ACR Meeting Abstracts

ACR Meeting Abstracts

Abstract Number: 827

Reliability of the Quantitative Assessment of Peripheral Blood Perfusion By Laser Speckle Contrast Analysis in a Systemic Sclerosis Cohort

Valérie Lambrecht1, Maurizio Cutolo2, Filip De Keyser3, Saskia Decuman4, Barbara Ruaro2, Alberto Sulli5, Ellen Deschepper6 and Vanessa Smith7, 1Department of Rheumatology, Ghent University Hospital, Ghent, Belgium, 2Research Laboratory and Academic Division of Clinical Rheumatology, Department of Internal Medicine, University of Genova, Genova, Italy, 3Rheumatology, Ghent University Hospital, Ghent, Belgium, 4Department of Rheumatology, UGent, Ghent, Belgium, 5Research Laboratory and Academic Division of Clinical Rheumatology, Department of Internal Medicine, Genova, Italy, 6Biostatistics Unit, Biostatistics Unit, Ghent University, Ghent, Belgium, 7Department of Rheumatology, Ghent University Hospital, Ghent University, Ghent, Belgium

Meeting: 2015 ACR/ARHP Annual Meeting

Date of first publication: September 29, 2015

Keywords:

Session Information

Date: Sunday, November 8, 2015

Title: Systemic Sclerosis, Fibrosing Syndromes and Raynaud's - Clinical Aspects and Therapeutics Poster I

Session Type: ACR Poster Session A

Session Time: 9:00AM-11:00AM

Background/Purpose:

Laser speckle contrast analysis (LASCA) is an innovative technique to quantify peripheral blood perfusion (PBP) over a given area. Quantification of blood flow might be of interest in diseases with affection of the microcirculation e.g. systemic sclerosis (SSc). In order to be used as an outcome measure, first reliability of LASCA needs to be attested. This study investigates the reliability of the quantitative assessment of PBP by LASCA between two raters in a SSc cohort.

Methods:

Two independent raters evaluated PBP in 40 consecutive patients with SSc (all fulfilling the 2013 ACR/EULAR classification criteria) using LASCA (Pericam PSI, Perimed, Jarfalla). PBP was measured at the level of the fingertips, dorsal and volar bilaterally, for 30 sec each. Measurements were performed under standardized conditions (acclimatization in a temperature controlled darkened room at 25°C for 20 min; seated position; hand at heart level on flat underground; working distance 14.3-14.8 cm; no movement or speaking during measurement; time interval between two raters maximal 30 min). PBP values were reported as perfusion units (PU) by creating regions of interest with fixed 1 cm diameter at central area of 2nd, 3rd, 4th and 5th fingertips. Mean PU per finger, left and right, dorsal and volar, per rater was calculated. Inter-rater agreement was assessed by calculation of intraclass correlation coefficients (ICC). Patients with flexion contractures (n=6) were excluded because they could not meet the standardization requirements.

Results:

The mean PBP values (in PU) are described in Table 1. ICC (95% confidence interval) for PBP values measured by the two raters varied from 0.82 (0.67 to 0.91) to 0.91 (0.83 to 0.96) for the dorsal fingertips, and from 0.74 (0.55 to 0.86) to 0.86  (0.74 to 0.93) for the volar fingertips (Table 2).

Conclusion:

This is the first study to demonstrate reliability of the quantitative assessment of peripheral blood flow by LASCA in a SSc cohort between two raters, performed under standardized patient, instrumental and environmental conditions. Reliability of LASCA is essential for further use of this tool in SSc trials.

 Tabel 1 Mean (SD) PBP values (in PU) measured at level of fingertips in a SSc cohort

 

 

Rater 1

Rater 2

Dorsal

 

 

Left

 

 

F2

86.98 (49.57)

89.05 (47.44)

F3

80.89 (48.71)

85.68 (46.90)

F4

86.80 (54.94)

92.26 (56.81)

F5

92.36 (56.39)

96.26 (56.43)

Right

 

 

F2

109.25 (48.49)

109.74 (48.90)

F3

93.52 (50.87)

96.30 (50.27)

F4

98.33 (57.63)

104.16 (57.54)

F5

102.83 (53.61)

103.94 (58.73)

Volar

 

 

Left

 

 

F2

127.47 (73.57)

129.66 (76.51)

F3

115.35 (67.89)

117.61 (70.52)

F4

119.13 (62.82)

124.70 (71.90)

F5

116.15 (64.79)

119.01 (65.05)

Right

 

 

F2

149.91 (84.07)

153.95 (76.38)

F3

130.96 (71.12)

137.88 (71.81)

F4

142.42 (81.25)

150.51 (79.41)

F5

142.85 (76.89)

154.97 (79.48)

PBP, peripheral blood perfusion; PU, perfusion units; SSc, systemic sclerosis; F, fingertip.

Tabel 2 ICC (95% CI) for PBP measured by LASCA by two independent raters

 

 

ICC

95% CI

Dorsal

 

 

Left

 

 

F2

0.85

0.71;0.92

F3

0.89

0.79;0.94

F4

0.89

0.80;0.95

F5

0.83

0.68;0.91

Right

 

 

F2

0.91

0.83;0.96

F3

0.90

0.81;0.95

F4

0.88

0.77;0.94

F5

0.82

0.67;0.91

Volar

 

 

Left

 

 

F2

0.86

0.74;0.93

F3

0.85

0.72;0.92

F4

0.84

0.70;0.92

F5

0.80

0.63;0.89

Right

 

 

F2

0.84

0.70;0.91

F3

0.76

0.58;0.87

F4

0.74

0.55;0.86

F5

0.81

0.66;0.90

ICC, intraclass correlation coefficient; CI, confidence interval; PBP, peripheral blood perfusion; LASCA, laser speckle contrast analysis; F, fingertip.

Disclosure: V. Lambrecht, None; M. Cutolo, None; F. De Keyser, None; S. Decuman, None; B. Ruaro, None; A. Sulli, None; E. Deschepper, None; V. Smith, None.

To cite this abstract in AMA style:

Lambrecht V, Cutolo M, De Keyser F, Decuman S, Ruaro B, Sulli A, Deschepper E, Smith V. Reliability of the Quantitative Assessment of Peripheral Blood Perfusion By Laser Speckle Contrast Analysis in a Systemic Sclerosis Cohort [abstract]. Arthritis Rheumatol. 2015; 67 (suppl 10). https://acrabstracts.org/abstract/reliability-of-the-quantitative-assessment-of-peripheral-blood-perfusion-by-laser-speckle-contrast-analysis-in-a-systemic-sclerosis-cohort/. Accessed .

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