ACR Meeting Abstracts

ACR Meeting Abstracts

  • Meetings
    • ACR Convergence 2024
    • ACR Convergence 2023
    • 2023 ACR/ARP PRSYM
    • ACR Convergence 2022
    • ACR Convergence 2021
    • ACR Convergence 2020
    • 2020 ACR/ARP PRSYM
    • 2019 ACR/ARP Annual Meeting
    • 2018-2009 Meetings
    • Download Abstracts
  • Keyword Index
  • Advanced Search
  • Your Favorites
    • Favorites
    • Login
    • View and print all favorites
    • Clear all your favorites
  • ACR Meetings

Abstract Number: 1529

Upper Airway Gene Expression Profiling in Granulomatosis with Polyangiitis

Peter C. Grayson1, Katrina Steiling2, Paul A. Monach3, Ji Xiao4, Xiaohui Zhang2, Yuriy Alekseyev2, Stephano Monti4, Avrum Spira5 and Peter A. Merkel6, 1Section of Rheumatology & the Clinical Epidemiology Unit, Boston University School of Medicine, Vasculitis Center, Boston, MA, 2Computational Biomedicine, Boston University Medical Center, Boston, MA, 3Rheumatology, Boston University, Boston, MA, 4Computational Biomedicine, Boston University Medial Center, Boston, MA, 5Computational Biomedicine, Boston University School of Medicine, Boston, MA, 6University of Pennsylvania, Philadelphia, PA

Meeting: 2012 ACR/ARHP Annual Meeting

Keywords: RNA, Wegener's granulomatosis and vasculitis

  • Tweet
  • Click to email a link to a friend (Opens in new window) Email
  • Click to print (Opens in new window) Print
Session Information

Title: Vasculitis

Session Type: Abstract Submissions (ACR)

Background/Purpose: Nasal disease occurs in the majority of patients with granulomatosis with polyangiitis (Wegener’s, GPA) and is often a presenting symptom of the disease. The objectives of this study were to use gene expression profiling techniques to gain insight into the biology of upper airway disease in GPA and explore the potential utility of genome-wide gene expression signatures as measures of nasal disease activity.

Methods: Nasal brushings of the inferior turbinate were obtained from 32 subjects with GPA (n=10 active nasal disease, n=13 prior nasal disease, n=9 never nasal disease) and 35 comparator subjects with and without inflammatory nasal disease (n=12 healthy, n=15 sarcoidosis, n=8 allergic rhinitis). RNA extracted from the brushings was processed and hybridized to Affymetrix Human Gene 1.0 ST Arrays. Gene expression changes associated with nasal disease activity were identified with a linear mixed effects model controlling for microarray batch, use of prednisone, and use of other immunosuppressant medication. Significant differences were defined at a threshold of false discovery rate (FDR) <0.1 and fold change > 1.5. Functional enrichment of biologic pathways among the gene expression profiles associated with nasal disease activity was determined using Gene Set Enrichment Analysis (GSEA) (FDRGSEA<0.25). The relationship of nasal gene expression profiles to peripheral blood mononuclear and neutrophil gene expression levels associated with GPA (Cheadle et al A&R 2010) was determined using GSEA.

Results: The expression levels of 452 genes were associated with active nasal disease, 309 with prior nasal disease, and 20 never nasal disease in subjects with GPA. GSEA revealed enrichment of several biologic pathways among genes associated with nasal disease activity. The 20 most significantly enriched pathways among subjects with active nasal disease were also significantly enriched among subjects with prior nasal disease and included pathways related to immune response (eg  HSA04679 Leukocyte Transendothelial Migration) and thrombosis (eg HSA04610 Complement And Coagulation Cascade). There was no overlap between biologic pathways enriched in subjects with never nasal disease, and pathways enriched among subjects with active or prior nasal disease. Peripheral blood neutrophil and mononuclear gene expression levels associated with GPA were similarly altered in the nasal gene expression profiles of subjects with active or prior nasal disease, but were not significantly enriched in subjects with never nasal disease.

Conclusion: Nasal gene expression profiles are associated with nasal disease activity in subjects with GPA. Pathways analysis suggests that the biologic functions of genes altered in subjects with active nasal disease are similar to subjects with prior nasal disease, and distinct from subjects with never nasal disease. Upper airway gene expression profiles in subjects with active and prior nasal disease were similar to patterns of gene expression changes derived from fractionated peripheral blood in GPA, suggesting that nasal gene expression profiles in GPA may reflect systemic disease activity.


Disclosure:

P. C. Grayson,
None;

K. Steiling,
None;

P. A. Monach,
None;

J. Xiao,
None;

X. Zhang,
None;

Y. Alekseyev,
None;

S. Monti,
None;

A. Spira,
None;

P. A. Merkel,
None.

  • Tweet
  • Click to email a link to a friend (Opens in new window) Email
  • Click to print (Opens in new window) Print

« Back to 2012 ACR/ARHP Annual Meeting

ACR Meeting Abstracts - https://acrabstracts.org/abstract/upper-airway-gene-expression-profiling-in-granulomatosis-with-polyangiitis/

Advanced Search

Your Favorites

You can save and print a list of your favorite abstracts during your browser session by clicking the “Favorite” button at the bottom of any abstract. View your favorites »

All abstracts accepted to ACR Convergence are under media embargo once the ACR has notified presenters of their abstract’s acceptance. They may be presented at other meetings or published as manuscripts after this time but should not be discussed in non-scholarly venues or outlets. The following embargo policies are strictly enforced by the ACR.

Accepted abstracts are made available to the public online in advance of the meeting and are published in a special online supplement of our scientific journal, Arthritis & Rheumatology. Information contained in those abstracts may not be released until the abstracts appear online. In an exception to the media embargo, academic institutions, private organizations, and companies with products whose value may be influenced by information contained in an abstract may issue a press release to coincide with the availability of an ACR abstract on the ACR website. However, the ACR continues to require that information that goes beyond that contained in the abstract (e.g., discussion of the abstract done as part of editorial news coverage) is under media embargo until 10:00 AM ET on November 14, 2024. Journalists with access to embargoed information cannot release articles or editorial news coverage before this time. Editorial news coverage is considered original articles/videos developed by employed journalists to report facts, commentary, and subject matter expert quotes in a narrative form using a variety of sources (e.g., research, announcements, press releases, events, etc.).

Violation of this policy may result in the abstract being withdrawn from the meeting and other measures deemed appropriate. Authors are responsible for notifying colleagues, institutions, communications firms, and all other stakeholders related to the development or promotion of the abstract about this policy. If you have questions about the ACR abstract embargo policy, please contact ACR abstracts staff at [email protected].

Wiley

  • Online Journal
  • Privacy Policy
  • Permissions Policies
  • Cookie Preferences

© Copyright 2025 American College of Rheumatology