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Abstract Number: 811

Basophils Are Activated and Stimulate Both B Cells and Fibroblasts in Systemic Sclerosis

Benjamin Chaigne1, Nicolas Dumoitier2, Alexis Regent1, Benjamin Terrier3, Jonathan London2, Matthieu Groh1, Nathalie Thieblemont4 and Luc Mouthon5, 1National Referral Center for Rare Systemic Autoimmune Diseases, Hôpital Cochin, AP–HP, Université Paris Descartes, Paris, France, 2INSERM U1016, Institut Cochin, Equipe Neutrophiles et Vascularites, Paris, France, 3Internal Medicine, Cochin Hospital, Paris, France, 4Inserm U1016, Paris, France, 5Department of Internal Medicine, Referral Center for Rare Autoimmune and Systemic Diseases, Hôpital Cochin, AP–HP, Université Paris Descartes, Paris, France, Paris, France

Meeting: 2016 ACR/ARHP Annual Meeting

Date of first publication: September 28, 2016

Keywords: B cells, basic research, fibrosis, innate immunity and systemic sclerosis

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Session Information

Date: Sunday, November 13, 2016

Session Title: Systemic Sclerosis, Fibrosing Syndromes and Raynaud's – Pathogenesis, Animal Models and Genetics - Poster I

Session Type: ACR Poster Session A

Session Time: 9:00AM-11:00AM

Background/Purpose: Systemic sclerosis (SSc) is a rare multisystem connective tissue disease characterized by skin and internal organs fibrosis and vascular abnormalities, along with the presence of autoantibodies. Basophils, which have long been associated with allergy and parasitic infections, are now identified as having the ability to promote fibrosis, stimulate B cells and increase autoantibodies production. Herein we investigated the potential role of basophils in SSc.

Methods: Peripheral basophils from patients with SSc who did not receive glucocorticoids or immunosuppressants and healthy controls (HC) were analyzed using flow cytometry and basophil activation test. The impact of the KU812F basophil cell line on both B cells and fibroblasts isolated from SSc patients were assessed using flow cytometry and xCelligence assay.

Results: Sixty-five consecutive SSc patients and 38 HC were recruited. The proportion of CD203c-positive basophils was higher in SSc patients than in HC (37.5 % [24.3 – 59.0] vs 25.6% [13.6 – 41.7]; p < 0.001). Compared to HC, basophils from SSc patients had higher mean fluorescence intensity (MFI) quantifications of CD154 (854 [541 – 1334] vs 485 [147 – 871]; p < 0.05) and intracellular B cell activating factor (1551 [1001 – 4373] vs 857 [722 – 1498]; p < 0.05). Activated KU812F basophils were able to decrease the proportion of CD95-positive B cells (10.5% [8.4 – 12.6 vs 14.8% [14.4 – 21.5]; p < 0.05), to increase the production of interleukine-6 (21.2% [16.5 – 29.9] vs 14.9 [12.3– 16.7]; p < 0.05) and transforming growth factor-β (5.6% [4.7 – 7.2] vs 4.1 [2.6– 5.4]; p < 0.05) producing B cells and to enhance the proliferation of fibroblasts from patients with SSc. Lastly, the expression of CRTH2 on basophils correlated with patients’ disease characteristics and was able to discriminate those with or without pulmonary hypertension (PAH). Indeed, CRTH2 MFI quantification among basophils positively correlated with patients’ diffusing capacity of the lungs for carbon monoxide (r = 0.60, p < 0.05), negatively correlated with patients’ modified Rodnan skin score (r = – 0.77, p < 0.01) and was lower in SSc patients with PAH than in SSc patients without PAH (31476 [30213 – 31633] vs 59576 [41312 – 67788]; p < 0.001).

Conclusion: Peripheral basophils are activated and are able to simulate both B cells and fibroblasts in patients with SSc, suggesting a key role of basophils in the pathophysiology of SSc.


Disclosure: B. Chaigne, None; N. Dumoitier, None; A. Regent, None; B. Terrier, None; J. London, None; M. Groh, None; N. Thieblemont, None; L. Mouthon, None.

To cite this abstract in AMA style:

Chaigne B, Dumoitier N, Regent A, Terrier B, London J, Groh M, Thieblemont N, Mouthon L. Basophils Are Activated and Stimulate Both B Cells and Fibroblasts in Systemic Sclerosis [abstract]. Arthritis Rheumatol. 2016; 68 (suppl 10). https://acrabstracts.org/abstract/basophils-are-activated-and-stimulate-both-b-cells-and-fibroblasts-in-systemic-sclerosis/. Accessed May 19, 2022.
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