Session Information
Date: Tuesday, November 10, 2015
Title: Vasculitis Poster III
Session Type: ACR Poster Session C
Session Time: 9:00AM-11:00AM
Background/Purpose: Interleukin-17 (IL-17) has been associated with the pathogenesis of various inflammatory diseases. The aim of our study was to investigate the expression of Th17-related immunity in two phenotypically different disorders: Behcet’s disease (BD) with innate responses and Takayasu’s arteritis (TAK) with adaptive immunity.
Methods: The study population consisted of three groups: patients with BD (n=37, age: 39.2 ±10.1 years), patients with TAK (n=25, age: 50.9 ±15.5 years) and healthy controls (HC) (n=25, age 40.9± 6.9 years). Peripheral blood mononuclear cells (PBMC) from patients with BD, TAK and HC were cultured in Th17 inducing condition (IL-6, PHA, IL-1b and IL-23) for 6 days. Cultured cells were then stained with CD4, CD8, CD3, TCRgd, CD19 IFN-ɣ and IL-17 antibodies to determine the intracellular cytokine secretion by flow cytometry.
Results: In BD patients, IL-17 expression by CD4+ T cells was observed to be higher than both TAK patients and HC (8.9±10.6 vs 3.5±2.5 and 4.5±4.9, p=0.03 and p=0.02, respectively). IL-17 expression by gd+ (14.4±13.3 vs 6.3±4.4 and 6.1±4.1, p=0.001, p<0.001 respectively) and CD8+T cells (7.0±8.3 vs 3.6±3.8 and 2.3±2.1, p=0.007 and p<0.001, respectively) was also higher in BD compared to TAK and HC. No differences were observed between the groups in the IL-17 production by B cells. Under Th17 inducing conditions, production of IFN-ɣ by CD4+ T cells (4.8±5.2 vs 2.0±1.7 and 1.9±1.3, p<0.05) and CD8 +T cells (11.3±12.6 vs 4.3±4.4 and 4.0±3.6, p<0.05) were also higher in both BD and TAK patients compared to the HC. No differences were observed between the groups in IFN-ɣ production by B cells and gd+ T cells. IL-17 expression and IFN-ɣ production in patients with BD and TAK under immuno-suppressive (IS) treatments were not different compared to patients without IS and HC (Table 1).
Conclusion: Our preliminary results suggest that under Th17 stimulating conditions, T cells express higher IL-17 levels in BD. More prominent IL-17 and IFN-ɣ production by all lymphocyte subsets in BD might be associated with the increased innate responses and early tissue neutrophil infiltrations in BD, which is not observed in TAK.
Table 1. IL-17 expression and IFN-ɣ production in HC and BD and TAK patients with and without IS treatment
|
|
HC |
IS BD |
Non-IS BD |
IS TA |
Non-IS TA |
|
CD4+ IFN+ |
1.9±1.3 |
2.6±0.7 |
3.9±4.3 |
2.2±1.9 |
1.6±1.2 |
|
CD4+ IL-17+ |
4.5±4.9 |
7.8±8.5 |
8.9±11.3 |
3.3±2.3 |
3.5±3.1 |
|
CD8+ IFN+ |
4.0±3.6 |
6.1±2.6 |
10.2±12.1 |
5.2±5.0 |
3.3±3.1 |
|
CD8+ IL-17+ |
2.3±2.1 |
8.4±5.3 |
7.1±9.4 |
4.0±4.5 |
3.2±2.4 |
|
Gd+ IFN+ |
5.3±4.7 |
10.0±5.4 |
10.7±12.8 |
4.5±4.6 |
6.3±6.3 |
|
Gd+ IL17+ |
6.1±4.1 |
13.3±9.9 |
15.4±14.0 |
6.9±4.9 |
5.2±3.7 |
To cite this abstract in AMA style:
Unal AU, Deniz R, Tulunay Virlan A, Ture Ozdemir F, Aydin Tatli I, Ozen G, Alibaz-Oner F, Mumcu G, Ergun T, Direskeneli H. IL-17 Expression By Lymphocytes Is Higher in Behcet’s Disease Compared to Takayasu Arteritis [abstract]. Arthritis Rheumatol. 2015; 67 (suppl 10). https://acrabstracts.org/abstract/il-17-expression-by-lymphocytes-is-higher-in-behcets-disease-compared-to-takayasu-arteritis/. Accessed .« Back to 2015 ACR/ARHP Annual Meeting
ACR Meeting Abstracts - https://acrabstracts.org/abstract/il-17-expression-by-lymphocytes-is-higher-in-behcets-disease-compared-to-takayasu-arteritis/