Session Type: ACR Poster Session B
Session Time: 9:00AM-11:00AM
Methods: HLA-DR-presented peptides were identified directly from RA patients’ synovial tissue or peripheral blood mononuclear cells (PBMC) by tandem mass spectrometry. P. copri peptides or source proteins were then tested for T cell reactivity by IFN-γ ELISpot assay and for antibody responses by ELISA using cells and sera from DMARD-naive NORA patients, chronic RA (CRA) patients, and controls. For comparison, humoral responses to P. gingivalis, B. fragilis,and E. coli were also tested. Serum samples were analyzed for innate, Th1, and Th17 mediators by Luminex. Moreover, available serum and synovial fluid (SF) samples were tested for the presence of P. copri 16S rDNA by nested PCR. All RA patients met the 2010 ACR/EULAR criteria for RA.
Results: We identified an HLA-DR-presented peptide from a 27-kD protein of
To cite this abstract in AMA style:Pianta A, Arvikar S, Strle K, Drouin EE, Wang Q, Costello CE, Steere AC. Evidence for Prevotella Copri As an Immune-Relevant Bacterium in Rheumatoid Arthritis [abstract]. Arthritis Rheumatol. 2016; 68 (suppl 10). https://acrabstracts.org/abstract/evidence-for-prevotella-copri-as-an-immune-relevant-bacterium-in-rheumatoid-arthritis/. Accessed December 1, 2020.
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ACR Meeting Abstracts - https://acrabstracts.org/abstract/evidence-for-prevotella-copri-as-an-immune-relevant-bacterium-in-rheumatoid-arthritis/