ACR Meeting Abstracts

ACR Meeting Abstracts

Abstract Number: 1100

The Frequencies and Molecular Profiles of CD16+ Monocyte Subsets in Patients with Systemic Lupus Erythematosus, Primary Antiphospholipid Syndrome, and Antiphospholipid Syndrome with Lupus, Identify Specific Clinical Features of These Diseases

Chary Lopez-Pedrera1, Maria Ángeles Aguirre Zamorano1, Nuria Barbarroja2, Patricia Ruiz-Limon3, Maria Carmen Abalos-Aguilera4, Yolanda Jiménez-Gómez2, Ivan Arias de la Rosa4, Pedro Segui5, Rafaela Ortega-Castro2, Eduardo Collantes Estevez1, Alejandro Escudero-Contreras4, Lucas Le Lann6, Christophe Jamin6, Concepcion Marañón7, Marta Alarcón-Riquelme8, Jacques-Olivier Pers6 and Carlos Perez-Sanchez2, 1IMIBIC/Reina Sofia Hospital/University of Cordoba, Cordoba, Spain, 2Rheumatology service, IMIBIC/Reina Sofia Hospital/University of Cordoba, Cordoba, Spain, 3Research Group of Endocrine Diseases, Research Laboratory. Biomedical Research Institute of Malaga (IBIMA).Virgen de la Victoria Universitary Hospital, Malaga, Spain., Málaga, MA, Spain, 4Rheumatology Service, IMIBIC/Reina Sofia Hospital/University of Cordoba, Cordoba, Spain, 5Radiology, IMIBIC/Reina Sofia Hospital/University of Cordoba, Cordoba, Spain, 6U1227, Université de Brest, Inserm, Labex IGO, CHU de Brest, Brest, France, 7GENYO, Centre for Genomics and Oncological Research Pfizer, University of Granada, Andalusian Regional Government, Granada, Spain, 8Medical Genomics, Center for Genomics and Oncological Research (GENYO), Granada, Spain

Meeting: 2018 ACR/ARHP Annual Meeting

Keywords: , , ,

Session Information

Date: Monday, October 22, 2018

Title: Systemic Lupus Erythematosus – Etiology and Pathogenesis Poster II

Session Type: ACR Poster Session B

Session Time: 9:00AM-11:00AM

Background/Purpose: This study, developed within the IMI-JU project PRECISESADS framework, aimed to determine the enrichment on CD14+ and CD16+ monocyte subpopulations in SLE, APS and APS+SLE patients, and to investigate their profiles and role in the pathogenesis of these diseases.

Methods: The frequencies of monocyte subpopulations in the peripheral blood of 146 healthy donors and 140 SLE patients included in the PRECISESADS study were determined by flow cytometry. Two additional cohorts of 21 APS+SLE and 19 APS patients were also evaluated. Clinical features, proinflammatory circulating mediators, as well as the prothrombotic/proinflammatory/pro-oxidative profiles of monocytes subsets -at both gene and protein levels- were analyzed.

Results: The frequencies of CD14+CD16++ (non-classical) monocytes were reduced in SLE patients, while CD14+CD16+ (intermediate) monocytes were increased. The reduced frequencies of CD16+ monocytes were negatively associated with the positivity for anti-dsDNA autoantibodies, as well as with renal involvement -demonstrated by biopsy-confirmed nephritis, proteinuria, and presence of macrophages in urine samples-, all of which might reflect a recruitment process of this subset in renal tissues. Correlation studies indicated a link between the reduced frequency of non-classical monocytes and increased levels of circulating inflammatory mediators. Conversely, in SLE patients with augmented cardiovascular risk, increased frequencies of CD14+CD16+ monocytes were observed. These results prompted us to evaluate the proportion and profile of this inflammatory subtype in parallel cohorts of SLE+APS and APS patients, on which thrombosis is the main clinical disorder.

SLE+APS patients showed enrichment in both CD16+ subsets of monocytes, associated with the positivity for anti-dsDNA antibodies and the presence of atheroma plaques. Correlations among the frequency of those monocyte subsets and circulating inflammatory mediators were also demonstrated. Moreover, these subsets displayed an increased inflammatory and prothrombotic profile when compared with classical CD14+ monocytes.

In APS patients we also saw enrichment of the CD16+ inflammatory subsets, associated to recurrent thrombotic events and pathologic carotid intima-media thickness. The scores of various markers related to autoimmunity, oxidative stress and prothrombotic molecules correlated with the proportions of CD16+ monocytes, which showed an activated profile similar to that observed in SLE+APS patients.

Conclusion: Circulating CD16+ monocytes might constitute a subpopulation of proinflammatory cells whose frequency and molecular profiles might identify APS, APS+SLE and SLE patients suffering thrombosis, atherosclerosis and organ involvement.

Supported by the EU/EFPIA –IMI-JU PRECISESADS (n° 115565), FIS PI15/1333, Co-funded with FEDER.

Disclosure: C. Lopez-Pedrera, None; M. Á. Aguirre Zamorano, None; N. Barbarroja, None; P. Ruiz-Limon, None; M. C. Abalos-Aguilera, None; Y. Jiménez-Gómez, None; I. Arias de la Rosa, None; P. Segui, None; R. Ortega-Castro, None; E. Collantes Estevez, None; A. Escudero-Contreras, None; L. Le Lann, Servier, 2; C. Jamin, Servier, 2; C. Marañón, None; M. Alarcón-Riquelme, Sanofi, Bayer, UCB, Eli Lilly and Servier, 2; J. O. Pers, None; C. Perez-Sanchez, None.

To cite this abstract in AMA style:

Lopez-Pedrera C, Aguirre Zamorano MÁ, Barbarroja N, Ruiz-Limon P, Abalos-Aguilera MC, Jiménez-Gómez Y, Arias de la Rosa I, Segui P, Ortega-Castro R, Collantes Estevez E, Escudero-Contreras A, Le Lann L, Jamin C, Marañón C, Alarcón-Riquelme M, Pers JO, Perez-Sanchez C. The Frequencies and Molecular Profiles of CD16+ Monocyte Subsets in Patients with Systemic Lupus Erythematosus, Primary Antiphospholipid Syndrome, and Antiphospholipid Syndrome with Lupus, Identify Specific Clinical Features of These Diseases [abstract]. Arthritis Rheumatol. 2018; 70 (suppl 9). https://acrabstracts.org/abstract/the-frequencies-and-molecular-profiles-of-cd16-monocyte-subsets-in-patients-with-systemic-lupus-erythematosus-primary-antiphospholipid-syndrome-and-antiphospholipid-syndrome-with-lupus-identify-sp/. Accessed .

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