Date: Monday, November 9, 2020
Session Type: Poster Session D
Session Time: 9:00AM-11:00AM
Background/Purpose: Environmental exposures may play a substantial role in the pathogenesis of Systemic Lupus Erythematosus (SLE), however it has been challenging to measure and capture relevant exposures in a comprehensive manner. The goal of this study is to develop a novel method to characterize the serum levels of circulating environmental chemicals and metabolites in a well-characterized cohort of SLE patients and controls.
Methods: Participants from the California Lupus Epidemiology Study (CLUES), a longitudinal cohort of individuals with rheumatologist-confirmed SLE were included (n=332). A cohort of healthy individuals matched by age, ethnicity and sex was recruited (n=100). Serum was analyzed by liquid chromatography quadruple time-of-flight Mass Spectrometry (LC-QTOF/MS). Feature detection, peak alignment, and abundance normalization using internal standards was performed using Agilent Mass Profiler and adjusted for batch effects using ComBat1.Principal Components Analysis was used to assess batch adjustment and correct for residual batch effects. Compounds detected in at least 25% of participants were selected for association testing between chemical abundances and SLE status. We analyzed differences between cases and controls using linear models of feature abundances, adjusting for censoring below the detection limit, residual batch effects, and covariates age, sex, and ethnicity. Results of potential detected environmental chemicals were matched into a curated database of hazardous environmental chemicals.
1Johnson et al, Biostatistics. 2007; 8(1):118–27
Results: We detected 66,861 features in our cohort; with 3,192 present in at least 25% of samples and analyzed for association with case status. Out of the 3192 features, 70 were significantly different, including 4-Hexyloxyphenol and 39 additional formula-matched compounds.
Conclusion: LC-QTOF/MS can identify a wider range of potential chemical exposures in SLE, and may aid in prioritizing chemicals for further research and intervention. We found that patients with SLE had different chemicals and endogenous metabolites compared to healthy controls.
To cite this abstract in AMA style:Lanata C, Taylor K, Hurst-Hopf J, Trupin L, Katz P, Yazdany J, Dall'Era M, Abrahamsson D, Criswell L, Gerona R. A Comprehensive Method to Study Environmental Chemicals in Serum in Patients with SLE [abstract]. Arthritis Rheumatol. 2020; 72 (suppl 10). https://acrabstracts.org/abstract/a-comprehensive-method-to-study-environmental-chemicals-in-serum-in-patients-with-sle/. Accessed June 21, 2021.
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ACR Meeting Abstracts - https://acrabstracts.org/abstract/a-comprehensive-method-to-study-environmental-chemicals-in-serum-in-patients-with-sle/