Session Title: Rheumatoid Arthritis - Clinical Aspects Poster Session III
Session Type: ACR Poster Session C
Session Time: 9:00AM-11:00AM
Background/Purpose: The purpose of this study is to explore new biomarkers for early diagnosis of rheumatoid arthritis (RA). For better discrimination power, we especially investigated the samples of early RA (eRA) patients whose onset of RA were less than 1 year.
Methods: We examined the serum cytokines and the gene expression profiles of peripheral mononuclear cells from early rheumatoid arthritis (eRA). eRA samples were compared with advanced RA (aRA, disease duration more than 1 year) and osteoarthritis. Serum obtained from 44 patients with eRA were evaluated for cytokine panels, including IFN-r, IL-12, IL-2, IL-4, IL-10, IL-17 and IL-6. Gene expression profiles of eRA (n=11), using microarray analysis, were compared with OA (n=8) and aRA (n=7). Biomarker candidates were positively selected by pathway analysis using NCBI database about Th17 cell differentiation genes.
Results: Mean age of eRA and aRA were 51.9 and 58.1 respectively. CRP level was higher in aRA than eRA (3.27 vs 1.19 mg/dL, P<0.02). Th17 cell-related cytokines like IL-6 and IL-17 were prominent than other cytokines in eRA patients. IL-17 concentration was statistically higher in eRA than aRA. Positive percentage of IL-6 was more than 50% in eRA patients. IL-6 were well correlated with ESR (r=0.3, P=0.006) and CRP (r=0.6, P<0.001). Interestingly, IL-17 levels were correlated with disease activity DAS28 (r=0.45, P=0.003). 255 genes were upregulated in eRA (|F.C|>1.3, P<0.05). 10 genes were selected from 255 genes by association analysis with Th17 differentiation as followings, TMEM140 (transmembrane protein 140), NUAK2 (NUAK family, SNF1-like kinase 2), SULT1A3 (sulfotransferase family, cytosolic, 1A, phenol-preferring, member 3), HIST1H4H (histone cluster 1, H4h), NAPSB (napsin B aspartic peptidase pseudogene), FEN1 (flap structure-specific endonuclease 1), PROS1 (protein S alpha), RPS6KA2 (ribosomal protein S6 kinase, 90kDa, polypeptide 2), CDKN3 (cyclin-dependent kinase inhibitor 3), JAM3 (junctional adhesion molecule 3).
Conclusion: Th17-related cytokines were dominant at early stage of RA and associated with disease activity. Th17-related candidate genes could give a new insight as biomarkers for early diagnosis and therapeutic target for eRA.
To cite this abstract in AMA style:Kim Y, Lee K, Kim HR, Lee SH, Ju JH, KIM HY. Th17 Cell Related Cytokines and Microarray Expression Profiles in Patients with Early Rheumatoid Arthritis [abstract]. Arthritis Rheumatol. 2015; 67 (suppl 10). https://acrabstracts.org/abstract/th17-cell-related-cytokines-and-microarray-expression-profiles-in-patients-with-early-rheumatoid-arthritis/. Accessed July 9, 2020.
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