ACR Meeting Abstracts

ACR Meeting Abstracts

  • Home
  • Meetings Archive
    • ACR Convergence 2020
    • 2020 ACR/ARP PRSYM
    • 2019 ACR/ARP Annual Meeting
    • 2018 ACR/ARHP Annual Meeting
    • 2017 ACR/ARHP Annual Meeting
    • 2017 ACR/ARHP PRSYM
    • 2016-2009 Meetings
    • Download Abstracts
  • Keyword Index
  • Advanced Search
  • Your Favorites
    • Favorites
    • Login
    • Register
    • View and print all favorites
    • Clear all your favorites
  • Meeting Resource Center

Abstract Number: 2445

A Distinct Profile of Circulating Microparticles Is Associated with Disease Features in Rheumatoid Arthritis Patients and Impairs Endothelial Functionality in Vitro

Javier Rodríguez-Carrio1, Mercedes Alperi-López2, Patricia López1, Sara Alonso-Castro2, Santiago Rubén Carro-Esteban1, Javier Ballina-García2 and Ana Suárez1, 1Area of Immunology, Department of Functional Biology, University of Oviedo, Oviedo, Spain, 2Rheumatology Department, Hospital Universitario Central de Asturias, Oviedo, Spain

Meeting: 2014 ACR/ARHP Annual Meeting

Keywords: Cardiovascular disease, endothelial cells, microparticles and rheumatoid arthritis (RA)

  • Tweet
  • Email
  • Print
Save to PDF
Session Information

Session Title: Rheumatoid Arthritis - Human Etiology and Pathogenesis

Session Type: Abstract Submissions (ACR)

Background/Purpose

Cell-derived microparticles (MPs) could be considered biomarkers of cell damage and activation and they are thought to have a role in cardiovascular (CV) and inflammatory diseases. Since Rheumatoid Arthritis (RA) is characterized by immune and endothelial activation, the main aim of this study was to evaluate MP counts in RA patients.

Methods

MPs were analyzed by flow cytometry following a total-labelling procedure in platelet-poor plasma from 33 healthy controls (HC), 72 individuals with marked CV risk (CVR: diabetes, n=24; dyslipidemia, n=27; and hypertension, n=41) and 114 RA patients (61.4% RF, 61.4% αCCP, DAS28: 3.61(1.97), 42.1% erosive disease). Different subsets were identified by their surface markers: platelet- (CD41+, PMPs), endothelial- (CD146+, EMPs), granulocyte- (CD66+, GMPs), monocyte- (CD14+, MoMPs) and Tang-derived (CD3+CD31+, Tang-MPs). TNFα serum levels were quantified by ELISA. In vitro assays on Matrigel with HMEC-I cells were performed to assess the effect of MPs on endothelial functionality. Clinical and immunological parameters as well as traditional CV risk factors (diabetes, hypertension, dyslipidemia, obesity and smoking) were registered from clinical records and all data were analyzed by Principal Component Analysis (PCA).

Results

Total MPs count was increased in RA compared to both HC (p<0.0001) and CVR (p=0.0009) and was positively correlated with traditional CV risk factors (BMI, TC/HDL ratio, triglycerides and number of risk factors). Additionally, specific MPs subsets were increased in RA (EMPs p<0.0001, GMPs p<0.0001, Tang-MPs p=0.006 and MoMPs p=0.028). Clinical data were integrated with PCA and 4 components were identified. Notably, different MP subsets correlated with different components (table 1), thereby involving specific disease features in MPs profile. Interestingly, TNFα correlated with Tang-MPs in RA after adjusting by traditional risk factors (r=0.218, p=0.036). Finally, MPs from RA patients were able to impair endothelial cell functionality (measure as tube formation and number of branching points) in vitro in a dose-dependent manner, linked to an upregulation of endothelial activation markers (CD62E, CD144 and VEGFR2). This effect appeared even within the physiological range and it was not present with HC or CVR MPs.

Conclusion

MPs analysis in RA patients revealed an increased damage in several cell types. Circulating MPs from RA patients displayed a unique quantitative and qualitative profile as the result of both disease-specific and traditional CV risk factors. These differences are independent of comorbidities. Accordingly, this MP profile could underlie the detrimental effects on endothelial cells in vitro, thus supporting their role as biomarkers of endothelial damage and vascular repair failure.

Table 1: PCA components

 

Rheumatic-related

Traditional CV-related

Duration-related

Inflammation-related

Total MPs

0.110

0.345

0.381

0.0007 ***

0.065

0.576

0.022

0.848

PMPs

0.018

0.881

0.078

0.506

0.096

0.410

0.050

0.668

EMPs

-0.016

0.894

0.017

0.884

0.302

0.005 **

-0.091

0.446

GMPs

0.277

0.022 *

-0.004

0.975

0.042

0.721

0.050

0.667

Tang-MPs

0.299

0.003 **

-0.035

0.768

-0.028

0.816

0.134

0.262

MoMPs

-0.098

0.414

-0.111

0.356

-0.153

0.202

0.114

0.343


Disclosure:

J. Rodríguez-Carrio,
None;

M. Alperi-López,
None;

P. López,
None;

S. Alonso-Castro,
None;

S. R. Carro-Esteban,
None;

J. Ballina-García,
None;

A. Suárez,
None.

  • Tweet
  • Email
  • Print
Save to PDF

« Back to 2014 ACR/ARHP Annual Meeting

ACR Meeting Abstracts - https://acrabstracts.org/abstract/a-distinct-profile-of-circulating-microparticles-is-associated-with-disease-features-in-rheumatoid-arthritis-patients-and-impairs-endothelial-functionality-in-vitro/

Advanced Search

Your Favorites

You can save and print a list of your favorite abstracts by clicking the “Favorite” button at the bottom of any abstract. View your favorites »

ACR Convergence: Where Rheumatology Meets. All Virtual. November 5-9.

ACR Pediatric Rheumatology Symposium 2020

© COPYRIGHT 2021 AMERICAN COLLEGE OF RHEUMATOLOGY

Wiley

  • Home
  • Meetings Archive
  • Advanced Search
  • Meeting Resource Center
  • Online Journal
  • Privacy Policy
  • Permissions Policies
loading Cancel
Post was not sent - check your email addresses!
Email check failed, please try again
Sorry, your blog cannot share posts by email.
This site uses cookies: Find out more.