Abstract Number: 1405 • 2017 ACR/ARHP Annual Meeting
Using Flow and Mass Cytometry to Demonstrate Robust Tissue Processing to Query Molecular Heterogeneity in Phase 1 of the Accelerating Medicines Partnership (AMP) – RA Network
Background/Purpose: Discovery and application of new therapies for rheumatoid arthritis (RA) has been hampered by multiple factors, including disease heterogeneity and the lack of well…Abstract Number: 1406 • 2017 ACR/ARHP Annual Meeting
Methods for Generating Multiple High-Dimensional Analyses of Cryopreserved Synovial Tissue Developed By the Accelerating Medicines Partnership RA/SLE Network
Background/Purpose: Detailed analyses of cells from rheumatoid arthritis (RA) synovium may identify cell phenotypes and functions that drive tissue pathology and joint damage. The AMP…Abstract Number: 1577 • 2017 ACR/ARHP Annual Meeting
Deciphering the Role of the Tissue Microenvironment in Shaping the Immune Landscape in Psoriatic Arthritis
Background/Purpose: Psoriatic arthritis (PsA) occurs in a third of patients with psoriasis (Ps) and up to 30% of patients with PsA do not have an…Abstract Number: 1736 • 2017 ACR/ARHP Annual Meeting
Deep Immunophenotyping of T-Lymphocytes with a 37-Channel Mass Cytometry (CyTOF) Panel for the Identification of Pathological Cell Functions and the Prediction of Response to Biologic Drugs in Rheumatoid Arthritis
Background/Purpose: Pathogenic immune cell types and functions have not been identified yet in rheumatoid arthritis (RA). This is explained by the impact of disease heterogeneity…Abstract Number: 1941 • 2017 ACR/ARHP Annual Meeting
Microenvironmental and Systematic Evaluation of the Immunome in Osteoarthritis
Background/Purpose: Osteoarthritis is the most common joint disease and involves whole joint, including degeneration of articular cartilage and surrounding tissues. Although widely prevalent, the exact…Abstract Number: 2420 • 2017 ACR/ARHP Annual Meeting
Mass Cytometry Analysis of CD4+ T Cells in Patients with Rheumatoid Arthritis
Background/Purpose: CD4+ T cells play a key role in the initiation and progression of RA. Past studies have identified impaired function and regulation of several…Abstract Number: 2637 • 2017 ACR/ARHP Annual Meeting
A Novel Regulatory Antigen Presenting B Cell and Memory Regulatory T Cell Subsets Are Enriched during the Quiescent Phase of Childhood Onset Systemic Lupus Erythematosus
Background/Purpose: Systemic Lupus Erythematosus (SLE) is a multi-factorial disease and the conventional oligo-dimensional investigative approach involving one or a few cell subsets at a time…Abstract Number: 133 • 2017 Pediatric Rheumatology Symposium
3-D Explant Method Facilitates the Study of Lymphocytes in Synovium and Reveals a Population of Resident Memory-Like T Cells in Rheumatoid Arthritis
Background/Purpose: Tissue resident memory T (TRM) cells survive indefinitely in barrier tissues and mediate swift immunologic memory responses at sites of microbe entry. TRM cells…Abstract Number: 3111 • 2016 ACR/ARHP Annual Meeting
Pathogenic T Cell Responses in Systemic Sclerosis Is Shaped By Novel Cytokine Axis in the Microenvironment: A Multidimensional, High Throughput Analysis
Background/Purpose: Pathogenic immune responses can be profoundly shaped by the interplay between the periphery and the microenvironment. In this work we aimed at defining the…Abstract Number: 3216 • 2016 ACR/ARHP Annual Meeting
Integrated High-Dimensional Analyses Reveal a Pathologically Expanded ‘Peripheral’ B Cell-Helper T Cell Population in Rheumatoid Arthritis
Background/Purpose: Determining the pathologic functions of T cells that infiltrate target tissues remains a central challenge in autoimmune diseases. In rheumatoid arthritis (RA), the formation…Abstract Number: 1557 • 2016 ACR/ARHP Annual Meeting
3-D Explant Method Facilitates the Study of Lymphocytes in Synovium and Reveals a Population of Resident Memory-like T Cells in Rheumatoid Arthritis
Background/Purpose: Tissue resident memory T (TRM) cells survive indefinitely in barrier tissues and mediate swift immunologic memory responses at sites of microbe entry. TRM cells…Abstract Number: 1823 • 2016 ACR/ARHP Annual Meeting
African American and European American SLE Patients with Variable Disease Activity Reveal Distinct Differences in CD4+ T Cell and Monocyte Pathways
Background/Purpose: Systemic lupus erythematosus (SLE) is an autoimmune disorder with both genetic and environmental contributions to disease etiology. Patients with different ancestral backgrounds have different…Abstract Number: 1824 • 2016 ACR/ARHP Annual Meeting
Mycophenolate Mofetil Use Associates with Unique Biologic Changes in B Cell and T Regulatory Cell Pathways in SLE Patients
Background/Purpose: The management of systemic lupus erythematosus (SLE) is complicated by a heterogeneous clinical presentation. Currently, mycophenolate mofetil (MMF) is a commonly used medication to…Abstract Number: 1840 • 2016 ACR/ARHP Annual Meeting
A Dichotomy of Regulatory Immunome Is Related to Disease Activity in Juvenile Systemic Lupus Erythematosus
Background/Purpose: The pathogenesis of SLE involves disturbances to the homeostatic balance between the immune effector and regulatory system. The conventional mono-dimensional mechanistic interrogation of one…Abstract Number: 2417 • 2016 ACR/ARHP Annual Meeting
High Dimensional Interrogation of the T Cell Immunome in Polyarticular Juvenile Idiopathic Arthritis Patients
Background/Purpose: Clinical management of polyarticular JIA with anti-TNF-alpha has been met with moderate success, with up to 50% of patients demonstrating clinically meaningful efficacy. Concerns…