ACR Meeting Abstracts

ACR Meeting Abstracts

Abstract Number: 1813

A Comprehensive Method to Study Environmental Chemicals in Serum in Patients with SLE

Cristina Lanata1, Kim Taylor2, James Hurst-Hopf1, Laura Trupin1, Patricia Katz3, Jinoos Yazdany1, Maria Dall'Era4, Dimitri Abrahamsson1, Lindsey Criswell5 and Roy Gerona1, 1UCSF, San Francisco, CA, 2University of California, San Francisco, San Francisco, CA, 3University of California, San Francisco, Novato, CA, 4Division of Rheumatology, University of California, San Francisco, CA, 5Rosalind Russell/Ephraim P. Engleman Rheumatology Research Center, University of California San Francisco, San Francisco, CA

Meeting: ACR Convergence 2020

Keywords: ,

Session Information

Date: Monday, November 9, 2020

Session Title: SLE – Diagnosis, Manifestations, & Outcomes Poster III: Bench to Bedside

Session Type: Poster Session D

Session Time: 9:00AM-11:00AM

Background/Purpose: Environmental exposures may play a substantial role in the pathogenesis of Systemic Lupus Erythematosus (SLE), however it has been challenging to measure and capture relevant exposures in a comprehensive manner. The goal of this study is to develop a novel method to characterize the serum levels of circulating environmental chemicals and metabolites in a well-characterized cohort of SLE patients and controls.

Methods: Participants from the California Lupus Epidemiology Study (CLUES), a longitudinal cohort of individuals with rheumatologist-confirmed SLE were included (n=332). A cohort of healthy individuals matched by age, ethnicity and sex was recruited (n=100).  Serum was analyzed by liquid chromatography quadruple time-of-flight Mass Spectrometry (LC-QTOF/MS). Feature detection, peak alignment, and abundance normalization using internal standards was performed using Agilent Mass Profiler  and adjusted for batch effects using ComBat1.Principal Components Analysis was used to assess batch adjustment and correct for residual batch effects. Compounds detected in at least 25% of participants were selected for association testing between chemical abundances and SLE status. We analyzed differences between cases and controls using linear models of feature abundances, adjusting for censoring below the detection limit, residual batch effects, and covariates age, sex, and ethnicity. Results of potential detected environmental chemicals were matched into a curated database of hazardous environmental chemicals.

1Johnson et al, Biostatistics. 2007; 8(1):118–27

Results: We detected 66,861 features in our cohort; with 3,192 present in at least 25% of samples and analyzed for association with case status.   Out of the 3192 features, 70 were significantly different, including 4-Hexyloxyphenol and 39 additional formula-matched compounds.

Conclusion: LC-QTOF/MS can identify a wider range of potential chemical exposures in SLE, and may aid in prioritizing chemicals for further research and intervention.  We found that patients with SLE had different chemicals and endogenous metabolites compared to healthy controls.

Disclosure: C. Lanata, None; K. Taylor, None; J. Hurst-Hopf, None; L. Trupin, None; P. Katz, None; J. Yazdany, Eli Lilly, 5, Astra Zeneca, 5; M. Dall'Era, Janssen, 5, AstraZeneca, 5; D. Abrahamsson, None; L. Criswell, None; R. Gerona, None.

To cite this abstract in AMA style:

Lanata C, Taylor K, Hurst-Hopf J, Trupin L, Katz P, Yazdany J, Dall'Era M, Abrahamsson D, Criswell L, Gerona R. A Comprehensive Method to Study Environmental Chemicals in Serum in Patients with SLE [abstract]. Arthritis Rheumatol. 2020; 72 (suppl 10). https://acrabstracts.org/abstract/a-comprehensive-method-to-study-environmental-chemicals-in-serum-in-patients-with-sle/. Accessed May 19, 2022.

« Back to ACR Convergence 2020

ACR Meeting Abstracts - https://acrabstracts.org/abstract/a-comprehensive-method-to-study-environmental-chemicals-in-serum-in-patients-with-sle/