Anti-Inflammatory Effect Of Resveratrol As a Dietary Supplement In An Antigen-Induced Arthritis Rat Model

Romina R. Riveiro-Naveira¹, Jesus Loureiro¹, Alberto Centeno-Cortés², Eduardo López-Peláez², Carlos Vaamonde-García¹, M. Noa Valcárcel-Ares¹, Francisco J. Blanco³ and Maria J. López-Armada¹, ¹Aging and Inflammation Research Lab, INIBIC-CHU A Coruña, A Coruña, Spain, ²Experimental Surgery Unit, CHU A Coruña, A Coruña, Spain, ³Osteoarticular and Aging Research Laboratory, Proteomics Unit-Associated Node to ProteoRed-ISCIII, INIBIC-CHUAC, A Coruña, Spain

Meeting: 2013 ACR/ARHP Annual Meeting

Keywords: Animal models, immune response, Inflammation, resveratrol and synovial cells, synovial fluid

Session Information

Title: Rheumatoid Arthritis - Animal Models I

Session Type: Abstract Submissions (ACR)

Background/Purpose: Inflammatory cells like macrophages, lymphocytes and neutrophils in hyperplasic synovial tissue have been identified as key players in the onset and development of rheumatoid arthritis (RA). Resveratrol (RSV) is a natural antioxidant with potent immunomodulatory and anti-inflammatory properties. The aim of this study was to investigate the preventive effect of dietary resveratrol and its ability to suppress inflammatory arthritis progression in an acute antigen-induced arthritis (AIA) rat model.

Methods: Six-week-old female Lewis rats were randomized into four study groups: healthy group (n=19), AIA group (n=24), RSV group (n=26) and diclofenac (DCF) group (n=17). RSV (2 mg/day) was administered daily by oral gavage as a dietary supplement beginning one month before AIA induction until sacrifice day, 48h after intraarticular injection. DCF (1,2 mg/day) was orally administered three days before AIA induction until sacrifice day. Joint swelling was assessed by measuring the mediolateral diameter of both right and left knee joints with a digital calliper. Severity of inflammation was evaluated using a blind damage scoring system (H&E). To characterize inflammatory cell infiltration and chemokine levels, CD68, CD3 and MCP-1 expression were evaluated by immunohistochemistry.

Results: On day 2 after intraarticular injection, RSV animals exhibited significant reduced knee swelling, similar to DCF group, in comparison to AIA animals (mediolateral diameter mean: RSV 7,775 mm ± 0,169, n=26; DCF 7,776 mm ± 0,253, n=17 vs. AIA 8,281 ± 0,127, n=24; p<0,05). Accordingly, histopatological evaluation demonstrated a reduction in synovial hyperplasia and cell infiltration in both RSV and DCF groups compared with AIA animals. CD68 and CD3 infiltrate was significantly attenuated in RSV rats synovium (0,843 ± 0,369, n=5 and 1,290 ± 0,198, n=19, respectively) compared with AIA group (CD68 3,741 ± 1,428, n=5 and CD3 2,868 ± 0,5707, n=18, p<0,05), similar to DCF group (CD68 1,656 ± 0,522, n=13 and CD3 1,753 ± 0,359, n=15). MCP1 levels in the synovium were significantly attenuated in RSV and DCF groups compared with AIA animals (3,167 ± 0,2649 and 2,453 ± 0,628, respectively vs. AIA 4,400 ± 0,4922, p<0,05).

Conclusion: The results of this study suggest that a dietary supplement with the natural antioxidant resveratrol can effectively control inflammation and could exert a protective effect against RA establishment and progression.

Disclosure:

R. R. Riveiro-Naveira,
None;

J. Loureiro,
None;

A. Centeno-Cortés,
None;

E. López-Peláez,
None;

C. Vaamonde-García,
None;

M. N. Valcárcel-Ares,
None;

F. J. Blanco,
None;

M. J. López-Armada,
None.

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