001) and were significantly associated with IA, however CD20 score was not (OR 0.9; p=0.34).

ROC curve analysis of diagnostic performances determined the score of 10.5 out of 24 as the best cut off for discrimination between IA and non-IA with a sensitivity of 74.3% and specificity of 100%. The area under ROC curves (AUC) were nearly statistically different between GSS (0.81) and IMSYC (0.93) (p=0.05).

Conclusion: We hereby propose a new synovitis score including IHC. This score has a better sensitivity and specificity than the Synovitis score for discrimination between inflammatory and non-inflammatory arthritis. Moreover, IMSYC accurately describes synovial membrane immunophenotype and could therefore give a basis for tissue driven therapies in rheumatic diseases, especially in RA.


Disclosure: A. Najm, None; B. Le Goff MD PhD, None; F. Blanchard, None; J. Amiaud, None; C. Charrier, None; V. Krenn, None.

To cite this abstract in AMA style:

Najm A, Le Goff MD PhD B, Blanchard F, Amiaud J, Charrier C, Krenn V. Immunologic Synovitis Score: A New Score for Synovial Membrane Characterization in Inflammatory and Non-Inflammatory Arthritis [abstract]. Arthritis Rheumatol. 2017; 69 (suppl 10). https://acrabstracts.org/abstract/immunologic-synovitis-score-a-new-score-for-synovial-membrane-characterization-in-inflammatory-and-non-inflammatory-arthritis/. Accessed January 27, 2021.
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