Abstracts tagged "CyTOF"

Abstract Number: 99 • 2019 ACR/ARP Annual Meeting
Mass Cytometry Immunophenotyping of Synovial Fluid from Checkpoint Inhibitor Related Arthritis
Runci Wang¹, Karmela Kim Chan ², Amy Cunningham-Bussel ³, Laura Donlin ⁴, Gregory Vitone ², Aidan Tirpack ², Caroline Benson ², Gregory Keras ³, Anna Helena Jonsson ⁵, Michael Brenner ⁶, Anne Bass ⁷ and Deepak Rao ¹, ¹Brigham and women's hospital, Boston, MA, ²Hospital For Special Surgery, New York, ³Brigham and women's hospital, Boston, ⁴Hospital For Special Surgery, New York, NY, ⁵Brigham and Women's Hospital and Harvard Medical School, Boston, MA, ⁶Brigham and Women’s Hospital:, Boston, ⁷Hospital for Special Surgery/Weill Cornell Medicine, New York, NY
Background/Purpose: Anti-PD-1/PD-L1 checkpoint inhibitor therapies have produced remarkable results in stimulating immune responses against tumors. Checkpoint inhibitor therapy can also trigger a variety of autoimmune…
Abstract Number: 1033 • 2019 ACR/ARP Annual Meeting
Mass Cytometric Immunophenotyping Highlights a Dysregulated T cell-B Cell Axis in Patients with New-onset Lupus
Ye Cao¹, Stephen Alves ², Corine Sinnette ³, Cameron Speyer ³, Gregory Keras ¹, Yujie Qu ², Joshua Keegan ⁴, James Lederer ⁴, Deepak Rao ³ and Karen Costenbader ³, ¹Brigham and Women's Hospital, Boston, ²Merck, boston, ³Brigham and Women's Hospital, Boston, MA, ⁴BWH, Boston
Background/Purpose: The immune cell subsets most altered early in SLE disease course remain unclear. Defining abnormalities in lymphocyte populations in patients with new-onset SLE may…
Abstract Number: 2734 • 2019 ACR/ARP Annual Meeting
Signaling Lymphocytic Activation Molecule Family (SLAMF) Receptors Deregulation Is Implicated in the Altered Function of NK Cells in Systemic Lupus Erythematosus
Morgane Humbel ¹, Florence Bellanger ¹, Craig Fenwick ², Alice Horisberger ³, Camillo Ribi ³ and Denis Comte¹, ¹Service of Immunology and Allergy, Lausanne University Hospital and University of Lausanne, Epalinges, Vaud, Switzerland, ²Service of Immunology and Allergy, Lausanne University Hospital and University of Lausanne, Epalinhes, Vaud, Switzerland, ³Service of Immunology and Allergy, Lausanne University Hospital and University of Lausanne, Lausanne, Vaud, Switzerland
Background/Purpose: Systemic lupus erythematosus (SLE) is a multisystemic autoimmune disease characterized by quantitative and qualitative deficiencies of immune cells. Natural killer (NK) cells are innate…
Abstract Number: 2946 • 2018 ACR/ARHP Annual Meeting
T Peripheral Helper Cells Are Expanded in the Circulation of Active SLE Patients and Correlate with CD21low B Cells
Deepak Rao¹, Alexandra Bocharnikov², Chamith Fonseka³, Joshua Keegan², Betty Diamond⁴, Jennifer Anolik⁵, Peter Nigrovic¹, Soumya Raychaudhuri^3,6, James A. Lederer⁷ and Michael Brenner⁸, ¹Rheumatology, Immunology and Allergy, Brigham and Women's Hospital, Boston, MA, ²Brigham and Women's Hospital, Boston, MA, ³Divisions of Genetics and Rheumatology, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, ⁴Autoimmune Musculoskeletal and Hematopoietic Diseases, The Feinstein Institute for Medical Research, Manhasset, NY, ⁵Medicine- Allergy, Immunology and Rheumatology, University of Rochester Medical Center, Rochester, NY, ⁶Program in Medical and Population Genetics, Broad Institute of Massachusetts Technical Institute, Harvard University, Cambridge, MA, ⁷Department of Surgery, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, ⁸Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, MA
Background/Purpose: Pathologic T cell-B cell interactions and production of autoantibodies are hallmark features of SLE. T follicular helper (Tfh) cells are generally considered the principal…
Abstract Number: 2949 • 2018 ACR/ARHP Annual Meeting
African American and European American SLE Patients with Variable Disease Activity Reveal Distinct Differences in B Cells and TLR7/8 Pathways
Samantha Slight-Webb¹, Miles C. Smith¹, Holden T. Maecker², Paul J. Utz³, Joel M. Guthridge¹ and Judith A. James⁴, ¹Arthritis and Clinical Immunology, Oklahoma Medical Research Foundation, Oklahoma City, OK, ²Stanford University, Stanford, CA, ³Medicine, Stanford University School of Medicine, Stanford, CA, ⁴Arthritis & Clinical Immunology, Oklahoma Medical Research Foundation, Oklahoma City, OK
Background/Purpose: Systemic lupus erythematosus (SLE) is an autoimmune disorder with a heterogeneous clinical presentation and periods of waxing and waning disease. Heterogeneity in SLE is…
Abstract Number: 135 • 2018 ACR/ARHP Annual Meeting
High Dimensional Analysis By Mass Cytometry and RNA-Sequencing Reveals Altered Frequency and Exhausted Features of CD4 T and MAIT Cells in Systemic Sclerosis
Bhairav Paleja¹, Andrea Hsiu Ling Low², Pavanish Kumar¹, Suzan Saidin¹, Ahmad Lajam², Camillus Chua³, Liyun Lai¹ and Salvatore Albani⁴, ¹Translational Immunology Institute, Singhealth/Duke-NUS Academic Medical Centre, Singapore, Singapore, ²Singapore General Hospital, Singapore, Singapore, ³Translational Immunology Institute, Singhealth/Duke-NUS Academic Medical Centre, Singapore Health Services Pte Ltd, Singapore, Singapore, ⁴Translational Immunology Institute, Singhealth/Duke-NUS Acedemic Medical Centre, Singapore, Singapore
Background/Purpose: Systemic sclerosis (SSc) is an autoimmune disease characterised by excessive fibrosis of skin and internal organs, and vascular dysfunction. Association of T and B…
Abstract Number: 1104 • 2018 ACR/ARHP Annual Meeting
Mass Cytometry Analysis Detects Dysregulated T Cell Complement Responses in Diffuse Cutaneous Systemic Sclerosis
Giuseppina Arbore¹, Shahram Kordasti², Claudia Kemper^3,4,5, Dennis Hourcade⁶, Benedetta Costantini², Leo Placais³, Lynne Mitchell⁶, Richard Ellis⁴, Christopher P. Denton⁷, David Abraham⁸ and Voon H. Ong⁸, ¹Division of Immunology, Transplantation and Infectious Diseases, San Raffaele Scientific Institute, Milano, Italy, ²Systems Cancer Immunology Lab, King's College London, London, United Kingdom, ³Laboratory of Molecular Immunology and the Immunology Center, National Institutes of Health, Washington, WA, ⁴School of Immunology and Microbial Sciences, King's College London, London, United Kingdom, ⁵Institute for Systemic Inflammation Research, University of Lübeck, Lübeck, Germany, ⁶Division of Rheumatology, Washington University School of Medicine, St Louis, WA, ⁷UCL Division of Medicine, Royal Free Campus, London, United Kingdom, ⁸Division of Medicine, University College London, London, United Kingdom
Background/Purpose: Aberrant CD4+ T cell activation is implicated in disease progression in systemic sclerosis (SSc). Activated T cells release various cytokines that may drive inflammation,…
Abstract Number: 1832 • 2018 ACR/ARHP Annual Meeting
Proteomic and Transcriptomic Profiling of Cells in Ankylosing Spondylitis Patients Identifies a Novel, Synovial-Resident CD8+ T Cell
Zoya Qaiyum¹, Eric Gracey², Yuchen Yao² and Robert D Inman³, ¹Department of Immunology, University of Toronto, Toronto, ON, Canada, ²Krembil Research Institute, Toronto Western Hospital, Toronto, ON, Canada, ³Toronto Western Hospital, University of Toronto, Spondylitis Clinic, Toronto, ON, Canada
Background/Purpose: Current data suggests that immune events in the gut may impact on joint inflammation in ankylosing spondylitis (AS) but what directs cells in the…
Abstract Number: 2029 • 2018 ACR/ARHP Annual Meeting
Extended Phenotypic Immunome Characterization (EPIC): A Web-Based Immune Reference Atlas
Joo Guan Yeo^1,2, Pan Lu¹, Thaschawee Arkachaisri^1,2, Su Li Poh¹, Fauziah Ally¹, Jing Yao Leong³, Kee Thai Yeo^1,2, Loshinidevi D/O Thana Bathi¹, Yun June Angela Tan², Seck Choon Elene Lee² and Salvatore Albani^1,4, ¹Translational Immunology Institute, Singhealth/Duke-NUS Academic Medical Centre, Singapore Health Services Pte Ltd, Singapore, Singapore, ²KK Women's and Children's Hospital, Singapore, Singapore, ³Translational Immunology Institute, Singhealth/Duke-NUS Academic Medical Centre, Singapore, Singapore, ⁴Translational Immunology Institute, Singhealth/Duke-NUS Acedemic Medical Centre, Singapore, Singapore
Background/Purpose: An atlas of the developing immune system will aid in our understanding of its normal maturation and identification of disease-associated cell subsets. The availability…
Abstract Number: 2837 • 2018 ACR/ARHP Annual Meeting
Diminished STAT-3 Phosphorylation and Associated Cell Pathways Characterize MMF-Treated Systemic Lupus Erythematosus Patients
Samantha Slight-Webb¹, Joel M. Guthridge¹, Eliza Chakravarty¹, Hua Chen¹, Rufei Lu², Krista M. Bean¹, Holden T. Maecker³, Paul J. Utz⁴ and Judith A. James⁵, ¹Arthritis and Clinical Immunology, Oklahoma Medical Research Foundation, Oklahoma City, OK, ²Oklahoma Medical Research Foundation, Oklahoma City, OK, ³Stanford University, Stanford, CA, ⁴Medicine, Stanford University School of Medicine, Stanford, CA, ⁵Arthritis & Clinical Immunology, Oklahoma Medical Research Foundation, Oklahoma City, OK
Background/Purpose: Mycophenolate mofetil (MMF) is a commonly used medication to treat major organ involvement in SLE, specifically in patients with lupus nephritis. The safety and…
Abstract Number: 2903 • 2018 ACR/ARHP Annual Meeting
Single Cell Association Testing Identifies an Expanded Th1-Skewed Cytotoxic Effector CD4+ T Cell Subset in Rheumatoid Arthritis
Chamith Fonseka¹, Deepak Rao², Nikola Teslovich³, Ilya Korsunsky⁴, Susan Hannes⁵, Kamil Slowikowski¹, Michael Gurish⁶, Laura T. Donlin⁷, James A. Lederer⁸, Michael Weinblatt⁹, Elena Massarotti⁹, Jonathan Coblyn⁹, Simon Helfgott¹⁰, Derrick J. Todd¹¹, Vivian P. Bykerk¹², Elizabeth Karlson¹³, Joerg Ermann¹⁴, Yvonne C. Lee¹⁵, Michael Brenner¹⁶ and Soumya Raychaudhuri^1,17, ¹Divisions of Genetics and Rheumatology, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, ²Human Immunology Center, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, ³Division of Genetics and Rheumatology, Department of Medicine, Harvard Medical School, Boston, MA, ⁴Division of Genetics, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, ⁵Division of Genetics, Brigham and Women’s Hospital and Harvard Medical School, Boston, MA, ⁶Division of Rheumatology, Immunology, and Allergy, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, ⁷Arthritis and Tissue Degeneration Program and the David Z. Rosensweig Genomics Research Center, Hospital for Special Surgery, New York, NY, ⁸Department of Surgery, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, ⁹Brigham and Women's Hospital, Boston, MA, ¹⁰Division of Rheumatology, Brigham and Women’s Hospital, Boston, MA, ¹¹Rheumatology, Immunology and Allergy, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, ¹²Deptartment of Rheumatology, Hospital for Special Surgery, New York, NY, ¹³Brigham and Women's Hospital and Harvard Medical School, Boston, MA, ¹⁴Division of Rheumatology, Immunology and Allergy, Brigham and Women's Hospital, Boston, MA, ¹⁵Northwestern University Feinberg School of Medicine, Chicago, IL, ¹⁶Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, ¹⁷Program in Medical and Population Genetics, Broad Institute, Boston, MA
Background/Purpose: Defining the precise CD4+ T cell subsets that are dysregulated in RA patients is critical to deciphering pathogenesis. Here we present Mixed effects modeling…
Abstract Number: 297 • 2017 ACR/ARHP Annual Meeting
Multiplexed Characterization of Circulating and Joint-Derived Human Neutrophils in Inflammatory Arthritis
Ricardo Grieshaber Bouyer¹, Olha Halyabar², Anais Levescot¹, Kacie Hoyt², Lauren Henderson² and Peter Nigrovic^1,2, ¹Division of Rheumatology, Immunology and Allergy, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, ²Division of Immunology, Boston Children's Hospital, Harvard Medical School, Boston, MA
Background/Purpose: Neutrophils are innate immune cells that play a central role in the initiation of inflammatory arthritis as well as mediating tissue damage. We sought…
Abstract Number: 759 • 2017 ACR/ARHP Annual Meeting
Multi Dimensional Analysis of the Immunome in Systemic Sclerosis Reveals Functionally Related Abnormalities in MAIT and B Cell Compartments
Bhairav Paleja¹, Pavanish Kumar¹, Suzan Saidin¹, Ahmad Lajam², Camillus Chua¹, Liyun Lai¹, Andrea Hsiu Ling Low² and Salvatore Albani¹, ¹SingHealth Translational Immunology and Inflammation Centre (STIIC), Duke-NUS Medical School, Singapore, Singapore, ²Singapore General Hospital, Singapore, Singapore
Background/Purpose: Systemic sclerosis (SSc) is an autoimmune disease characterised by excessive fibrosis of skin and internal organs, and vascular dysfunction. Association of T and B…
Abstract Number: 953 • 2017 ACR/ARHP Annual Meeting
Persistence of Pathogenic CD4 Memory T Cells Revealed through Cytometry Time of Flight in Juvenile Idiopathic Arthritic Patients with Disease Resurgence upon Withdrawal of Anti-TNFA Biologics
Jing Yao Leong¹, Joo Guan Yeo², Phyllis Chen³, Liyun Lai⁴, Fauziah Ally⁵, Loshinidevi D/O Thana Bathi³, Justin Hung Tiong Tan², Thaschawee Arkachaisri², Femke van Wijk⁶, Salvatore Albani⁴, Daniel J Lovell⁷ and Gerdien Mijnheer⁸, ¹SingHealth Translational Immunology and Inflammation Centre, Singapore Health Services Pte Ltd, Singapore, Singapore, ²Rheumatology and Immunology Service, KK Women's and Children's Hospital, Singapore, Singapore, ³Singhealth Translational Immunology and Inflammation Centre (STIIC), Duke-NUS Medical School, Singapore, Singapore, ⁴SingHealth Translational Immunology and Inflammation Centre (STIIC), Duke-NUS Medical School, Singapore, Singapore, ⁵STIIC, SingHealth Translational Immunology and Inflammation Centre, Singapore, Singapore, ⁶University Medical Center Utrecht, Utrecht, Netherlands, ⁷Rheumatology, PRCSG - Cincinnati Children's Hospital Medical Center, Cinncinnati, OH, ⁸1Laboratory of Translational Immunology, Department of Paediatric Immunology, , The Netherlands, University Medical Centre Utrecht, Wilhelmina Children’s Hospital, Utrecht, Netherlands
Background/Purpose: Treatment of polyarticular JIA with anti-TNFA biologics has experienced significant success, with up to 80% of patients demonstrating clinically meaningful efficacy. Yet concerns regarding…
Abstract Number: 1405 • 2017 ACR/ARHP Annual Meeting
Using Flow and Mass Cytometry to Demonstrate Robust Tissue Processing to Query Molecular Heterogeneity in Phase 1 of the Accelerating Medicines Partnership (AMP) – RA Network
Kevin Wei¹, Deepak Rao², Fan Zhang³, Chamith Fonseka³, Kamil Slowikowski³, Joshua Keegan⁴, Laura T. Donlin⁵, Jason Turner⁶, Mandy J. McGeachy⁷, Nida Meednu⁸, David Lieb⁹, Stephen Kelly¹⁰, Susan M. Goodman¹¹, David L. Boyle¹², William H. Robinson¹³, Paul J. Utz¹⁴, Gary S. Firestein¹⁵, Harris Perlman¹⁶, Edward F. DiCarlo¹⁷, Costantino Pitzalis¹⁰, Andrew Filer¹⁸, Brendan Boyce¹⁹, Ellen M. Gravallese²⁰, Chad Nusbaum²¹, James Lederer⁴, Nir Hacohen^22,23,24, Peter Gregersen²⁵, Larry W. Moreland²⁶, Michael Holers²⁷, Vivian P. Bykerk²⁸, Soumya Raychaudhuri³, Michael Brenner²⁹ and Jennifer H. Anolik⁸, ¹Division of Rheumatology, Immunology and Allergy, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, ²Division of Rheumatology, Immunology, and Allergy, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, ³Divisions of Genetics and Rheumatology, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, ⁴Department of Surgery, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, ⁵Arthritis and Tissue Degeneration Program and the David Z. Rosensweig Genomics Research Center, Hospital for Special Surgery, New York, NY, ⁶Institute of Inflammation and Ageing, University of Birmingham, Birmingham, United Kingdom, ⁷Medicine, University of Pittsburgh, Pittsburgh, PA, ⁸Medicine- Allergy, Immunology and Rheumatology, University of Rochester Medical Center, Rochester, NY, ⁹Broad Institute, Cambridge, MA, ¹⁰Centre for Experimental Medicine and Rheumatology, William Harvey Research Institute, Barts and The London, School of Medicine and Dentistry, Queen Mary University of London, London, United Kingdom, ¹¹Medicine, Hospital for Special Surgery/Weill Cornell Medicine, New York, NY, ¹²University of California San Diego, La Jolla, CA, ¹³Stanford University School of Medicine, Stanford, CA, ¹⁴Medicine, Stanford University School of Medicine, Stanford, CA, ¹⁵Medicine, University of California San Diego, La Jolla, CA, ¹⁶Department of Medicine, Division of Rheumatology, Northwestern University Feinberg School of Medicine, Northwestern University Feinberg School of Medicine,, Chicago, IL, ¹⁷Laboratory Medicine, Hospital for Special Surgery, New York, NY, ¹⁸Institute of Inflammation and Ageing (IIA), University of Birmingham, Birmingham, United Kingdom, ¹⁹University of Rochester Medical Center, Rochester, NY, ²⁰Lazare Research Bldg, University of Massachusetts Medical School, Worcester, MA, ²¹The Broad Institute and Harvard, Cambridge, MA, ²²Harvard Medical School, Boston, MA, ²³Massachusetts General Hospital, Charlestown, MA, ²⁴Broad Institute of MIT and Harvard, Cambridge, MA, ²⁵The Feinstein Institute for Medical Research, Northwell Health, Manhasset, NY, ²⁶Rheumatology & Clinical Immunology, University of Pittsburgh, Pittsburgh, PA, ²⁷Medicine, Division of Rheumatology, University of Colorado Denver, Aurora, CO, ²⁸Divison of Rheumatology, Hospital for Special Surgery, New York, NY, ²⁹Brigham and Women's Hospital and Harvard Medical School, Boston, MA
Background/Purpose: Discovery and application of new therapies for rheumatoid arthritis (RA) has been hampered by multiple factors, including disease heterogeneity and the lack of well…

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