Background/Purpose:  To study the specific biomarkers by surface-enhanced laser desorption ionization/time of flight mass spectrometry (SELDI-TOF-MS) and protein chip in serum of patients with ankylosing spondylitis (AS). 

Methods:  The serum samples of 69 AS patients and 12 healthy individuals were detected by SELDI-TOF-MS and weak cation exchange (WCX-2) chip. To find the specific proteins and to set up the diagnostic models by using Biomarker Wizard and Biomarker Pattern software. Then 69 AS patients were divided into several types such as the condition of illness was active or not, HLA-B27 was positive or negative, with or without hip involvement. To study the possible roles of differentially expressed proteins in the pathogenesis of AS. 

Results:  The first diagnostic model (8085, 2640 and 2932)could be used to diagnose AS in early stage. The sensitivity and specificity were 94.23% and 100% respectively. The second diagnostic model (3677, 3880, 2539, 3159 and 3242) could be used to determine the disease activity of AS. The sensitivity and specificity were 98.11% and 100% respectively. The third diagnostic model (4700, 8687 and 18538) could be used to predict AS whether involve peripheral arthritis or not. The sensitivity and specificity were 80.00% and 82.35% respectively. The serum protein expression were not statistically difference between in the HLA-B27 positive group and HLA-B27 negative group.

Conclusion: The serum protein fingerprinting by SELDI-TOF-MS could identify new biomarkers in AS. The biomarkers may play an important role in pathogenesis of AS. We could diagnose AS in early stage, determine disease activity and predict disease progression by these biomarkers.

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ACR Meeting Abstracts - https://acrabstracts.org/abstract/protein-fingerprinting-screening-specific-proteins-in-serum-of-patients-with-ankylosing-spondylitis/