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Abstract Number: 2586

Results from the Biopsy Driven Ebio Study: Entheseal Tissue Signature in Response to IL-17 Blockade in Psoriatic Arthritis

Maria Gabriella Raimondo1, Hashem Mohammadian2, Stefano Alivernini3, Simon Rauber1, Filippo Fagni4, Giulia Corte5, Koray Tascilar2, Maria Antonietta D´Agostino6, Georg Schett7, Arnd Kleyer8 and Andreas Ramming1, 1Department of Medicine 3 - Rheumatology and Immunology, Friedrich-Alexander-Universität (FAU) Erlangen-Nürnberg and Uniklinikum Erlangen, Erlangen, Bayern, Germany, 2Department of Internal Medicine 3, Rheumatology & Immunology, Friedrich-Alexander-University (FAU) Erlangen-Nurnberg and Universitätsklinikum Erlangen, Erlangen, Germany, Erlangen, Germany, 3Division of Rheumatology - Fondazione Policlinico Universitario A. Gemelli IRCCS - Università Cattolica del Sacro Cuore, Rome, Italy, Rome, Italy, 4Department of Internal Medicine 3 - Rheumatology and Immunology, Friedrich-Alexander University (FAU) Erlangen-Nürnberg and Universitätsklinikum Erlangen, Erlangen, Germany, Deutsches Zentrum für Immuntherapie (DZI), Friedrich Alexander University Erlangen-Nuremberg and Universitätsklinikum Erlangen, Erlangen, Germany, Erlangen, Germany, 5Department of Internal Medicine 3 - Rheumatology and Immunology, Friedrich-Alexander University (FAU) Erlangen-Nürnberg and Universitätsklinikum Erlangen, Erlangen, Germany, Deutsches Zentrum für Immuntherapie (DZI), Friedrich Alexander University Erlangen-Nuremberg and Universitätsklinikum Erlangen, Erlangen, Germany, Erlangen, Bayern, Germany, 6Catholic University of Sacred Heart, Rome, Italy, Rome, Italy, 7Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, Germany, 8Charité - Universittsmedizin Berlin, Erlangen, Germany

Meeting: ACR Convergence 2024

Keywords: Bioinformatics, Biologicals, Imaging, Psoriatic arthritis

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Session Information

Date: Monday, November 18, 2024

Title: Abstracts: SpA Including PsA – Treatment II

Session Type: Abstract Session

Session Time: 1:00PM-2:30PM

Background/Purpose: Enthesitis is considered to be an early event in psoriatic arthritis (PsA) course, representing a hallmark feature of the disease. So far, most of the data on enthesitis in PsA are based on clinical assessment as well as MRI or ultrasound examinations, due to the challenge in retrieving good quality entheseal tissue for molecular analysis. However, pre-clinical studies as well as surgical invasive approaches on spondylarthritis patients showed that interleukin-17 (IL-17) is a key molecule in enthesitis pathogenesis, as also confirmed by the high clinical efficacy of IL-17 blockers in patients with this manifestation. Up to date, there are no molecular data assessing how IL-17 blockade ameliorates enthesitis in human. Therefore, deep omics analysis of these ultra-specialized tissues might expand our insights on PsA pathogenesis as well as provide information on treatment response. The aim of the study is to investigate the immune and the non-immune compartment in active enthesitis of PsA patients and their changes upon IL-17A neutralization.

Methods: Methods:

Minimal-invasive ultrasound-guided biopsy of the lateral epicondyle was performed, as previously described1, on 10 PsA patients with active elbow enthesitis before and after at least 3 months of treatment with secukinumab. All patients were biologic naïve at time of inclusion. Only one patient did not receive the second biopsy. Once harvested, the samples were fixed and conventional histology staining as well as second harmonic generation (SHG) were performed for morphologic evaluation and entheseal region identification1. Further slides were used for protein expression, through Hyperion imaging mass cytometry (IMC) and for RNA expression, through GeoMx spatial transcriptomic technology.

Results: 40% of the patients included into the study were female with an average age of 54 (± 9 SD). At the time of screening DAPSA, LEI and SPARCC were (mean ± SD) 23 ± 12, 2 ± 1 and 4 ± 3, respectively and by the end of the study (week 28), 11 ± 10, 1 ± 1, 1 ± 1, respectively. Entheseal region within the biopsies was identified by SHG. Neutrophils, CD4+T cells and ILC significantly decreased upon IL-17 blockade (p=0.017, 0.017, 0.021, respectively), with particular respect to the IL-17A positive fractions of each cell type. Beside quantitative reduction of IL-17-producing immune cells, major effects have been observed on transcriptional states of resident entheseal cells.

Spatial deconvolution analysis revealed a significant increase in the abundance of pro-resolving CD200+ fibroblasts2 and ILC2 cells following treatment (p=0.055, 0.049, respectively). Moreover, these cells were found to spatially colocalize together and with MERTK+ macrophages (CD200+ R=0.57 p=0.0041, ILC2 R=0.39 p=0.063), while dissociating with CD4+ T cells (CD200+ R=-0.74 p=0.000, ILC2 R=-0.64 p=0.001).

Conclusion: Here we show for the first time a full picture of cellular changes in the microarchitecture of inflamed entheses upon anti-IL-17 treatment. Resolving entheseal inflammation was not only associated with a reduction of the cellular load of IL-17-producing cells but also with tissue intrinsic modulations towards a pro-resolving microenvironment upon treatment with IL-17 inhibitors.


Disclosures: M. Raimondo: None; H. Mohammadian: None; S. Alivernini: None; S. Rauber: None; F. Fagni: None; G. Corte: None; K. Tascilar: None; M. D´Agostino: None; G. Schett: Bristol-Myers Squibb(BMS), 6, Cabaletta, 6, Janssen, 6, Kyverna Therapeutics, 6, Novartis, 6; A. Kleyer: None; A. Ramming: None.

To cite this abstract in AMA style:

Raimondo M, Mohammadian H, Alivernini S, Rauber S, Fagni F, Corte G, Tascilar K, D´Agostino M, Schett G, Kleyer A, Ramming A. Results from the Biopsy Driven Ebio Study: Entheseal Tissue Signature in Response to IL-17 Blockade in Psoriatic Arthritis [abstract]. Arthritis Rheumatol. 2024; 76 (suppl 9). https://acrabstracts.org/abstract/results-from-the-biopsy-driven-ebio-study-entheseal-tissue-signature-in-response-to-il-17-blockade-in-psoriatic-arthritis/. Accessed .
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