Abstracts tagged "CyTOF"

Abstract Number: 2734 • 2019 ACR/ARP Annual Meeting
Signaling Lymphocytic Activation Molecule Family (SLAMF) Receptors Deregulation Is Implicated in the Altered Function of NK Cells in Systemic Lupus Erythematosus
Morgane Humbel ¹, Florence Bellanger ¹, Craig Fenwick ², Alice Horisberger ³, Camillo Ribi ³ and Denis Comte¹, ¹Service of Immunology and Allergy, Lausanne University Hospital and University of Lausanne, Epalinges, Vaud, Switzerland, ²Service of Immunology and Allergy, Lausanne University Hospital and University of Lausanne, Epalinhes, Vaud, Switzerland, ³Service of Immunology and Allergy, Lausanne University Hospital and University of Lausanne, Lausanne, Vaud, Switzerland
Background/Purpose: Systemic lupus erythematosus (SLE) is a multisystemic autoimmune disease characterized by quantitative and qualitative deficiencies of immune cells. Natural killer (NK) cells are innate…
Abstract Number: 99 • 2019 ACR/ARP Annual Meeting
Mass Cytometry Immunophenotyping of Synovial Fluid from Checkpoint Inhibitor Related Arthritis
Runci Wang¹, Karmela Kim Chan ², Amy Cunningham-Bussel ³, Laura Donlin ⁴, Gregory Vitone ², Aidan Tirpack ², Caroline Benson ², Gregory Keras ³, Anna Helena Jonsson ⁵, Michael Brenner ⁶, Anne Bass ⁷ and Deepak Rao ¹, ¹Brigham and women's hospital, Boston, MA, ²Hospital For Special Surgery, New York, ³Brigham and women's hospital, Boston, ⁴Hospital For Special Surgery, New York, NY, ⁵Brigham and Women's Hospital and Harvard Medical School, Boston, MA, ⁶Brigham and Women’s Hospital:, Boston, ⁷Hospital for Special Surgery/Weill Cornell Medicine, New York, NY
Background/Purpose: Anti-PD-1/PD-L1 checkpoint inhibitor therapies have produced remarkable results in stimulating immune responses against tumors. Checkpoint inhibitor therapy can also trigger a variety of autoimmune…
Abstract Number: 1033 • 2019 ACR/ARP Annual Meeting
Mass Cytometric Immunophenotyping Highlights a Dysregulated T cell-B Cell Axis in Patients with New-onset Lupus
Ye Cao¹, Stephen Alves ², Corine Sinnette ³, Cameron Speyer ³, Gregory Keras ¹, Yujie Qu ², Joshua Keegan ⁴, James Lederer ⁴, Deepak Rao ³ and Karen Costenbader ³, ¹Brigham and Women's Hospital, Boston, ²Merck, boston, ³Brigham and Women's Hospital, Boston, MA, ⁴BWH, Boston
Background/Purpose: The immune cell subsets most altered early in SLE disease course remain unclear. Defining abnormalities in lymphocyte populations in patients with new-onset SLE may…
Abstract Number: 135 • 2018 ACR/ARHP Annual Meeting
High Dimensional Analysis By Mass Cytometry and RNA-Sequencing Reveals Altered Frequency and Exhausted Features of CD4 T and MAIT Cells in Systemic Sclerosis
Bhairav Paleja¹, Andrea Hsiu Ling Low², Pavanish Kumar¹, Suzan Saidin¹, Ahmad Lajam², Camillus Chua³, Liyun Lai¹ and Salvatore Albani⁴, ¹Translational Immunology Institute, Singhealth/Duke-NUS Academic Medical Centre, Singapore, Singapore, ²Singapore General Hospital, Singapore, Singapore, ³Translational Immunology Institute, Singhealth/Duke-NUS Academic Medical Centre, Singapore Health Services Pte Ltd, Singapore, Singapore, ⁴Translational Immunology Institute, Singhealth/Duke-NUS Acedemic Medical Centre, Singapore, Singapore
Background/Purpose: Systemic sclerosis (SSc) is an autoimmune disease characterised by excessive fibrosis of skin and internal organs, and vascular dysfunction. Association of T and B…
Abstract Number: 1104 • 2018 ACR/ARHP Annual Meeting
Mass Cytometry Analysis Detects Dysregulated T Cell Complement Responses in Diffuse Cutaneous Systemic Sclerosis
Giuseppina Arbore¹, Shahram Kordasti², Claudia Kemper^3,4,5, Dennis Hourcade⁶, Benedetta Costantini², Leo Placais³, Lynne Mitchell⁶, Richard Ellis⁴, Christopher P. Denton⁷, David Abraham⁸ and Voon H. Ong⁸, ¹Division of Immunology, Transplantation and Infectious Diseases, San Raffaele Scientific Institute, Milano, Italy, ²Systems Cancer Immunology Lab, King's College London, London, United Kingdom, ³Laboratory of Molecular Immunology and the Immunology Center, National Institutes of Health, Washington, WA, ⁴School of Immunology and Microbial Sciences, King's College London, London, United Kingdom, ⁵Institute for Systemic Inflammation Research, University of Lübeck, Lübeck, Germany, ⁶Division of Rheumatology, Washington University School of Medicine, St Louis, WA, ⁷UCL Division of Medicine, Royal Free Campus, London, United Kingdom, ⁸Division of Medicine, University College London, London, United Kingdom
Background/Purpose: Aberrant CD4+ T cell activation is implicated in disease progression in systemic sclerosis (SSc). Activated T cells release various cytokines that may drive inflammation,…
Abstract Number: 1832 • 2018 ACR/ARHP Annual Meeting
Proteomic and Transcriptomic Profiling of Cells in Ankylosing Spondylitis Patients Identifies a Novel, Synovial-Resident CD8+ T Cell
Zoya Qaiyum¹, Eric Gracey², Yuchen Yao² and Robert D Inman³, ¹Department of Immunology, University of Toronto, Toronto, ON, Canada, ²Krembil Research Institute, Toronto Western Hospital, Toronto, ON, Canada, ³Toronto Western Hospital, University of Toronto, Spondylitis Clinic, Toronto, ON, Canada
Background/Purpose: Current data suggests that immune events in the gut may impact on joint inflammation in ankylosing spondylitis (AS) but what directs cells in the…
Abstract Number: 2029 • 2018 ACR/ARHP Annual Meeting
Extended Phenotypic Immunome Characterization (EPIC): A Web-Based Immune Reference Atlas
Joo Guan Yeo^1,2, Pan Lu¹, Thaschawee Arkachaisri^1,2, Su Li Poh¹, Fauziah Ally¹, Jing Yao Leong³, Kee Thai Yeo^1,2, Loshinidevi D/O Thana Bathi¹, Yun June Angela Tan², Seck Choon Elene Lee² and Salvatore Albani^1,4, ¹Translational Immunology Institute, Singhealth/Duke-NUS Academic Medical Centre, Singapore Health Services Pte Ltd, Singapore, Singapore, ²KK Women's and Children's Hospital, Singapore, Singapore, ³Translational Immunology Institute, Singhealth/Duke-NUS Academic Medical Centre, Singapore, Singapore, ⁴Translational Immunology Institute, Singhealth/Duke-NUS Acedemic Medical Centre, Singapore, Singapore
Background/Purpose: An atlas of the developing immune system will aid in our understanding of its normal maturation and identification of disease-associated cell subsets. The availability…
Abstract Number: 2837 • 2018 ACR/ARHP Annual Meeting
Diminished STAT-3 Phosphorylation and Associated Cell Pathways Characterize MMF-Treated Systemic Lupus Erythematosus Patients
Samantha Slight-Webb¹, Joel M. Guthridge¹, Eliza Chakravarty¹, Hua Chen¹, Rufei Lu², Krista M. Bean¹, Holden T. Maecker³, Paul J. Utz⁴ and Judith A. James⁵, ¹Arthritis and Clinical Immunology, Oklahoma Medical Research Foundation, Oklahoma City, OK, ²Oklahoma Medical Research Foundation, Oklahoma City, OK, ³Stanford University, Stanford, CA, ⁴Medicine, Stanford University School of Medicine, Stanford, CA, ⁵Arthritis & Clinical Immunology, Oklahoma Medical Research Foundation, Oklahoma City, OK
Background/Purpose: Mycophenolate mofetil (MMF) is a commonly used medication to treat major organ involvement in SLE, specifically in patients with lupus nephritis. The safety and…
Abstract Number: 2903 • 2018 ACR/ARHP Annual Meeting
Single Cell Association Testing Identifies an Expanded Th1-Skewed Cytotoxic Effector CD4+ T Cell Subset in Rheumatoid Arthritis
Chamith Fonseka¹, Deepak Rao², Nikola Teslovich³, Ilya Korsunsky⁴, Susan Hannes⁵, Kamil Slowikowski¹, Michael Gurish⁶, Laura T. Donlin⁷, James A. Lederer⁸, Michael Weinblatt⁹, Elena Massarotti⁹, Jonathan Coblyn⁹, Simon Helfgott¹⁰, Derrick J. Todd¹¹, Vivian P. Bykerk¹², Elizabeth Karlson¹³, Joerg Ermann¹⁴, Yvonne C. Lee¹⁵, Michael Brenner¹⁶ and Soumya Raychaudhuri^1,17, ¹Divisions of Genetics and Rheumatology, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, ²Human Immunology Center, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, ³Division of Genetics and Rheumatology, Department of Medicine, Harvard Medical School, Boston, MA, ⁴Division of Genetics, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, ⁵Division of Genetics, Brigham and Women’s Hospital and Harvard Medical School, Boston, MA, ⁶Division of Rheumatology, Immunology, and Allergy, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, ⁷Arthritis and Tissue Degeneration Program and the David Z. Rosensweig Genomics Research Center, Hospital for Special Surgery, New York, NY, ⁸Department of Surgery, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, ⁹Brigham and Women's Hospital, Boston, MA, ¹⁰Division of Rheumatology, Brigham and Women’s Hospital, Boston, MA, ¹¹Rheumatology, Immunology and Allergy, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, ¹²Deptartment of Rheumatology, Hospital for Special Surgery, New York, NY, ¹³Brigham and Women's Hospital and Harvard Medical School, Boston, MA, ¹⁴Division of Rheumatology, Immunology and Allergy, Brigham and Women's Hospital, Boston, MA, ¹⁵Northwestern University Feinberg School of Medicine, Chicago, IL, ¹⁶Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, ¹⁷Program in Medical and Population Genetics, Broad Institute, Boston, MA
Background/Purpose: Defining the precise CD4+ T cell subsets that are dysregulated in RA patients is critical to deciphering pathogenesis. Here we present Mixed effects modeling…
Abstract Number: 2946 • 2018 ACR/ARHP Annual Meeting
T Peripheral Helper Cells Are Expanded in the Circulation of Active SLE Patients and Correlate with CD21low B Cells
Deepak Rao¹, Alexandra Bocharnikov², Chamith Fonseka³, Joshua Keegan², Betty Diamond⁴, Jennifer Anolik⁵, Peter Nigrovic¹, Soumya Raychaudhuri^3,6, James A. Lederer⁷ and Michael Brenner⁸, ¹Rheumatology, Immunology and Allergy, Brigham and Women's Hospital, Boston, MA, ²Brigham and Women's Hospital, Boston, MA, ³Divisions of Genetics and Rheumatology, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, ⁴Autoimmune Musculoskeletal and Hematopoietic Diseases, The Feinstein Institute for Medical Research, Manhasset, NY, ⁵Medicine- Allergy, Immunology and Rheumatology, University of Rochester Medical Center, Rochester, NY, ⁶Program in Medical and Population Genetics, Broad Institute of Massachusetts Technical Institute, Harvard University, Cambridge, MA, ⁷Department of Surgery, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, ⁸Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, MA
Background/Purpose: Pathologic T cell-B cell interactions and production of autoantibodies are hallmark features of SLE. T follicular helper (Tfh) cells are generally considered the principal…
Abstract Number: 2949 • 2018 ACR/ARHP Annual Meeting
African American and European American SLE Patients with Variable Disease Activity Reveal Distinct Differences in B Cells and TLR7/8 Pathways
Samantha Slight-Webb¹, Miles C. Smith¹, Holden T. Maecker², Paul J. Utz³, Joel M. Guthridge¹ and Judith A. James⁴, ¹Arthritis and Clinical Immunology, Oklahoma Medical Research Foundation, Oklahoma City, OK, ²Stanford University, Stanford, CA, ³Medicine, Stanford University School of Medicine, Stanford, CA, ⁴Arthritis & Clinical Immunology, Oklahoma Medical Research Foundation, Oklahoma City, OK
Background/Purpose: Systemic lupus erythematosus (SLE) is an autoimmune disorder with a heterogeneous clinical presentation and periods of waxing and waning disease. Heterogeneity in SLE is…
Abstract Number: 1406 • 2017 ACR/ARHP Annual Meeting
Methods for Generating Multiple High-Dimensional Analyses of Cryopreserved Synovial Tissue Developed By the Accelerating Medicines Partnership RA/SLE Network
Deepak Rao¹, Laura T. Donlin², Kevin Wei³, Nida Meednu⁴, Jason Turner⁵, Mandy J. McGeachy⁶, Fumitaka Mizoguchi⁷, Joshua Keegan⁸, James Lederer⁹, Maria Gutierrez-Arcelus¹⁰, Kamil Slowikowski¹¹, Kaylin Muskat¹², Joshua Hillman¹², Cristina Rozo¹³, Edd Ricker¹⁴, Thomas Eisenhaure¹⁵, David Lieb¹⁵, Shuqiang Li¹⁵, Edward Browne¹⁵, Chad Nusbaum¹⁵, William H. Robinson¹⁶, Stephen Kelly¹⁷, Alessandra B. Pernis¹⁸, Lionel Ivashkiv¹⁹, Susan M. Goodman²⁰, Ellen M. Gravallese²¹, Michael Holers²², Nir Hacohen²³, Costantino Pitzalis¹⁷, Peter Gregersen²⁴, Vivian P. Bykerk²⁵, Larry W. Moreland²⁶, Gary Firestein²⁷, Soumya Raychaudhuri²⁸, Andrew Filer²⁹, David L. Boyle³⁰, Michael Brenner¹⁰ and Jennifer H. Anolik⁴, ¹Division of Rheumatology, Immunology, and Allergy, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, ²Arthritis and Tissue Degeneration Program and the David Z. Rosensweig Genomics Research Center, Hospital for Special Surgery, New York, NY, ³Division of Rheumatology, Immunology and Allergy, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, ⁴Medicine- Allergy, Immunology and Rheumatology, University of Rochester Medical Center, Rochester, NY, ⁵Institute of Inflammation and Ageing, University of Birmingham, Birmingham, United Kingdom, ⁶Medicine, University of Pittsburgh, Pittsburgh, PA, ⁷Department of Rheumatology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University (TMDU), Tokyo, Japan, ⁸Brigham and Women's Hospital, Boston, MA, ⁹Department of Surgery, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, ¹⁰Brigham and Women's Hospital and Harvard Medical School, Boston, MA, ¹¹Division of Medicine and Rheumatology, Brigham and Women's Hospital, Harvard Medical Schoo, Boston, MA, ¹²University of California, San Diego, San Diego, CA, ¹³Hospital for Special Surgery, New York, NY, ¹⁴Weill Cornell Graduate School of Medical Sciences, New York, NY, ¹⁵Broad Institute, Cambridge, MA, ¹⁶Stanford University School of Medicine, Stanford, CA, ¹⁷Centre for Experimental Medicine and Rheumatology, William Harvey Research Institute, Barts and The London, School of Medicine and Dentistry, Queen Mary University of London, London, United Kingdom, ¹⁸David Z. Rosensweig Genomics Research Center, Hospital for Special Surgery, New York, NY, ¹⁹Medicine, Hospital for Special Surgery, New York, NY, ²⁰Medicine, Hospital for Special Surgery/Weill Cornell Medicine, New York, NY, ²¹Lazare Research Bldg, University of Massachusetts Medical School, Worcester, MA, ²²Medicine, Division of Rheumatology, University of Colorado Denver, Aurora, CO, ²³Harvard Medical School, Boston, MA, ²⁴The Feinstein Institute for Medical Research, Northwell Health, Manhasset, NY, ²⁵2-005, Mt Sinai Hospital, Toronto, ON, Canada, ²⁶Rheumatology & Clinical Immunology, University of Pittsburgh, Pittsburgh, PA, ²⁷EGG, St Cloud, France, ²⁸Division of Medicine and Rheumatology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, ²⁹Institute of Inflammation and Ageing (IIA), University of Birmingham, Birmingham, United Kingdom, ³⁰University of California San Diego, La Jolla, CA
Background/Purpose: Detailed analyses of cells from rheumatoid arthritis (RA) synovium may identify cell phenotypes and functions that drive tissue pathology and joint damage. The AMP…
Abstract Number: 1577 • 2017 ACR/ARHP Annual Meeting
Deciphering the Role of the Tissue Microenvironment in Shaping the Immune Landscape in Psoriatic Arthritis
Jin Hui Sherlynn Chan¹, Ying Ying Leung², Warren Fong², Yi Wei Yeo², Bhairav Paleja¹, Liyun Lai¹, Suzan Saidin¹, Camillus Chua¹, Sharifah Nur Hazirah¹, Su Li Poh¹, Andrea Hsiu Ling Low² and Salvatore Albani^1,3, ¹SingHealth Translational Immunology and Inflammation Centre (STIIC), Duke-NUS Medical School, Singapore, Singapore, ²Singapore General Hospital, Singapore, Singapore, ³KK Women's and Children's Hospital, Singapore, Singapore
Background/Purpose: Psoriatic arthritis (PsA) occurs in a third of patients with psoriasis (Ps) and up to 30% of patients with PsA do not have an…
Abstract Number: 1736 • 2017 ACR/ARHP Annual Meeting
Deep Immunophenotyping of T-Lymphocytes with a 37-Channel Mass Cytometry (CyTOF) Panel for the Identification of Pathological Cell Functions and the Prediction of Response to Biologic Drugs in Rheumatoid Arthritis
Ben Mulhearn^1,2,3, Darren Plant², Ann W. Morgan^4,5, Anthony G. Wilson⁶, John D Isaacs^7,8, Jane Worthington⁹, Soumya Raychaudhuri^9,10,11,12, Tracy Hussell¹, Anne Barton^13,14 and Sebastien Viatte², ¹Manchester Collaborative Centre for Inflammation Research, University of Manchester, Manchester, United Kingdom, ²The University of Manchester, Arthritis Research UK Centre for Genetics and Genomics, , Centre for Musculoskeletal Research, Division of Musculoskeletal and Dermatological Sciences, Faculty of Medicine, Biology and Health, Manchester, United Kingdom, ³Kellgren Centre for Rheumatology, Central Manchester University Hospitals NHS Foundation Trust, Manchester Academic Health Science Centre, Manchester, United Kingdom, ⁴Section of Musculoskeletal Disease, NIHR-Leeds Musculoskeletal Biomedical Research Unit and Leeds Institute of Molecular Medicine, University of Leeds, Leeds, United Kingdom, ⁵Leeds Institute of Rheumatic and Musculoskeletal Medicine, University of Leeds, Leeds, United Kingdom, ⁶UCD School of Medicine and Medical Science, Conway Institute, University College Dublin, Dublin, Ireland, ⁷Institute of Cellular Medicine, Newcastle University, Newcastle-upon-Tyne, United Kingdom, ⁸National Institute for Health Research Newcastle Biomedical Research Centre, Newcastle upon Tyne Hospitals NHS Foundation Trust and Newcastle University, Newcastle, United Kingdom, ⁹Arthritis Research UK Centre for Genetics and Genomics, Centre for Musculoskeletal Research, Faculty of Biology, Medicine and Health, Manchester Academic Health Science Centre, The University of Manchester, Manchester, United Kingdom, ¹⁰Divisions of Genetics and Rheumatology, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, ¹¹Program in Medical and Population Genetics, Broad Institute of Massachusetts Technical Institute, Harvard University, Cambridge, MA, ¹²Partners Center for Personalized Genetic Medicine, Boston, MA, ¹³Arthritis Research UK Centre for Genetics and Genomics, Centre for Musculoskeletal Research, University of Manchester, Manchester, UK, Manchester, United Kingdom, ¹⁴The Kellgren Centre for Rheumatology, Central Manchester Foundation Trust, NIHR Manchester Biomedical Research Centre, Manchester, United Kingdom
Background/Purpose: Pathogenic immune cell types and functions have not been identified yet in rheumatoid arthritis (RA). This is explained by the impact of disease heterogeneity…
Abstract Number: 1941 • 2017 ACR/ARHP Annual Meeting
Microenvironmental and Systematic Evaluation of the Immunome in Osteoarthritis
Bhairav Paleja¹, Ying Ying Leung², Pavanish Kumar¹, Suzan Saidin¹, Ahmad Lajam², Liyun Lai¹ and Salvatore Albani¹, ¹SingHealth Translational Immunology and Inflammation Centre (STIIC), Duke-NUS Medical School, Singapore, Singapore, ²Singapore General Hospital, Singapore, Singapore
Background/Purpose: Osteoarthritis is the most common joint disease and involves whole joint, including degeneration of articular cartilage and surrounding tissues. Although widely prevalent, the exact…