Session Title: Rheumatoid Arthritis - Human Etiology and Pathogenesis
Session Type: Abstract Submissions (ACR)
Previous studies have shown increased expression of stromal markers in synovial tissue (ST) of patients with established rheumatoid arthritis (RA). Here, the expression of tissue expressed stromal markers in early arthritis in relationship to diagnosis and outcome was studied.
ST from 67 patients included in two different early arthritis cohorts (Birmingham and Amsterdam) and seven non-inflammatory controls was analysed using immunofluorescence to detect the stromal markers CD55, CD248, fibroblast activation protein (FAP) and podoplanin. Diagnostic classification (gout, psoriatic arthritis, unclassified arthritis (UA), parvovirus associated arthritis, reactive arthritis and RA) and outcome (resolving or persistent) was determined at baseline and after follow-up. The relationship between the expression of the stromal markers and diagnosis and outcome was determined.
We observed expression of all stromal markers in ST of early arthritis patients, independent of diagnosis or prognostic outcome. Expression of FAP and podoplanin was significantly higher in patients with early RA compared to non-inflammatory controls (p=0.003 and p=0.021, respectively). Significantly greater expression of FAP was found in anti-citrullinated peptide antibody (ACPA)-negative RA patients and in patients with UA fulfilling classification criteria for RA after follow-up compared to patients with resolving disease and patients with persistent disease who did not fulfil classification criteria for RA after follow-up (p=0.030 and p=0.020, respectively for ACPA-negative RA patients and p=0.045 and p=0.024, respectively for UA patients fulfilling classification criteria for RA after follow-up).
The stromal cell markers CD55, CD248, FAP and podoplanin, are expressed in the synovium in the earliest stage of arthritis. Expression of FAP is higher in early unclassified arthritis patients who fulfil classification criteria for RA over time and in ACPA-negative RA compared to resolving or non-RA arthritides. These results suggest that significant fibroblast activation occurs in RA in the early window of disease.
I. Y. K. Choi,
O. N. Karpus,
J. D. Turner,
D. L. Hardie,
M. J. H. de Hair,
K. I. Maijer,
P. P. Tak,
D. M. Gerlag,
« Back to 2014 ACR/ARHP Annual Meeting
ACR Meeting Abstracts - https://acrabstracts.org/abstract/stromal-cell-markers-are-differentially-expressed-in-the-synovial-tissue-of-patients-with-early-arthritis/