Session Information
Session Type: ACR Poster Session C
Session Time: 9:00AM-11:00AM
Background/Purpose:
14-3-3 proteins are chaperonins found in all eukaryotic cells. There are multiple isoforms which are thought to be involved in intracellular signaling and transcription regulation. Among their targets are phosphatases, kinases and transmembrane receptors. There are seven known isoforms in mammals. The η (Eta) isoform has been implicated as having diagnostic potential in inflammatory arthritis. In this study we investigated the utility of measuring 14-3-3η when evaluating inflammatory arthritis.
Methods:
Measurements of 14-3-3η were obtained during evaluation of joint pain in patients presenting between July 2013 and April of 2015. Rheumatoid factor (RF) and anti-cyclic citrullinated peptide antibodies (anti-CCP Ab) were measured. Joint imaging was evaluated for erosive changes and use of biologics was recorded. A chart review was later conducted to evaluate the utility of standard measures versus 14-3-3η.
Results:
214 patients were evaluated. 49 had a diagnosis of rheumatoid arthritis (RA), 17 had psoriatic arthritis (PsA). 43 patients had connective tissue disease (CTD), 8 had undifferentiated inflammatory arthritis, and 97 had other rheumatological disorders.
Of the 49 RA patients, 37 were RF positive, 36 were anti-CCP Ab positive, and 25 were 14-3-3η positive. Of the 25 who were 14-3-3η positive, 22 were both anti-CCP Ab and RF positive, 2 were RF positive but anti-CCP Ab neg, and 1 was negative for both RF and anti-CCP Ab. Of the 24 who were 14-3-3η negative, 12 were both RF and anti-CCP Ab positive, 2 were only anti-CCP Ab positive, 1 was only RF positive, and 9 were seronegative. 20 patients in total had erosive disease of which 13 were positive for 14-3-3η, 10 were positive for all three markers, 1 was positive for just RF, and 1 was negative for both RF and anti-CCP Ab. Of the RA patients with erosive changes on biologic medication, 5 were 14-3-3η positive and 8 were 14-3-3η negative.
All 17 patients with PsA were seronegative with one exception, who was 14-3-3η positive. 6 PsA patients had joint erosions and they were seronegative. Of the 43 patients with CTD, 6 were 14-3-3η positive. 2 patients had enteropathic arthritis and were seronegative.
|
14.3.3η alone |
RF and CCP |
Triple Positive |
14.3.3η and RF |
14.3.3η and CCP |
Any seropositivity |
|
|
RA sensitivity |
0.51 |
0.69 |
0.45 |
0.49 |
0.45 |
0.82 |
|
RA specificity |
0.90 |
0.97 |
0.98 |
0.94 |
0.98 |
0.79 |
|
RA PPV |
0.49 |
0.50 |
0.50 |
0.50 |
0.50 |
0.49 |
|
RA NPV |
0.88 |
0.92 |
0.86 |
0.87 |
0.86 |
0.95 |
Conclusion:
The 14-3-3η protein can be detected in inflammatory arthritis, with the highest percentage in RA patients. However, there was no correlation between 14-3-3η titers with RF or anti-CCP Ab. No significant association was found with PsA patients in our small sample. There was also no correlation with biologic use. One 14-3-3η positive patient who was negative for RF and anti-CCP Ab had erosive disease.
To cite this abstract in AMA style:
Feller L, Tuttle P IV, Dalrymple AM, Bathula S, Temprano K, Syed RH, Bandlamudi R, Coulson K, Pepmueller P, Moore T. Single Academic Center Experience with 14-3-3 Eta in the Evaluation of Inflammatory Arthritis [abstract]. Arthritis Rheumatol. 2015; 67 (suppl 10). https://acrabstracts.org/abstract/single-academic-center-experience-with-14-3-3-eta-in-the-evaluation-of-inflammatory-arthritis/. Accessed .« Back to 2015 ACR/ARHP Annual Meeting
ACR Meeting Abstracts - https://acrabstracts.org/abstract/single-academic-center-experience-with-14-3-3-eta-in-the-evaluation-of-inflammatory-arthritis/