Targeted 1H NMR Based Metabolomics Analysis Revealed Significantly Higher Synovial Phe/Tyr Ratio in Reactive Arthritis and Undifferentiated Spondyloarthropathy

Hafis Muhammed¹, Dinesh Kumar ², Durgesh Dubey ², Sandeep Kumar ³, Smriti Chaurasia ³, Anupam Guleria ², Sanjukta Majumder ³, Rajeev Singh ³, Vikas Agarwal ¹ and Ramnath Misra ⁴, ¹SGPGI, Lucknow, Uttar Pradesh, India, ²Centre for Biomedical Research, Sanjay Gandhi Post Graduate Institute of Medical Science, Lucknow, Uttar Pradesh, India, ³Department of Clinical Immunology and Rheumatology, Sanjay Gandhi Post Graduate Institute of Medical Science, Lucknow, Uttar Pradesh, India, ⁴Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow, Lucknow, Uttar Pradesh, India

Meeting: 2019 ACR/ARP Annual Meeting

Keywords: reactive arthritis and metabolomics, Spondylarthritis

Session Information

Date: Sunday, November 10, 2019

Title: Spondyloarthritis Including Psoriatic Arthritis – Clinical Poster I: Axial Spondyloarthritis, Clinical Features

Session Type: Poster Session (Sunday)

Session Time: 9:00AM-11:00AM

Background/Purpose: Elevated phenylalanine-to-tyrosine ratio (Phe/Tyr) is a marker of oxidative stress. We hypothesized that the synovial Phe/Tyr in reactive arthritis is higher than Rheumatoid arthritis(RA) and osteoarthritis(OA). We conducted this study with the objective to compare the synovial Phe/Tyr between ReA/uSpA and RA and OA by NMR spectroscopy

Methods: Paired synovial fluid and serum of 30 patients with ReA/uSpA were collected and analysed using a 1D ¹H CPMG NMR spectra recorded on 800 MHz NMR spectrometer equipped with a TCI Cryoprobe (at 300 k). Phenylalanine and tyrosine were quantified. Synovial fluid from 25 patients with RA fulfilling ACR classification criteria and 21 patients with OA were taken as inflammatory and non-inflammatory controls.

Results: Synovial Phe/Tyr ratio was significantly higher in ReA/uSpA compared to RA and OA. Synovial Phe/Tyr ratios were comparable in RA and OA patients. Compared to serum, the Phe/Tyr was significantly higher in the SF in ReA/uSpA. The Phe/Tyr was also found to be positively correlated between serum and SF samples with regression coefficient (r²) of 0.287.

Conclusion: NMR based metabolomics study demonstrates that the synovial Phe/Tyr are specifically elevated in ReA/uSpA.

Synovial Phe/Tyr ratio was significantly higher in ReA/uSpA compared to RA and OA

Disclosure: H. Muhammed, None; D. Kumar, None; D. Dubey, None; S. Kumar, None; S. Chaurasia, None; A. Guleria, None; S. Majumder, None; R. Singh, None; V. Agarwal, None; R. Misra, None.

To cite this abstract in AMA style:

Muhammed H, Kumar D, Dubey D, Kumar S, Chaurasia S, Guleria A, Majumder S, Singh R, Agarwal V, Misra R. Targeted 1H NMR Based Metabolomics Analysis Revealed Significantly Higher Synovial Phe/Tyr Ratio in Reactive Arthritis and Undifferentiated Spondyloarthropathy [abstract]. Arthritis Rheumatol. 2019; 71 (suppl 10). https://acrabstracts.org/abstract/targeted-1h-nmr-based-metabolomics-analysis-revealed-significantly-higher-synovial-phe-tyr-ratio-in-reactive-arthritis-and-undifferentiated-spondyloarthropathy/. Accessed .

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