Session Information
Date: Monday, November 9, 2015
Title: Miscellaneous Rheumatic and Inflammatory Diseases Poster Session II
Session Type: ACR Poster Session B
Session Time: 9:00AM-11:00AM
Background/Purpose:
Hidradenitis suppurativa (HS) is a chronic recurrent inflammatory disease which affects 1% to 4% of young adults. The purpose of the current study was to investigate inflammatory cytokines and growth factors in the effluent from HS lesions compared to effluent from chronic wound specimens. In differentiating the inflammatory profile of HS lesions compared to chronic wounds we hope to identify potential local drivers of inflammation unique to HS over and above inflammatory responses seen in patients with chronic wounds.
Methods:
Wound fluid specimens from 8 patients with HS and 8 age-matched patients with chronic wounds were selected for analysis. Baseline demographics were obtained from the Wound Etiology and Healing (WE-HEAL) database. The Hidradenitis Suppurativa Score a validated HS disease activity score utilized in clinical trials was used to determine extent of HS activity at the time of specimen collection. The Meso Scale Discovery V-Plex cytokine panel 1 and the V-plex proinflammatory panel 1 were used to analyze proinflammatory cytokines in the fluid specimens.
Results:
There was no significant difference in race, sex, presence of diabetes, and cigarette smoking exposures in the HS compared to chronic wound cohorts. Mean HSS in the HS cohort was 68.88 (SD ±41.45) confirming moderately active disease. Interferon-gamma (IFN-γ) was significantly elevated in the HS wound fluid compared to chronic wound samples (Table 1, 1418 ± 1501 pg/ml compared to 102.5 ± 138 pg/ml, p 0.027). HS effluent also had significantly higher levels of TNF-β than chronic wound fluid (Table 1, 9.24 ± 7.22 pg/ml compared to 1.65 ± 2.14 pg/ml, p 0.03). There was no significant difference in any other proinflammatory cytokines including TNF-α, IL-1β, IL-12p70, IL-1α, IL-17A, IL-6, IL-16 and IL12/IL23p40. The anti-inflammatory cytokines IL-10, Il-4, and IL-13, the traditional proliferation cytokines, IL-2, IL-15, Il-7 and IL-5, and the growth factors GM-CSF and VEGF were not significantly different between HS effluent and chronic wound fluid.
Conclusion:
Local molecular drivers of HS are poorly understood. In this study we were able to show that when compared with effluent from chronic wounds HS effluent contains higher levels of the cytokines IFN-γ and TNF-β suggesting that IFN-γ and TNF-β may play a crucial role in driving and perpetuating the exuberant inflammation in HS.
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|
WOUND FLUID |
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|
HIDRADENITIS (pg/mL) N=8 Mean ±SD |
CHRONIC WOUND (pg/mL) N=8 Mean ±SD |
P value |
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|
|
AGE |
41.61 ± 13.81 |
45.32 ± 10.43 |
0.55 |
NS |
|
PRO-INFLAMMATORY |
INF-γ |
1418 ± 1501 |
102.5 ± 138 |
0.027 |
* |
|
IL-12p70 |
9.412 ± 7.59 |
15.02 ± 27.28 |
0.609 |
NS |
|
|
IL-1β |
862.5 ± 1076 |
1503 ± 3500 |
0.69 |
NS |
|
|
IL-1α |
1126 ± 1746 |
2549 ± 5565 |
0.5291 |
NS |
|
|
IL-17A |
1006 ± 1652 |
32.7 ± 37.36 |
0.12 |
NS |
|
|
IL-6 |
2377 ± 1604 |
5451 ± 8180 |
0.32 |
NS |
|
|
TNF-α |
83.26 ± 69.07 |
65.74 ± 105.4 |
0.70 |
NS |
|
|
TNF-β |
9.24 ± 7.22 |
1.65 ± 2.14 |
0.03 |
* |
|
|
IL-16 |
15277 ± 18785 |
15586 ± 25553 |
0.97 |
NS |
|
|
IL12/IL23p40 |
488.3 ± 570.8 |
97.86 ± 67.06 |
0.07 |
NS |
|
|
ANTI-INFLAMMATORY |
IL-10 |
19.85 ± 18.74 |
34.74 ± 69.58 |
0.57 |
NS |
|
IL-4 |
6.56 ± 4.15 |
9.77 ± 13.98 |
0.54 |
NS |
|
|
IL-13 |
70.98 ± 48.16 |
55.61 ± 56.10 |
0.56 |
NS |
|
|
PROLIFERATION |
IL-2 |
18.97 ± 18.66 |
13.16 ± 22.15 |
0.60 |
NS |
|
IL-15 |
24.5 ± 37.29 |
5.61 ± 4.75 |
0.18 |
NS |
|
|
IL-7 |
22.29 ± 20.49 |
10.45 ± 6.28 |
0.14 |
NS |
|
|
IL-5 |
30.15 ± 33.63 |
9.314 ± 18.01 |
0.17 |
NS |
|
|
GROWTH FACTOR |
GM-CSF |
78.45 ± 62.6 |
82.13 ± 208.8 |
0.96 |
NS |
|
VEGF |
632.1 ± 757.4 |
1544 ± 1765 |
0.23 |
NS |
|
Table 1: Comparison of cytokines profiles in HS and chronic wounds
To cite this abstract in AMA style:
Banerjee A, Shanmugam VK, McNish S, Couch K. Differential Expression of Interferon Gamma in Exudate from Hidradenitis Suppurativa Lesions Compared to Chronic Wounds [abstract]. Arthritis Rheumatol. 2015; 67 (suppl 10). https://acrabstracts.org/abstract/differential-expression-of-interferon-gamma-in-exudate-from-hidradenitis-suppurativa-lesions-compared-to-chronic-wounds/. Accessed .« Back to ACR/ARHP Annual Meeting 2015
ACR Meeting Abstracts - https://acrabstracts.org/abstract/differential-expression-of-interferon-gamma-in-exudate-from-hidradenitis-suppurativa-lesions-compared-to-chronic-wounds/