ACR Meeting Abstracts

ACR Meeting Abstracts

Abstract Number: 0065

Altered Expression of Several miRNAs in Axial Spondyloarthritis Patients with High Disease Activity

Jiri Baloun1, Kristyna Bubova1, Aneta Pekacova1, Monika Gregova2, Sarka Forejtova1, Jana Horinkova1, Michal Tomcik3, Jindriska Gatterova1, Jiri Vencovsky1, Karel Pavelka3 and Ladislav Senolt3, 1Institute of Rheumatology, Prague, Czech Republic, 2Rheumatology Institute Prague, Prague, Czech Republic, 3Institute of Rheumatology, Department of Rheumatology, 1st Faculty of Medicine, Charles University, Prague, Czech Republic

Meeting: ACR Convergence 2021

Keywords: , , ,

Session Information

Date: Saturday, November 6, 2021

Session Title: Spondyloarthritis Including PsA – Basic Science Poster (0046–0068)

Session Type: Poster Session A

Session Time: 8:30AM-10:30AM

Background/Purpose: Recent studies have shown that microRNAs (miRNAs) play a role in various disorders, including immune mediated inflammatory diseases. Therefore, we studied relationship between miRNA profiles, disease activity and selected clinical manifestations of axial spondyloarthritis (axSpA).

Methods: We studied the whole miRNome in peripheral blood mononuclear cells (PBMC) of patients with non-radiographic (n=38) and radiographic (n=38) axSpA. The miRNA expression was profiled by massive parallel sequencing. The expression of particular miRNAs was correlated with disease activity (ASDAS, BASDAI and CRP) and selected clinical manifestations (e.g. uveitis). Sequencing data were analysed using DESeq2 algorithm, which additionally assessed the relationship of miRNA expression with BASDAI or the presence of uveitis, and the relationship with ASDAS and CRP were analysed using generalized linear modelling with negative binomial assumption (GLM-NB).

Results: Although DESeq2 did not reveal associations between miRNA expression, BASDAI or uveitis; the expression of several miRNAs correlated with the levels of CRP (p < 0.05). The pairwise comparison of ASDAS levels by Dunnett`s test revealed that 14 miRNAs were significantly different in axSpA patients with high and very high disease activity compared to patients with inactive disease. The pathway enrichment analysis by MIENTURNET revealed a list of pathways, with emphasis on interleukin (e.g., IL-4, IL-6, IL-10), JAK-STAT and NOTCH signalling pathways, in which dysregulated miRNAs are potentially involved. Additionally, the screening revealed that miR-1246 was differently expressed between radiographic and non-radiographic axSpA patients.

Conclusion: Our results revealed altered expression of several miRNAs in patients with high disease activity of axSpA, which may affect their target genes associated with pathogenesis of the disease.

This work was supported by the project MHCR 023728 and AZV No. 17-33127A S.

Disclosures: J. Baloun, None; K. Bubova, None; A. Pekacova, None; M. Gregova, None; S. Forejtova, None; J. Horinkova, None; M. Tomcik, None; J. Gatterova, None; J. Vencovsky, AbbVie, 1, 6, Boehringer Ingelheim, 2, Eli Lilly, 1, 6, Gilead, 1, Octapharma, 1, Biogen, 6, MSD, 6, Pfizer, 6, Roche, 6, Sanofi, 6, UCB, 6, Novartis, 6, Werfen, 6; K. Pavelka, Abbvie, 6, UCB, 6, MSD, 6, Roche, 6, Pfizer, 6, Eli Lilly, 6, Egis, 6, Biogen, 6, Pfizer, 6; L. Senolt, None.

To cite this abstract in AMA style:

Baloun J, Bubova K, Pekacova A, Gregova M, Forejtova S, Horinkova J, Tomcik M, Gatterova J, Vencovsky J, Pavelka K, Senolt L. Altered Expression of Several miRNAs in Axial Spondyloarthritis Patients with High Disease Activity [abstract]. Arthritis Rheumatol. 2021; 73 (suppl 9). https://acrabstracts.org/abstract/altered-expression-of-several-mirnas-in-axial-spondyloarthritis-patients-with-high-disease-activity/. Accessed January 30, 2023.

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ACR Meeting Abstracts - https://acrabstracts.org/abstract/altered-expression-of-several-mirnas-in-axial-spondyloarthritis-patients-with-high-disease-activity/