Abstracts tagged "single cell RNA sequencing"

Abstract Number: 0053 • ACR Convergence 2021
Susceptibility Factors for Psoriatic Arthritis Single-cell RNA-Sequencing of Patients with Psoriatic Disease
Ameth Garrido¹, Rohan Machhar², Omar Cruz Correa³, Darshini Ganatra³, Sarah Crome⁴, Joan Wither³, Igor Jurisica⁵ and Dafna Gladman⁶, ¹Institute of Medical Sciences, University of Toronto, Toronto, ON, Canada, ²Krembil Research Institute, University Health Network, Toronto, ON, Canada, ³University Health Network, Toronto, ON, Canada, ⁴Ajmera Transplant Centre, Toronto General Hospital Research Institute, University Health Network, Toronto, ON, Canada, ⁵Schroeder Arthritis Institute, Krembil Research Institute, Toronto, ON, Canada, ⁶Schroeder Arthritis Institute, Krembil Research Institute, Toronto Western Hospital, Toronto, ON, Canada
Background/Purpose: Psoriatic arthritis (PsA) is an inflammatory musculoskeletal disease that affects 30% of patients with psoriasis. Thus, early diagnosis of this subgroup is crucial to…
Abstract Number: 0054 • ACR Convergence 2021
TCR Immune Profiling of Patients with Psoriatic Disease
Ameth Garrido¹, Rohan Machhar², Omar Cruz Correa³, Sarah Crome⁴, Joan Wither³, Igor Jurisica⁵ and Dafna Gladman⁶, ¹Institute of Medical Sciences, University of Toronto, Toronto, ON, Canada, ²Krembil Research Institute, University Health Network, Toronto, ON, Canada, ³University Health Network, Toronto, ON, Canada, ⁴Ajmera Transplant Centre, Toronto General Hospital Research Institute, University Health Network, Toronto, ON, Canada, ⁵Schroeder Arthritis Institute, Krembil Research Institute, Toronto, ON, Canada, ⁶Schroeder Arthritis Institute, Krembil Research Institute, Toronto Western Hospital, Toronto, ON, Canada
Background/Purpose: CD8+ and CD4+ T cell subsets have been implicated in the pathogenesis of psoriatic disease. Here, we explore the T-cell repertoire in psoriatic disease…
Abstract Number: 0460 • ACR Convergence 2021
Single-cell Profiling of B and T Cell Repertoire and Gene Expression in the RA Synovium Reveals Tissue Specific Clonal Expansion
Nida Meednu¹, Aaron Wagner², Garett Dunlap³, Fan Zhang⁴, Anna Helena Jonsson⁵, Kevin Wei⁵, Paul Utz⁶, William Robinson⁷, Holden Maecker⁷, Judith James⁸, Joel Guthridge⁸, S. Louis Bridges, Jr.⁹, Vivian Bykerk⁹, Laura Donlin⁹, Susan Goodman⁹, Edward DiCarlo⁹, Christopher Ritchlin¹⁰, Darren Tabechian², James Lederer¹¹, Ellen Gravallese¹², Mandy McGeachy¹³, Gary Firestein¹⁴, David Boyle¹⁵, Peter Gregersen¹⁶, Diane Horowitz¹⁷, Harris Perlman¹⁸, Arthur Mandelin¹⁸, Joan Bathon¹⁹, Laura Geraldino-Pardilla¹⁹, Laura Hughes²⁰, V. Michael Holers²¹, Kevin Deane²², Larry Moreland²¹, Andrew Filer²³, Costantino Pitzalis²⁴, Lindsy Forbess²⁵, Ami Ben-artzi²⁶, Karen Salomon-Escoto²⁷, Soumya Raychaudhuri⁵, Michael Brenner²⁸, Deepak Rao⁵, Andrew McDavid², Jennifer Anolik¹ and Accelerating Medicines Partership (AMP) RA/SLE Network²⁹, ¹University of Rochester Medical center, Rochester, NY, ²University of Rochester, Rochester, NY, ³Harvard University, Somerville, MA, ⁴Harvard Medical School, Boston, MA, ⁵Brigham and Women's Hospital, Boston, MA, ⁶Stanford University, Stanford, CA, ⁷Stanford University, Palo Alto, CA, ⁸Oklahoma Medical Research Foundation, Oklahoma City, OK, ⁹Hospital for Special Surgery, New York, NY, ¹⁰Division of Allergy, Immunology, and Rheumatology, School of Medicine and Dentistry, University of Rochester Medical Center, Rochester, NY, ¹¹Brigham and Women's Hospital and Harvard Medical School, Millis, MA, ¹²Brigham and Women's Hospital, Harvard Medical School, Chestnut Hill, MA, ¹³University of Pittsburgh, Pittsburgh, PA, ¹⁴University of California San Diego, San Diego, CA, ¹⁵University of California San Diego, La Jolla, CA, ¹⁶The Feinstein Institute for Medical Research, Larchmont, NY, ¹⁷Northwell Health, Jericho, NY, ¹⁸Northwestern University, Chicago, IL, ¹⁹Columbia University, New York, NY, ²⁰University of Alabama at Birmingham, Birmingham, AL, ²¹University of Colorado, Denver, CO, ²²University of Colorado Denver, Denver, CO, ²³University of Birmingham, Birmingham, United Kingdom, ²⁴Queen Mary University of London, London, United Kingdom, ²⁵Cedars-Sinai Medical Center, Los Angeles, CA, ²⁶Cedars-Sinai Medical Center, Beverly Hills, CA, ²⁷University of Massachusetts Medical School, Shrewsbury, MA, ²⁸Brigham and Women's Hospital, Harvard Medical School, Newton, MA, ²⁹Brigham and Women's Hospital, Everett, MA
Background/Purpose: B cell and T cell activation pathways in the synovium are an incompletely understood feature of rheumatoid arthritis. In this study, utilizing single cell…
Abstract Number: 0949 • ACR Convergence 2021
Single-Cell RNA Sequencing of APS Skin Reveals Endothelial Pathology and Cellular Interactions
Hui Shi¹, Allison Billi², Rachael Wasikowski¹, Claire Hoy³, Kelsey Gockman¹, Lam Tsoi¹, Johann Gudjonsson², Pei-Suen Tsou¹ and Jason Knight¹, ¹University of Michigan, Ann Arbor, MI, ²Department of Dermatology, University of Michigan, Ann Arbor, MI, ³University of Michigan, Ann Arbor
Background/Purpose: Although antiphospholipid syndrome (APS) regularly presents with discrete thrombotic events, many patients will also acquire organ damage over time secondary to occlusive neointimal formation…
Abstract Number: 1435 • ACR Convergence 2021
Identification of Distinct Fibroblast Populations in Systemic Sclerosis 3D Skin Tissues with Single Cell Omics
Noelle Kosarek¹, Heetaek Yang², Fred W. Kolling³, Tamar Abel⁴, Mengqi Huang⁵, Avi Smith⁶, Jonathan Garlick⁶, Patricia A. Pioli² and Michael L. Whitfield⁷, ¹Dartmouth Geisel School of Medicine, Hartford, VT, ²Geisel School of Medicine, Hanover, NH, ³Centers for Quantitative Biology, Hanover, ⁴Geisel School of Medicine, West Lebanon, NH, ⁵University of Pittsburgh, Pittsburgh, PA, ⁶Tufts University School of Dental Medicine, Boston, MA, ⁷Geisel School of Medicine, Lebanon, NH
Background/Purpose: Systemic sclerosis (SSc) is a rare autoimmune disease characterized by skin and internal organ fibrosis, vascular abnormalities, and autoantibody formation. Single cell genomics studies…
Abstract Number: 2806 • 2019 ACR/ARP Annual Meeting
Single Cell RNA-sequencing Reveals Distinct Macrophage Subsets in the Joint with Differing Ontogenies During Steady-state and Arthritis
Anna Montgomery ¹, Shang-Yang Chen¹, Philip Homan ¹, Gaurav Gadhvi ¹, Deborah Winter ² and Harris Perlman ², ¹Northwestern University, Chicago, ²Northwestern University Feinberg School of Medicine, Division of Rheumatology, Chicago
Background/Purpose: Macrophages in the synovial lining of the joint are critical players in the pathogenesis of rheumatoid arthritis (RA). However, their heterogeneity remains poorly characterized.Methods:…