Distinct Balance of TNF-α/IL-10 in Circulating and Infiltrating CD163+ Macrophages between Rapidly and Slowly Progressive Symptomatic Knee Osteoarthritis

Li Wang, Wenmin Wang, Hui Chu, Xifu Shang and Xiaomei Li, Department of Rheumatology and Immunology, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China

Meeting: 2018 ACR/ARHP Annual Meeting

Keywords: cytokines, inflammation and osteoarthritis, Macrophage

Session Information

Date: Monday, October 22, 2018

Title: Osteoarthritis and Joint Biology – Basic Science Poster I

Session Type: ACR Poster Session B

Session Time: 9:00AM-11:00AM

Background/Purpose: There is a subtype of osteoarthritis (OA), which shows higher level of inflammation and higher risk of disease progression. In the current study, we analyzed the TNF-α and IL-10 level in circulating and infiltrating macrophages, and correlation of CRP and BMI between rapidly and slowly progressive patients.

Methods: We categorized 41 patients (32 female and 9 male) with symptomatic knee osteoarthritis for primary total knee arthroplasty (TKA) into rapidly progressive group（ROA, n=20）and slowly progressive group (SOA, n=22), based on disease duration less than 5 years, or more than 5 years. The average disease duration of ROA was 3.3±2.105 years, and the SOA was 12±5.385 years. 5 patients (3 female and 2 male) with knee joint fracture fixation were selected as control (FX). Circulating macrophages were separated from patients’ peripheral blood. Infiltrating macrophages were separated from knee synovial tissue from TKA. The expression of IL-10 and TNF-α in the CD163+ macrophages in the synovial membrane and peripheral blood were detected by flow cytometry. The difference of the correlation of CRP and BMI between the ROA and the SOA were analyzed.

Results: ROA patients showed significantly increased CD163+ macrophages in peripheral blood (ROA 12.53±4.56%, SOA 3.59±3.92%, FX 0.52±0.57%, p＜0.05), and even more in synovium (ROA 41.06±10.02%, SOA 20.06±3.96%, FX 0.594±0.50%, p＜0.05). CD16+ macrophages from ROA patients showed trends to TNF-α in both peripheral blood (ROA VS SOA: 42.32±6.91% VS 26.01±5.28, p＜0.05), and synovium（ROA VS SOA: 36.6±5.7% VS 23.33±4.14%, p＜0.05). However, CD16+ macrophages from SOA patients showed advantage in IL-10（peripheral blood ROS VS SOA: 2.11±2.17% VS 4.71±3.46%, p＜0.05；synovium ROS VS SOA 1.96±1.44% VS 3.80±2.33%, p＜0.05). In ROA group, CRP was positively correlated with BMI (r=0.680, P＜0.05); but in SOA group, CRP was not related to BMI.

Conclusion: Both circulating and infiltrating macrophages from patients with ROA and SOA display distinct inflammatory characters.

Disclosure: L. Wang, None; W. Wang, None; H. Chu, None; X. Shang, None; X. Li, None.

To cite this abstract in AMA style:

Wang L, Wang W, Chu H, Shang X, Li X. Distinct Balance of TNF-α/IL-10 in Circulating and Infiltrating CD163+ Macrophages between Rapidly and Slowly Progressive Symptomatic Knee Osteoarthritis [abstract]. Arthritis Rheumatol. 2018; 70 (suppl 9). https://acrabstracts.org/abstract/distinct-balance-of-tnf-%ce%b1-il-10-in-circulating-and-infiltrating-cd163-macrophages-between-rapidly-and-slowly-progressive-symptomatic-knee-osteoarthritis/. Accessed .

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