Date: Monday, October 22, 2018
Session Title: Systemic Lupus Erythematosus – Etiology and Pathogenesis Poster II
Session Type: ACR Poster Session B
Session Time: 9:00AM-11:00AM
Background/Purpose: This study, developed within the IMI-JU project PRECISESADS framework, aimed to determine the enrichment on CD14+ and CD16+ monocyte subpopulations in SLE, APS and APS+SLE patients, and to investigate their profiles and role in the pathogenesis of these diseases.
Methods: The frequencies of monocyte subpopulations in the peripheral blood of 146 healthy donors and 140 SLE patients included in the PRECISESADS study were determined by flow cytometry. Two additional cohorts of 21 APS+SLE and 19 APS patients were also evaluated. Clinical features, proinflammatory circulating mediators, as well as the prothrombotic/proinflammatory/pro-oxidative profiles of monocytes subsets -at both gene and protein levels- were analyzed.
Results: The frequencies of CD14+CD16++ (non-classical) monocytes were reduced in SLE patients, while CD14+CD16+ (intermediate) monocytes were increased. The reduced frequencies of CD16+ monocytes were negatively associated with the positivity for anti-dsDNA autoantibodies, as well as with renal involvement -demonstrated by biopsy-confirmed nephritis, proteinuria, and presence of macrophages in urine samples-, all of which might reflect a recruitment process of this subset in renal tissues. Correlation studies indicated a link between the reduced frequency of non-classical monocytes and increased levels of circulating inflammatory mediators. Conversely, in SLE patients with augmented cardiovascular risk, increased frequencies of CD14+CD16+ monocytes were observed. These results prompted us to evaluate the proportion and profile of this inflammatory subtype in parallel cohorts of SLE+APS and APS patients, on which thrombosis is the main clinical disorder.
SLE+APS patients showed enrichment in both CD16+ subsets of monocytes, associated with the positivity for anti-dsDNA antibodies and the presence of atheroma plaques. Correlations among the frequency of those monocyte subsets and circulating inflammatory mediators were also demonstrated. Moreover, these subsets displayed an increased inflammatory and prothrombotic profile when compared with classical CD14+ monocytes.
In APS patients we also saw enrichment of the CD16+ inflammatory subsets, associated
Conclusion: Circulating CD16+ monocytes might constitute a subpopulation of proinflammatory cells whose frequency and molecular profiles might identify APS, APS+SLE and SLE patients suffering thrombosis, atherosclerosis and organ involvement.
Supported by the EU/EFPIA –IMI-JU PRECISESADS (n° 115565), FIS PI15/1333, Co-funded with FEDER.
To cite this abstract in AMA style:Lopez-Pedrera C, Aguirre Zamorano MÁ, Barbarroja N, Ruiz-Limon P, Abalos-Aguilera MC, Jiménez-Gómez Y, Arias de la Rosa I, Segui P, Ortega-Castro R, Collantes Estevez E, Escudero-Contreras A, Le Lann L, Jamin C, Marañón C, Alarcón-Riquelme M, Pers JO, Perez-Sanchez C. The Frequencies and Molecular Profiles of CD16+ Monocyte Subsets in Patients with Systemic Lupus Erythematosus, Primary Antiphospholipid Syndrome, and Antiphospholipid Syndrome with Lupus, Identify Specific Clinical Features of These Diseases [abstract]. Arthritis Rheumatol. 2018; 70 (suppl 9). https://acrabstracts.org/abstract/the-frequencies-and-molecular-profiles-of-cd16-monocyte-subsets-in-patients-with-systemic-lupus-erythematosus-primary-antiphospholipid-syndrome-and-antiphospholipid-syndrome-with-lupus-identify-sp/. Accessed March 23, 2023.
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