Date: Monday, November 6, 2017
Session Title: T Cell Biology and Targets in Autoimmune Disease Poster I
Session Type: ACR Poster Session B
Session Time: 9:00AM-11:00AM
Background/Purpose: To compare microarray gene analysis of patients with Giant cell arteritis to patients with Takayasu arteritis.
Methods: We performed comparative microarray gene analysis of purified CD4+ T cells of TA (n=14) and GCA (n=15) patients.
Results: We found that purified CD4+ T cells of GCA and TA patients exhibit distinct RNA signatures. Sixty-seven genes were differentially expressed, among which CXCR5, CCR6 and CCL20 were shown to be significantly up-regulated in CD4+ T cells of TA patients as compared to those of GCA. Flow cytometry analysis confirmed that CXCR5+ CD4+ cells, defined as T Follicular Helper cells (TFH), were significantly higher in TA patients as compared to GCA and healthy donors (HD) [median proportion of CXCR5+ CD4+ T cells of 15.4 (10;30.8) in TA versus 5.3 (1.4; 12.2) in GCA (p<0.0001) and 9.7 (5.6; 12.5) in HD (p=0.0001)]. Among TFH subpopulations, TFH-17 (defined as CCR6+ CXCR5+ CD4+ T cells) was specifically increased in TA [4.52 (1.07; 13.35) in TA vs 0.69 (0.13; 2.16) in GCA (p=0.0001) and 2.3 (0.3; 4.2) in HD (p=0.02)]. The analysis of inflammatory aortic lesions revealed the presence of tertiary lymphoid structures, composed of CXCR5+, CD4+, PD-1 and CD-20 cells in TA whereas such organized inflammatory infiltrates were uncommon in GCA aortic lesions. We demonstrated an increased peripheral B cell immune response in TA, as the number of peripheral B cells is increased in TA compared to GCA. CXCR5+ CD4+ T cells in TA patients have specific characteristics of TFH, as they help B cells to differentiate into memory B cells, to proliferate and to secrete IgG.
Conclusion: Patients with TA differ from patients with GCA in microarray gene analysis of purified CD4+ T cells. Our data provide evidence of the critical role of TFH in pathogenesis of inflammatory lesions in TA and suggest the role of antigenic trigger.
To cite this abstract in AMA style:Desbois AC, Quiniou V, Bruneval P, Derian N, Maciejewski-Duval A, Garrido M, Comarmond C, Pouchot J, Rosenzwajg M, Klatzmann D, Cacoub P, Saadoun D. CXCR5+ CD4 T Cells Signature Differentiates Takayasu Arteritis from Giant Cell Arteritis [abstract]. Arthritis Rheumatol. 2017; 69 (suppl 10). https://acrabstracts.org/abstract/cxcr5-cd4-t-cells-signature-differentiates-takayasu-arteritis-from-giant-cell-arteritis/. Accessed November 25, 2020.
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ACR Meeting Abstracts - https://acrabstracts.org/abstract/cxcr5-cd4-t-cells-signature-differentiates-takayasu-arteritis-from-giant-cell-arteritis/