Session Type: ACR Concurrent Abstract Session
Session Time: 2:30PM-4:00PM
In healthy individuals with arthralgia and RA-specific autoantibodies (so called at-risk individuals) the presence of expanded B-cell receptor (BCR) clones in peripheral blood (PB) accurately predicts who will develop arthritis in the short term1. Following up on these observations, we investigated in the same cohort of at-risk individuals whether the T-cell receptor beta (TCRβ) repertoire characteristics in PB and synovial tissue (ST) might also predict imminent onset of arthritis.
Next-Generation Sequencing of the TCRβ repertoire was performed on 20 randomly selected individuals with elevated IgM-RF and/or ACPA levels. Ten individuals did not develop RA during at least 3 years of follow-up, and 10 individuals did. Peripheral blood and synovial tissue samples were analysed during the at-risk phase and, for individuals that developed RA, again after RA onset. T-cell clones were identified by their unique TCRβ sequence2.
In the at-risk phase, the synovium is already characterized by expanded TCRβ clones, both in at-risk individuals that will and will not develop arthritis later. These clones persist in the tissue during onset of arthritis. A higher impact of the dominant TCRβ clones in the synovial tissue in the at-risk phase was associated with longer time to arthritis (p=0.02).
Expanded T-cell clones are present in the synovium of at-risk individuals regardless of future development of RA. The expanded clones are maintained after onset of clinical disease. Combined with literature data, these observations show that T cell clones are already expanded in ST very early in disease, and suggest an overall regulatory role. Further studies are needed to characterize these clones.
1Tak PP et al. Ann Rheum Dis. 2017.
2Klarenbeek PL et al. Immunol Lett. 2010.
To cite this abstract in AMA style:Balzaretti G, Klarenbeek PL, Doorenspleet ME, de Hair MJ, van Schaik BCD, Esveldt R, van de Sande M, Gerlag D, van Kampen AHC, Baas F, Tak PP, de Vries N. Expanded T-Cell Clones Are Present in the Synovium before the Clinical Onset of Rheumatoid Arthritis [abstract]. Arthritis Rheumatol. 2018; 70 (suppl 10). https://acrabstracts.org/abstract/expanded-t-cell-clones-are-present-in-the-synovium-before-the-clinical-onset-of-rheumatoid-arthritis/. Accessed November 23, 2020.
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