Session Type: ACR Poster Session B
Session Time: 9:00AM-11:00AM
Background/Purpose: Circulating multipotential stromal cells (MSCs) also termed mesenchymal stem cells have previously been implicated in fibroblast mediated polyarticular joint destruction in rheumatoid arthritis (RA). We hypothesized that skeletal trauma (a biophysical process) rather than a biological basis related to RA may account for any MSC circulation.
Methods: Deep (femoral) and matched peripheral (antecubital) vein blood was collected from 36 patients undergoing lower limb orthopaedic procedures. Peripheral blood was also taken from 15 early and 11 established RA patients as well as 12 healthy controls. Colony-forming unit-fibroblast (CFU-F) assays and cytometric phenotyping of cells were performed. Molecular characterisation of genes related to MSC function was undertaken in comparison to MSCs from iliac crest and femoral marrows, bone, periosteum, adipose tissue and dermal fibroblasts.
Results: 17/36 femoral vein samples contained CFU-Fs, but only 7/74 peripheral vein samples almost exclusively from the orthopaedic cases with only a single peripheral blood colony from one established RA patient. The MSC nature of CFU-Fs was confirmed by expansion and phenotype: CD105/CD73/CD90 positivity and CD19/CD31/CD33/CD34/CD45/CD61 negativity. Their molecular profiles were typical of MSCs with 39/80 genes showing similarity across multiple MSC tissue controls; including osteogenesis-related ALPL, COL1A1, SPARC, adipogenic LPL, chondrogenic COL2A1, COL10A1, SOX9 and immature POU5F, NANOG, but not fibroblasts.
To cite this abstract in AMA style:Churchman S, Boxall S, Jones E, Emery P, Giannoudis P, McGonagle D. Transient Circulatory Existence of Multipotential Stromal Cells Is Unlikely to Contribute to the Pathogenesis of Rheumatoid Arthritis [abstract]. Arthritis Rheumatol. 2016; 68 (suppl 10). https://acrabstracts.org/abstract/transient-circulatory-existence-of-multipotential-stromal-cells-is-unlikely-to-contribute-to-the-pathogenesis-of-rheumatoid-arthritis/. Accessed July 5, 2020.
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ACR Meeting Abstracts - https://acrabstracts.org/abstract/transient-circulatory-existence-of-multipotential-stromal-cells-is-unlikely-to-contribute-to-the-pathogenesis-of-rheumatoid-arthritis/