Session Type: Abstract Submissions
Session Time: 5:30PM-7:00PM
Methods: MiRNA profile was determined using Agilent Human miRNA Microarray chips using PBMC from HC and ERA, and SFMC from ERA (n=8 each). The dysregulated miRNA were validated by qRT-PCR in 25 patients and 11 healthy controls. In silico target prediction and pathway analysis was also done for these miRNAs.
Results: Eight miRNAs were differentially expressed in the PBMC from ERA patients relative to HC (p<0.25). Moreover, 90 miRNA were dysregulated (38 higher and 52 lower; p<0.05) in SFMC compared to PBMC from ERA Patients qRT-PCR validated upregulation of miR-21, miR-126, miR-130a and downregulation of miR-150 ( p<0.05) in ERA-PBMC (n=25) relative to HC-PBMC (n=11). In addition, expression of miR-155, miR-21, miR-34a, miR-210, miR-29b was upregulated and miR-146a, miR-150, miR-126, miR-130a and miR-26a was downregulated (p<0.05) in paired SFMCs relative to PBMC (n=9). Among the 4 dysregulated miRNAs in ERA-PBMC, relative to HC-PBMC, only miR-21 and miR-150 showed a positive association with their levels in SFMC (p<0.05). PDCD4, predicted target of miR-21 and miR-150, was up-regulated in SFMCs compared to ERA-PBMCs. In silico analysis revealed that the targets of differentially expressed miRNAs belong immune signaling pathways like MAPK signaling pathways, TLR signaling pathways, T cell receptor signaling pathways, mTOR signaling pathways, Wnt signaling pathways.
Conclusion: Differential expression of miR-21 and miR-150 in ERA may regulate T cell activation via PDCD4. Predicted target genes for other dysregulated miRNA found at the site of inflammation suggest a role of multiple immune pathways in ERA pathogenesis.
To cite this abstract in AMA style:Aggarwal A, Singh S. Dysregulation of miRNA in mononuclear cells of patients with enthesitis related arthritis [abstract]. Arthritis Rheumatol. 2017; 69 (suppl 4). https://acrabstracts.org/abstract/dysregulation-of-mirna-in-mononuclear-cells-of-patients-with-enthesitis-related-arthritis/. Accessed May 19, 2022.
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ACR Meeting Abstracts - https://acrabstracts.org/abstract/dysregulation-of-mirna-in-mononuclear-cells-of-patients-with-enthesitis-related-arthritis/