Session Information
Title: T-cell Biology and Targets in Autoimmune Disease: Signaling Pathways in T-cell Differentiation
Session Type: Abstract Submissions (ACR)
Background/Purpose:
To investigate a role of Adiponectin (AD) on the differentiation of naïve T cell to Th17 cell and bone erosion in collagen-induced arthritis (CIA) mice.
Methods:
Purified naïve T cells were cultured in anti-CD3 mAb and anti-CD28 mAb precoated with TGF-β, IL-6, IL-23 in the presence and absence of AD (0.1, 1 or 10 μg/ml). After 72 hours of culture, the frequencies of Th17 cells were measured by flow cytometry; the expression of ROR-γt and IL-17 were determined by real-time PCR and ELISA. Intraarticularly injected of AD 10 μl (1 μg/1 μl) into the knee joint of CIA mice was to analysis the role of AD on CIA development and Th17 expression in vivo.
Results:
The frequencies of Th17 cells were significantly increased with the concentration of AD 10 μg/ml, but AD treatment with 0.1 and 1 μg/ml showed no obvious effect on the differentiation of naïve T cell to Th17 cell. The expression of IL-17 and ROR-γt mRNA were significantly increased oupon AD 0.1 μg/ml and 10 μg/ml AD stimulation. Consistently with mRNA expression, IL-17 levels were significantly elevated in culture supernatants after 10 μg/ml AD treatment. Intraarticular injection of AD into the knee joint of CIA mice aggravated arthritic development and bone erosion, triggered higher expression of IL-17 mRNA and its transcription factor including ROR-γt, IL-21, IL-22 and IL-23 in CIA model.
Conclusion:
These findings identify a role of AD on enhancing the differenation of naïve T cell to Th17 cell, contributed to CIA bone erosion in CIA mice.
Disclosure:
M. Zhang,
None;
X. Sun,
None;
W. Tan,
None;
X. Feng,
None;
K. Gan,
None.
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ACR Meeting Abstracts - https://acrabstracts.org/abstract/adiponectin-promotes-the-differentiation-of-naive-t-cell-to-th17-cell-and-aggravates-collagen-induced-arthritis/