Session Type: ACR Poster Session B
Session Time: 9:00AM-11:00AM
The antiphospholipid syndrome (APS) is an autoimmune condition characterized by thrombosis, pregnancy complications and the presence of antiphospholipid antibodies. Basophils, which have long been associated with allergy and parasitic infections, are known to be activated and able to enhance the production of autoantibodies in autoimmune diseases, particularly in systemic lupus erythematosus (SLE). Herein we investigated the role of basophils in APS.
Peripheral basophils of patients with primary APS, SLE-associated APS (SLE-APS), and healthy controls (HC) were analyzed using flow cytometry.
Results: Forty-three consecutive patients with APS, including 10 patients with SLE-APS, and 20 HC were recruited. None of the patients was treated with corticosteroids or immunosuppressants. Thirty-five (81.4%) patients had lupus anticoagulant, 33 (76.7%) had anti-cardiolipin antibodies, and 24 (55.8%) had anti-ß2 glycoprotein I. Twenty (46.5%) patients had obstetric APS, 20 (46.5%) patients experienced arterial thrombosis and 19 (44.2%) patients experienced venous thrombosis. Six patients (14%) had a catastrophic APS (CAPS). Basophil activation markers revealed a decreased proportion of CD193+ basophils in patients with APS vs HC (63.0% [27.3 – 85.0] vs 90.4% [74.1 – 97.1]; p < 0.05) and an increased mean fluorescence intensity (MFI) of CD69 within basophils in patients with APS vs HC (839 [436 – 1434] vs 318 [103 – 755]; p < 0.05). Basophils functional markers revealed a decreased proportion of CD62L+ basophils in APS vs HC (99.0% [95.6 – 100] vs 100% [99.6 – 100]; p < 0.05), and an increased proportion of HLA-DR+ basophils in APS vs HC (32.3% [16.8 – 77.2] vs 10.0% [3.4 – 21.7]; p < 0.05). There was no difference between patients with APS and patients with SLE-APS, or patients with CAPS. When compared to patients without obstetric APS, patients with obstetric APS (46.5%) had a decreased proportion of CD196+ basophils (25.5% [11.3 – 32.5] vs 31.9% [25.8 – 66.3]; p < 0.05), an increased proportion of CD63+basophils (34.8% [0.25 – 53.3] vs 7.4% [0 – 37.9]; p < 0.05), and an increased proportion of HLA-DR+ basophils (49.0% [23.4 – 79.6] vs 20.8% [8.7 – 70.8]; p < 0.05). Lastly, the proportion of CD154+ basophils was higher in patients who experienced miscarriages than those who did not (47.6% [38.2 – 56.1] vs 19.7%[5.1 – 35.8]; p < 0.01) and MFI of basophil CD62L was higher in patients who experienced fetal death in utero than those who did not (11823 [9862 – 15546] vs 7978 [4628 – 10160]; p < 0.05).
Main phenotypic characteristics of basophils in APS are an increased expression of CD69 marker, a decreased expression of CD193 marker, and an increased expression of HLA-DR marker suggesting a role for basophils. Patients with obstetric APS differed from other APS patients by a skewed distribution of CD196+, CD63+ and HLA-DR+ basophils.
To cite this abstract in AMA style:Chaigne B, Le Guern V, Szwebel TA, Paule R, Le Jeunne C, Costedoat-Chalumeau N, Mouthon L. Phenotypic Characterization of Peripheral Basophil Perturbations in the Antiphospholipid Syndrome [abstract]. Arthritis Rheumatol. 2017; 69 (suppl 10). https://acrabstracts.org/abstract/phenotypic-characterization-of-peripheral-basophil-perturbations-in-the-antiphospholipid-syndrome/. Accessed June 5, 2020.
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ACR Meeting Abstracts - https://acrabstracts.org/abstract/phenotypic-characterization-of-peripheral-basophil-perturbations-in-the-antiphospholipid-syndrome/